Respirometry is the measurement of the biological oxygen consumption rate under well-defined conditions. This technique is generally employed to determine the kinetic and stoechiometric parameters of biological processes, such as: the oxygen transfer coefficient K L a, the endogenous oxygen uptake rate OUR end , exogenous oxygen uptake rate OUR exo , the total oxygen uptake rate OUR t ; the substrate affinity constant (K S ), the maximum growth rate (μ max ) and the yield (Y X/S ). According to the literature, there is a large variability in the estimation of these kinetic and stoechiometric parameters, attributed to three key factors: the culture history, the parameter identifiability and the initial substrate to biomass ratio (S 0 /X 0 ). K L a, depends on endogenous oxygen uptake rate OUR end , is a key constant in evaluating respirogramme-specific parameters. Generally, OUR end is assumed constant during dissolved oxygen variation. The effect of S 0 /X 0 ratio on kinetic and stoechiometric parameters has only been partially studied. The goal of this study was to assess respirometrically the effect of various S 0 /X 0 ratios (in the range of 0.01 to 0.23) on the kinetic and stoechiometric parameters of a heterotrophic activated sludge biomass (presence of ATU a nitrification inhibitor) and for both heterotrophicautotrophic activated sludge biomass.Key-words-Respirometry; S 0 /X 0 ratios; stoechiometric and kinetic parametres.
I.
A denitrification mathematical model was used to describe the nitrates and organic carbons use by the denitrify biomass in the Moving Bed Biofilm Reactor (MBBR). The model integrates the diffusive mass transfer mechanism as well as double substrates Monod kinetics. Preliminary experiments were realized in order to assess the operating conditions for growth of attached biomass, the determination of the optimal (COD/NO3-N) ratio, moreover the results of the previous study of the residence time distribution in this MBBR established the optimal hydrodynamic operating conditions with a dead volume of 22%. In this reactor, seeded with a mixed liquor from a purification station, kaldnesk1 were used as carriers. A total of 6 kinetic and stoichiometric constants under anoxic conditions were determined by batch-test pulsed respirometry; some parametric have been determined experimentally, such as YHD, YNO,
μ
ˆ
HD
${\widehat{\mu }}_{\text{HD}}$
and KS, and with their values 0.4 mgCOD (mgCOD)−1, 0.6 mg COD (mgCOD)−1,0.864 d−1 and 12.48 mg COD L −1, respectively. The other constants were determined using the model fitting (using MATLAB), such as KNO3 and bHD with its values, 0.25 mg NO3-N. L−1 and 0.061 d−1, respectively. The model was used to simulated different operating condition and the results included the concentration profiles of NO3-N, COD and XBH, which showed good agreement with the experimental ones, mainly by using the effective volume determined experimentally in the hydrodynamic study (RTD test) and which can reach 62% of the total volume under some operating. Additionally, these findings demonstrate that moving bed reactor characterization may be accomplished using in situ pulsed respirometry (MBBR).
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