A microplate fluorimetric assay for the study of enzyme diversity in soils

Marx, M.; Wood, M.; Jarvis, S. C.

doi:10.1016/s0038-0717(01)00079-7

Cited by 733 publications

(353 citation statements)

References 20 publications

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“…Reactions (50 ll) were prepared in 96-well microplates with 1-2 mg sample per well. A final substrate concentration of 0.1 mM was used in this study (Marx et al 2001). Reactions were incubated in an optical thermocycler (iQ iCycler, Bio-Rad, Hercules, California, USA) and final activity was expressed as the slope of the linear increase of fluorescence produced during the reaction time per dry weight at each temperature.…”

Section: Enzymatic Assaysmentioning

confidence: 99%

Latitude-dependent underestimation of microbial extracellular enzyme activity in soils

Gonzalez

Guisado

Piñeiro-Vidal

2014

Int. J. Environ. Sci. Technol.

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Decomposition of soil organic matter by microorganisms is a major process governing the carbon balance between soil and atmosphere which needs to be fully understood. Extracellular enzyme activity is often the limiting factor for microbial utilization of soil organic matter. Contrary to expectations, we observed that enzymatic activity rises at increasing temperatures in soils and sediments. Current climatic change will induce the increase of global mean temperatures, frequency of extreme heat events and soil temperatures during the next decades. The relevance of the increase in activity at high temperature is dependent on latitude. At latitudes around and below 40°a significant number of days per year present high temperatures. Results suggest that the hydrolytic activity of microbial extracellular enzymes is currently underestimated mainly at medium and low latitudes where soil temperatures frequently reach high values (often above 40°C). This report contributes to understand (1) the hydrolysis of soil organic matter within a latitude-dependent scenario of global warming and (2) the role of microorganisms in processing soil organic matter and their influence in carbon cycling.

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Section: Enzymatic Assaysmentioning

confidence: 99%

Latitude-dependent underestimation of microbial extracellular enzyme activity in soils

Gonzalez

Guisado

Piñeiro-Vidal

2014

Int. J. Environ. Sci. Technol.

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“…Five enzymes involved in C and nutrient cycling were measured using microplate fluorimetric assays with 200 lM methylumbelliferone (MU)-linked substrates (Marx et al 2001): b-glucosidase (degradation of labile C), cellobiohydrolase (degradation of cellulose), N-acetyl b-glucosaminidase (degradation of N-glycosidic bonds), phosphomonoesterase (degradation of monoester-linked simple organic phosphates) and xylanase (degradation of hemicellulose). Soil samples were collected from soil containers to 2 cm depth, stored at 3°C and assayed within 3 days, which does not appear to greatly alter observed activities in tropical forest soils (Turner and Romero 2010).…”

Section: Soil Nutrients Enzymes and Microbial Biomassmentioning

confidence: 99%

Priming and microbial nutrient limitation in lowland tropical forest soils of contrasting fertility

et al. 2011

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“…Assays were conducted using methylumbelliferone-linked fluorogenic substrates (Marx et al 2001). Enzymes (and substrates) were:…”

Section: Hydrolytic Enzyme Assaysmentioning

confidence: 99%

Biogeochemical processes along a nutrient gradient in a tropical ombrotrophic peatland

et al. 2010

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Biogeochemical properties, including nutrient concentrations, carbon gas fluxes, microbial biomass, and hydrolytic enzyme activities, were determined along a strong nutrient gradient in an ombrotrophic peatland in the Republic of Panama. Total phosphorus in surface peat decreased markedly along a 2.7 km transect from the marginal Raphia taedigera swamp to the interior sawgrass swamp, with similar trends in total nitrogen and potassium. There were parallel changes in the forest structure: basal area decreased dramatically from the margins to the interior, while tree diversity was greatest at sites with extremely low concentrations of readily-exchangeable phosphate. Soil microbial biomass concentrations declined in parallel with nutrient concentrations, although microbes consistently contained a large proportion (up to 47%) of the total soil phosphorus. Microbial C:P and N:P ratios and hydrolytic enzyme activities, including those involved in the cycles of carbon, nitrogen, and phosphorus, increased towards the nutrient-poor wetland interior, indicating strong belowground nutrient limitation. Soil CO 2 fluxes and CH 4 fluxes did not vary systematically along the nutrient gradient, although potential soil respiration determined on drained soils was lower from nutrient-poor sites. Soil respiration responded strongly to drainage and increased temperature. Taken together, our results demonstrate that nutrient status exerts a strong control on above and below-ground processes in tropical peatlands with implications for carbon dynamics and hence long term development of such ecosystems.

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A microplate fluorimetric assay for the study of enzyme diversity in soils

Cited by 733 publications

References 20 publications

Latitude-dependent underestimation of microbial extracellular enzyme activity in soils

Latitude-dependent underestimation of microbial extracellular enzyme activity in soils

Priming and microbial nutrient limitation in lowland tropical forest soils of contrasting fertility

Biogeochemical processes along a nutrient gradient in a tropical ombrotrophic peatland

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