A microfluidic microwell device operated by the automated microfluidic control system for surface-enhanced Raman scattering-based antimicrobial susceptibility testing

An antibiotic concentration gradient microfluidic device integrating surface-enhanced Raman spectroscopy for multiplex antimicrobial susceptibility testing

“…Based on the microscopy images, we found that the equivalent bacteria concentration is around 2.5–5 × 10 7 CFU mL −1 , which is similar to the bacteria concentration we used in our previous SERS-AST experiments. 13,34…”

“…36 A similar response was also observed in our previous study. 34 However, by using the ACGM device, we can better quantify this hormesis effect based on a continuous antibiotic concentration gradient.…”

Microfluidic nanodevices for drug sensing and screening applications

“…(c) Illustration of the hydrogel-based helical hollow microfibers to mimic the structural characteristics and swirl blood flow in the blood vessels, reproduced with permission from ( Li et al, 2019 , Li et al, 2019 ), copyright 2019 Wiley-VCH. (d) An olive-shaped microfluidic microwell device to encounter the bobble generation in SERS-AST protocol, reproduced with permission from ( Liao et al, 2021 ), copyright 2021 Elsevier. (e) A 3D advection-diffusion model of the microfluidic cavity using computational fluid dynamics (CFD), reproduced with permission from ( Kohl et al, 2021 ), copyright 2021 Wiley-VCH.…”

“…In the SERS-AST protocol, bubble generation during air isolation is a critical problem that can hamper the detection process. Herein, the olive-like geometry plays the most critical role in eliminating bobble generation ( Liao et al, 2021 ). Hence, directly or indirectly, the geometry of the microfluidic system is one of the most critical factors in drug screening applications.…”

Recent advances in antibiotic resistance diagnosis using SERS: focus on the “Big 5” challenges

“…6). 90 This in situ SERS-AST was performed by loading isolates of susceptible and resistant E. coli with ampicillin for 3.5 hours. A clear discrimination between the two strains under antibiotic treatment was obtained.…”