An antibiotic concentration gradient microfluidic device integrating surface-enhanced Raman spectroscopy for multiplex antimicrobial susceptibility testing

Lin, Shang-Jyun; Chao, Po-Hsuan; Cheng, Ho-Wen; Wang, Juen-Kai; Wang, Yuh‐Lin; Han, Yin-Yi; Huang, Nien-Tsu

doi:10.1039/d2lc00012a

Cited by 23 publications

(19 citation statements)

References 35 publications

(34 reference statements)

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“…Lin et al developed microfluidic devices integrating SERS for antimicrobial susceptibility testing . The more recent version of the devices was designed to load and confine bacteria into an array of microwells and generate a concentration gradient of the antibiotics within the wells for multiparallel testing of the minimum inhibitory concentration . After bacteria incubation in varying concentrations of the antibiotics, the top layer of the device was replaced with a SERS substrate to measure SERS signals of bacterial metabolites.…”

Section: Sensor and Detection Technologymentioning

confidence: 99%

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Aubry

Lee

2023

Anal. Chem.

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Section: Sensor and Detection Technologymentioning

confidence: 99%

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Aubry

Lee

2023

Anal. Chem.

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“…SERS-based AST can eliminate the need for overnight culture and reduce the testing time, as it can rapidly assess the response and the susceptibility of bacteria to the antibiotic treatments. 83–85 In turn, this will be helpful in understanding the mechanism of action and effectiveness of antibiotics in a short time and allow a rapid decision to be taken by the physicians. 86…”

Section: Sers For Antibiotic Resistance Diagnosismentioning

confidence: 99%

Recent advances in antibiotic resistance diagnosis using SERS: focus on the “Big 5” challenges

Hassanain

Johnson

Faulds

et al. 2022

Analyst

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“…To stabilize subjects, for example, cells in the middle observation chamber, the low-height channel [ 17 , 18 ], the membrane [ 19 ] and gels [ 20 , 21 ] were employed to form the obstacles. The unique advantage of high integration makes it possible for microfluidics to contain an array of chambers for high-through gradient analysis using limited samples [ 22 , 23 , 24 , 25 ]. However, the self-diffusion-based approach can only maintain a simple gradually decreasing concentration between a chemical source and a sink channel.…”

Section: Introductionmentioning

confidence: 99%

Generation of Dynamic Concentration Profile Using A Microfluidic Device Integrating Pneumatic Microvalves

Chen

et al. 2022

Biosensors

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Generating and maintaining the concentration dilutions of diffusible molecules in microchannels is critical for high-throughput chemical and biological analysis. Conventional serial network microfluidic technologies can generate high orders of arbitrary concentrations by a predefined microchannel network. However, a previous design requires a large occupancy area and is unable to dynamically generate different profiles in the same chip, limiting its applications. This study developed a microfluidic device enabling dynamic variations of both the concentration in the same channel and the concentration distribution in multiple channels by adjusting the flow resistance using programmable pneumatic microvalves. The key component (the pneumatic microvalve) allowed dynamic adjustment of the concentration profile but occupied a tiny space. Additionally, a Matlab program was developed to calculate the flow rates and flow resistance of various sections of the device, which provided theoretical guidance for dimension design. In silico investigations were conducted to evaluate the microvalve deformation with widths from 100 to 300 µm and membrane thicknesses of 20 and 30 µm under the activation pressures between 0 and 2000 mbar. The flow resistance of the deformed valve was studied both numerically and experimentally and an empirical model for valve flow resistance with the form of Rh=aebP was proposed. Afterward, the fluid flow in the valve region was characterized using Micro PIV to further demonstrate the adjustment mechanism of the flow resistance. Then, the herringbone structures were employed for fast mixing to allow both quick variation of concentration and minor space usage of the channel network. Finally, an empirical formula-supported computational program was developed to provide the activation pressures required for the specific concentration profile. Both linear (Ck = −0.2k + 1) and nonlinear (Ck = (110)k) concentration distribution in four channels were varied using the same device by adjusting microvalves. The device demonstrated the capability to control the concentration profile dynamically in a small space, offering superior application potentials in analytical chemistry, drug screening, and cell biology research.

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An antibiotic concentration gradient microfluidic device integrating surface-enhanced Raman spectroscopy for multiplex antimicrobial susceptibility testing

Abstract: Antimicrobial susceptibility testing (AST) are a key measure in clinical microbiology laboratories to enable appropriate antimicrobial administration. During an AST, the determination of the minimum inhibitory concentration (MIC) is an...

Cited by 23 publications

References 35 publications

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Recent advances in antibiotic resistance diagnosis using SERS: focus on the “Big 5” challenges

Generation of Dynamic Concentration Profile Using A Microfluidic Device Integrating Pneumatic Microvalves

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