A microfluidic chip for ammonium sensing incorporating ion-selective membranes formed by surface tension forces

Chiang, Ting-Yi; Lin, Che-Hsin

doi:10.1039/c3ra45809a

Cited by 13 publications

(10 citation statements)

References 28 publications

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“…For this challenge, electrical transduction methods are more suitable for the detection of ionic species (oxidable or reducable) because of their high sensitivity and no need labelling step is required [4][5][6]. The relevance of electrochemical detection on microchips with improvement of micro nanoelectrode fabrication [7][8][9][10][11][12] has been widely proven, including the application of amperometric detection [13][14][15][16], and potentiometric [17][18][19]. However, contactless to the microelectrodes as electrical detection scheme with the electrolyte is the most promising [20][21][22][23].…”

Section: Introductionmentioning

confidence: 98%

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Kechadi

Sotta

Gamby

2015

Talanta

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This paper presents the use of polymer coated microelectrodes for the realtime conductivity monitoring in a microchannel photoablated through the polymer without contact. Based on this strategy, a small conductometry sensor has been developed to record in time conductivity variation when an enzymatic reaction occurs through the channel. The rate constant determination, k2, for the dephosphorylation of organic phosphate-alkaline phosphatase-superparamagnetic beads complex using chemically different substrates such as adenosine monoesterphosphate, adenosine diphosphate and adenosine triphosphate was taken as an example to demonstrate selectivity and sensivity of the detection scheme. The k2 value measured for each adenosine phosphate decreases from 39 to 30 s(-1) in proportion with the number (3, 2 and 1) of attached phosphate moiety, thus emphasizing the steric hindrance effect on kinetics.

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Section: Introductionmentioning

confidence: 98%

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Kechadi

Sotta

Gamby

2015

Talanta

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“…Micro-scale technologies have been previously used for detection of non-biological contaminants such as pesticides [266,267], phosphate [268], Hg in water [269], ammonium ion [270] and As ions [271]. In addition, this technology has shown tremendous LOD improvements of biological contaminants.…”

Section: Microfluidic Sensorsmentioning

confidence: 99%

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Zulkifli

Rahim

Lau

2018

Sensors and Actuators B: Chemical

217

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a b s t r a c tWater monitoring technologies are widely used for contaminants detection in wide variety of water ecology applications such as water treatment plant and water distribution system. A tremendous amount of research has been conducted over the past decades to develop robust and efficient techniques of contaminants detection with minimum operating cost and energy. Recent developments in spectroscopic techniques and biosensor approach have improved the detection sensitivities, quantitatively and qualitatively. The availability of in-situ measurements and multiple detection analyses has expanded the water monitoring applications in various advanced techniques including successful establishment in hand-held sensing devices which improves portability in real-time basis for the detection of contaminant, such as microorganisms, pesticides, heavy metal ions, inorganic and organic components. This paper intends to review the developments in water quality monitoring technologies for the detection of biological and chemical contaminants in accordance with instrumental limitations. Particularly, this review focuses on the most recently developed techniques for water contaminant detection applications. Several recommendations and prospective views on the developments in water quality assessments will also be included.

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“…9 Microfluidic devices integrated with the ion-selective membrane or modified nano-electrodes for high-performance analyzing the concentration of urea and glucose (GLU) in human serum were also reported. 9,10 However, the fabrication for the sensing electrodes was comparatively delicate. Moreover, most biochip devices aimed to perform one biocatalytic reaction and detection in one test.…”

Section: Introductionmentioning

confidence: 99%

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood

Yang

Lin

2015

Biomicrofluidics

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This research presents a multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen (BUN) and glucose detection in human whole blood. A novel enzyme-doped thread coated with a thin polyvinylchloride (PVC) membrane is produced for on-site electrochemical detection of urea and glucose in whole blood. Multiple enzymes can be directly applied to the thread without delicate pretreatment or a surface modification process prior to sealing the thread with PVC membrane. Results indicate that the developed device exhibits a good linear dynamic range for detecting urea and glucose in concentrations from 0.1 mM-10.0 mM (R(2 )= 0.9850) and 0.1 mM-13.0 mM (R(2 )= 0.9668), which is suitable for adoption in detecting the concentrations of blood urea nitrogen (BUN, 1.78-7.12 mM) and glucose (3.89-6.11 mM) in serum. The detection result also shows that the developed thread-based microfluidic system can successfully separate and detect the ions, BUN, and glucose in blood. The calculated concentrations of BUN and glucose ante cibum (glucose before meal) in the whole blood sample are 3.98 mM and 4.94 mM, respectively. The developed thread-based microfluidic system provides a simple yet high performance for clinical diagnostics.

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A microfluidic chip for ammonium sensing incorporating ion-selective membranes formed by surface tension forces

Cited by 13 publications

References 28 publications

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood

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