A method to measure dual NK1/NK2-antagonist activity in dogs

Intestinal motor stimulation by the 5‐HT4 receptor agonist ML10302: differential involvement of tachykininergic pathways in the canine small bowel and colon

“…Our study was designed to assess whether or not tachykinins are involved in mediating the response to ML10302. To this end, the antagonists must ensure full blockade of NK receptors throughout the experiment, as documented in the literature for the dose of 1 mg kg –1 26–28 , 30–32 . The fact that none of the antagonists, at this dose, affected the response to ML10302 in the upper gut (duodenum or jejunum) in our sample of 10 dogs strongly indicates that tachykinins are not involved in mediating the response at this level.…”

“…injection of each tachykinin receptor antagonist (SR140333, SR48968 or SR142801) per se at the dose of 1 mg kg –1 was also tested. For each tachykinin receptor antagonist, the selected dose provides full blockade of the relevant receptor 26–28 , 30–32 . The doses of atropine were chosen on the basis of a previous dose–response study, showing that they provided full and partial blockade of muscarinic transmission, respectively 29…”

Bronchoconstrictor Reactivity to NKA in Allergic Dogs: a Comparison to Histamine and Methacholine

“…6 Inhaled NKA produces bronchoconstriction in human asthmatics, but has little or no effect in normal subjects. [7][8][9][10][11] In dogs, inhaled NKA produces METHODS bronchoconstriction in a sub-population of beagle dogs with normal, healthy lungs, [12][13][14] but bronchoAnimal sources constrictor reactivity to NKA has not been assessed in canine airways in the presence of atopy or pulmonary Studies were performed in two groups of beagle dogs disease.…”

“…At the end of the used for studies involving bronchoprovocation with aerosol challenge, the endotracheal tube was reaerosolized NKA. [12][13][14] The dogs in this population connected to the pneumotachograph and pulmonary were all uncastrated males. functions were measured during spontaneous respiration for 3 min after each aerosol challenge.…”

“…In this regard, studied, although doses of NKA higher than those Antoinssen et al 18 demonstrated that isolated airway used in the present studies increase ventilation in preparations obtained from allergen-sensitized dogs dogs. [12][13][14] Interestingly, the tachypnea response to hispossessed bronchial hyperresponsiveness compared to tamine challenge was heightened in the ragweed-sensnon-sensitized controls. Third, it is possible that airitized dogs 24 h after antigen challenge.…”