A Method of Isolating the Four Spores from a Single Basidium in Schizophyllum Commune

Papazian, Haig P.

doi:10.1086/335638

Cited by 13 publications

(9 citation statements)

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“…After about 24 hours, well-separated germlings were selected and transferred to agar slants. In some instances products of one basidium were isolated with a micromanipulator (Fonbrune), using the method of PAPAZIAN (1950).…”

Section: Methodsmentioning

confidence: 99%

“…Schizophyllum shares this pattern of sexuality, commonly called tetrapolar multiple allelomorph heterothallism, with 55 Ufo of 230 species of Hymenomycetes and Gasteromycetes investigated (WHITEHOUSE, 1949). In matings between mycelia bearing common A or B factors, special interactions occur which show that the two mating-type loci control different parts of the process of dikaryon formation (PAPAZIAN, 1950;PARAG, 1962). If the mycelia have common A factors and different B factors, a so-called "flat" heterokaryon is set up.…”

Section: Introductionmentioning

confidence: 99%

“…Another argument stems from the fact that more than two allelomorphs of the mating-type loci have never been recorded for the spores of a single fruit body of Schizophyllum or other Hymenomycetes, except for instances in which some of the spores show departure from this rule. This inconsistency was originally attributed to mutation at the mating-type loci, (KNIEP, 1923), later to inter-subunit crossing-over (PAPAZIAN, 1951;RAPER et al, 1960). Thus, it is evident that the progeny arise from basidia, all of which have the same genetic constitution.…”

Section: Introductionmentioning

confidence: 99%

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Morphogenesis and Biochemical Processes in Schizophyllum Commune Fr.

Wessels

1965

Wentia

142

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The investigation concerns a first attempt to unravel biochemical patterns of significance for morphogenesis in S. commune. The results derive from two different stocks: one with wild morphology, the other exhibiting an impairment of pileus formation. Some morphological and genetical features of the stocks are discussed. A special culture technique allowed fair synchronization and control of development via the medium. This technique is based on the fact that differentiation can be prevented by cultivation in a thiamine‐free medium. Measured changes in protein, RNA, DNA, and soluble cell constituents during thiamine‐induced primordia formation yielded some information concerning the first steps of differentiation. Primordial growth was found to depend upon transport of nitrogenous compounds from the mycelium and the presence of a carbon source in the medium. Most of the assimilated glucose is deposited as polysaccharides in the cell walls of the primordia. Chemical analysis of the cell walls demonstrated that they are mainly composed of two different glucans, called here S‐ and R‐glucan, some properties of which were investigated. Pileus formation was shown to occur without the uptake of nutrients from the medium and to depend upon the withdrawal of substances from stunted fructifications and stroma in particular. Comparison of the two investigated stocks provided strong support for a causal relationship between degradation of the cell‐wall glucans and pileus formation. An analysis was made of respiration and fermentation during development; some findings probably bearing upon the problem of morphogenesis are discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphogenesis and Biochemical Processes in Schizophyllum Commune Fr.

Wessels

1965

Wentia

142

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“…Raper 1963, Seaby 1977, Messner 1980. Methods have also been described for the isolation of basidiospore tetrads, with a micromanipulator (Papazian 1950, Moore 1966 or by hand (Epp 1977) .…”

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confidence: 99%

Simple isolation and inoculation methods for fungal cultures

Korhonen¹,

Hintikka²

1980

Karstenia

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“…The postulated genotypes of these strains are represented by the allelomorph symbols s, d", d 5 , d 6 and combinations thereof. A basic medium which contained glu--cose as an energy source, 0.2 per cent peptone and inorganic salts (Papazian, 1950a) was used throughout. The methods used for the production of fruit-1 Received for publication October 8, 1954.…”

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confidence: 99%