Simple isolation and inoculation methods for fungal cultures

Korhonen, Kari T.; Hintikka, Veikko

doi:10.29203/ka.1980.192

Cited by 41 publications

(18 citation statements)

References 10 publications

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“…Rhizomorphs were surface sterilized for 2–3 min in 20% ethanol followed by 1% NaOCl (diluted from a commercial 5% stock solution) for 5 min, before rinsing in three changes of sterile water and plated on PDA, MEA or CA (B lodget and W orrall 1992). Single‐basidiospore isolations were prepared on 1 and 2% MEA, using the method of K orhonen and H intikka (1980). All cultures were incubated in darkness at 24°C.…”

Section: Methodsmentioning

confidence: 99%

Distribution, ecology and host range of Armillaria species in Albania

et al. 2010

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Summary Species of Armillaria were identified from 645 isolates obtained in a nation‐wide survey in Albania. The material was collected from ca. 250 permanent plots, established for monitoring forest health, and from forests and orchards attacked by Armillaria. Armillaria mellea s.s. occurred on several coniferous and broadleaved trees in most areas examined, although it was absent above 1100–1200 m in northern Albania. This species damaged Abies and Quercus spp. and, to a lesser extent, other forest trees. Armillaria mellea was also commonly recorded causing damage in orchards and vineyards. Armillaria gallica was a common saprophyte or weak pathogen in coniferous and deciduous forests at altitudes from 600 to 1600 m, and less commonly on oaks at lower altitudes. Armillaria ostoyae was rare in central and southern Albania, but common in northern Albania, causing significant damage to pine and other conifers, mostly at altitudes from 600 to 1800 m. Armillaria cepistipes was recorded at altitudes from 800 to 1800 m as a saprophyte or weak pathogen on conifers and deciduous trees, mostly in beech and silver fir forests. Armillaria tabescens was found in oak forests at altitudes from sea level to 900 m. In orchards, A. tabescens occasionally attacked almond and pear trees. Armillaria borealis was found in a few locations in northern Albania, at altitudes from 800 to 1800 m.

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Section: Methodsmentioning

confidence: 99%

Distribution, ecology and host range of Armillaria species in Albania

et al. 2010

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“…Five European annulate Armillaria species were distinguished 30 years ago based on intersterility tests (Korhonen 1978;Korhonen and Hintikka 1980). Since then, thorough anatomical and morphological studies (including Antonín 1986Antonín , 1990Marxmüller 1992;Termorshuizen and Arnolds 1997;Volk and Burdsall 1995) have further differentiated the taxonomy of the group.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characterization of the Armillaria cepistipes – A. gallica complex in the Czech Republic and Slovakia

et al. 2009

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Armillaria cepistipes and A. gallica (Basidiomycota, Physalacriaceae) are morphologically similar species, and they are often nearly indistinguishable using DNAbased methods targeting the ITS region of ribosomal DNA. The aim of this study was to examine morphological and ecological features of A. cepistipes and A. gallica, and to test other DNA-based methods to distinguish the two species. Our results revealed discriminative macro-and micromorphological features between these two species, especially the presence of a distinct central pileus ocella, the shape of the annulus, the character of the velar stipe remnants and the length of the terminal cells of the pileus scales. Ecologically, A. gallica generally prefers warmer areas in lowlands (oak and alluvial forests), while A.cepistipes is more common in hilly and lower montane beech forests in Central Europe. Nevertheless, despite differences in ecological preferences, certain locations between 300 and 500 m a.s.l. are known to sympatrically support both species. The sequences of the translation elongation factor 1-alpha showed high interspecific variability, and this gene is a more appropriate candidate for distinguishing A. gallica from A. cepistipes.

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“…The penultimate cell of a clampless hyphal tip was cut with a tiny glass knife and the isolated tip cell was picked up with a modified Pasteur pipette (Korhonen and Hintikka, 1980). The uninucleate condition of each isolated cell was confirmed with phase contrast microscopy.…”

Section: Matings and Isolation Of Diploid Cellsmentioning

confidence: 99%