A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation

Guo, Guanhua; Jin, Linhe; Wu, Binggang; He, Haiyang; Yang, Fan; Xu, Liangpeng; Lei, Yang; Wang, Yunbing

doi:10.1016/j.actbio.2020.10.029

Cited by 28 publications

(30 citation statements)

References 69 publications

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“…The Mimicking of the behavior of HUVECs is essential to evaluate the cytocompatibility and toxicity of bioprosthetic heart valves. As our results showed, both cell adhesion and proliferation tests were better in the NPTD group than that in the GA group, suggesting that the hybrid cross-linking method has the characteristics of low toxicity, which provides an opportunity for endothelialization and regeneration of BHVs in vivo (Guo et al, 2021). Heart valves control unidirectional blood flow within the heart during the heart cycle which experience three distinct states of tissue stress: tension, bending, and shear stress.…”

Section: Figurementioning

confidence: 76%

“…It should also be noted that calcification could accelerate degeneration of tissue. In addition, the drawbacks of residual toxicity, inflammatory reaction are frequently met in Glut crosslinked valves (Liu et al, 2019;Guo et al, 2021).…”

Section: Figurementioning

confidence: 99%

“…Collagen treated with GA is a strong calcium nucleating agent. (Guo et al, 2018;Guo et al, 2021). Calcification of collagen can be blocked by changing the cross-linking agent.…”

Section: Figurementioning

confidence: 99%

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The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves

Liu

Wu²,

Chen³

et al. 2022

Front. Bioeng. Biotechnol.

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The bioprosthetic heart valves (BHVs) are the best option for the treatment of valvular heart disease. Glutaraldehyde (Glut) is commonly used as the golden standard reagent for the crosslinking of BHVs. However, the obvious defects of Glut, including residual aldehyde toxicity, degradation and calcification, increase the probability of valve failure in vivo and motivated the exploration of alternatives. Thus, the aim of this study is to develop a non-glutaraldehyde hybrid cross-linking method composed of Neomycin Trisulfate, Polyethylene glycol diglycidyl ether and Tannic acid as a substitute for Glut, which was proven to reduce calcification, degradation, inflammation of the biomaterial. Evaluations of the crosslinked bovine pericardial included histological and ultrastructural characterization, biomechanical performance, biocompatibility and structural stability test, and in vivo anti-inflammation and anti-calcification assay by subcutaneous implantation in juvenile Sprague Dawley rats. The results revealed that the hybrid crosslinked bovine pericardial were superior to Glut crosslinked biomaterial in terms of better hydrophilicity, thermodynamics stability, hemocompatibility and cytocompatibility, higher Young’s Modulus, better stability and resistance to enzymatic hydrolysis, and lower inflammation, degradation and calcification levels in subcutaneous implants. Considering all above performances, it indicates that the hybrid cross-linking method is appropriate to replace Glut as the method for BHV preparation, and particularly this hybrid crosslinked biomaterials may be a promising candidate for next-generation BHVs.

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Section: Figurementioning

confidence: 76%

Section: Figurementioning

confidence: 99%

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The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves

Liu

Wu²,

Chen³

et al. 2022

Front. Bioeng. Biotechnol.

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“…To verify the hemocompatibility of GA-HA, comprehensive evaluations were performed (Figures , , and ). FBG and BSA were two commonly used proteins to detect the protein adhesion property of materials. , FBG was involved in the coagulation cascade by specifically reacting with thrombin to cause coagulation, and serum albumin was the most abundant protein in the blood plasma. , The result of the protein adhesion test showed that GA-HA had better resistance for protein adhesion, especially for non-specific protein (BSA), which might be attributed to the HA-grafting. Previous studies have shown that the mechanisms for the improved antithrombogenicity by HA modification mainly include: grafted HA could (1) cover the irregular surface, which could reduce the sites for the formation of thrombus and (2) reduce the concentration of procoagulants and platelet activating factors under unidirectional laminar blood flow .…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid-Grafted Bioprosthetic Heart Valves Achieved by Copolymerization Exhibited Improved Anticalcification and Antithrombogenicity

Huang

Cheng

Ding

et al. 2022

ACS Biomater. Sci. Eng.

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Bioprosthetic heart valves (BHVs) are widely used in clinic, but they still have problems of calcification, thrombogenicity, and cytotoxicity. The reported techniques based on glutaraldehyde (Glut) crosslinking have difficulty in solving these problems simultaneously. In this study, we grafted Glut-crosslinked porcine pericardium (GA) with hyaluronic acid (HA) by radical copolymerization to improve its anticalcification and antithrombotic properties. Partially methacrylated poly-εlysine was used to introduce methacryl groups into GA. Then, HAgrafted porcine pericardium (GA-HA) was obtained by radical copolymerization. Rat's subcutaneous implantation results showed that the calcium content of GA-HA was significantly lower than that of GA (37 ± 29 μg/mg vs 188 ± 7 μg/mg), and the platelets adhering to the surface of GA-HA decreased by approximately 41% compared with GA. In conclusion, grafting porcine pericardium with HA by copolymerization might be feasible to improve the anticalcification and antithrombotic properties of BHVs.

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“…According to reports, glutaraldehyde can react with hydroxyl to form compounds under acidic conditions. [19][20][21] For example, Ariel W. Chan 22 used acid-catalyzed acetalization reaction to chemically modify alginate with glutaraldehyde, and they wanted to enable the control of properties generally not possible with the native polymer by chemically modify alginate. Therefore, under acidic conditions, glutaraldehyde can effectively react with the hydroxyl groups on heparin.…”

Section: Introductionmentioning

confidence: 99%

A heparin-functionalized covered stent prepared by plasma technology

et al. 2021

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In this study, the surface of the covered stent was treated by plasma technology to introduce amino functional groups, and glutaraldehyde and heparin were successfully grafted to prepare a heparin-functionalized covered stent (HPLCS). The preparation parameters such as plasma treatment power, plasma treatment time, concentration of glutaraldehyde and heparin, and pH of heparin solution were studied in detail. The functionalized heparin covered stent can make the titer of heparin reach 1.23 ± 0.03 IU/cm2. In animal experiments, after implantation in pigs for 6 months, the titer of heparin can still reach 0.93 ± 0.05 IU/cm2. This work provides a good method for preparing heparin covered stent.

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A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation

Cited by 28 publications

References 69 publications

The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves

The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves

Hyaluronic Acid-Grafted Bioprosthetic Heart Valves Achieved by Copolymerization Exhibited Improved Anticalcification and Antithrombogenicity

A heparin-functionalized covered stent prepared by plasma technology

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