2016
DOI: 10.3390/ijms17111880
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A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses

Abstract: Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute respiratory infection. They have a global distribution. Rapid and accurate diagnosis of HCoV infection is important for the management and treatment of hospitalized patients with HCoV infection. Here, we developed a melting curve-based multiplex RT-qPCR assay for simultaneous detection of the four HCoVs. In the assay, SYTO 9 was used to replace SYBR Green I as the fluorescent dye, and GC-modif… Show more

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Cited by 79 publications
(67 citation statements)
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“…Coronaviruses are enveloped, nonsegmented, positive, single-stranded RNA viruses associated with the structural N-phosphoprotein in a nucleocapsid with helical symmetry 112,113 . They are found in a wide variety of animals causing respiratory, enteric, hepatic, and neurological diseases of varying severity 114 . According to the International Committee on Taxonomy of Viruses, two subfamilies belong to the Coronaviridae family; Letovirinae, which has one subgenus, Milecovirus, found only in frogs and a sea hare thus far 115 , and Orthocoronavirinae, which is found in birds and mammals, and is divided into four genera due to the antigenic and genetic characteristics of the viruses 53,116 .…”
Section: Coronavirus (Cov)mentioning
confidence: 99%
See 1 more Smart Citation
“…Coronaviruses are enveloped, nonsegmented, positive, single-stranded RNA viruses associated with the structural N-phosphoprotein in a nucleocapsid with helical symmetry 112,113 . They are found in a wide variety of animals causing respiratory, enteric, hepatic, and neurological diseases of varying severity 114 . According to the International Committee on Taxonomy of Viruses, two subfamilies belong to the Coronaviridae family; Letovirinae, which has one subgenus, Milecovirus, found only in frogs and a sea hare thus far 115 , and Orthocoronavirinae, which is found in birds and mammals, and is divided into four genera due to the antigenic and genetic characteristics of the viruses 53,116 .…”
Section: Coronavirus (Cov)mentioning
confidence: 99%
“…Betacoronaviruses are found mainly in mammals, such as humans, dogs, cats, pigs, bats, mice, rats, horses, and cattle 114,[118][119][120][121][122][123] , while Gama and Deltacoronaviruses infect mainly birds, with exceptions such as the white whale Gamacoronavirus (Delphinapterus leucas) 124 and the porcine Deltacoronavirus 125 .…”
Section: Coronavirus (Cov)mentioning
confidence: 99%
“…Real-time reverse transcriptase-PCR (RT-PCR) detection is currently favored for the detection of coronavirus because of its advantages as a specific, and simple quantitative assay. Moreover, realtime RT-PCR is more sensitive than the conventional RT-PCR assay, which help much for the diagnosis in early infection [10,11]. Therefore, the real-time RT-PCR assay still is a predominant method to be applied for the detection of all kinds of coronaviruses [12,13], including SARS-CoV-2 [14].…”
Section: Pcr-based Methodsmentioning
confidence: 99%
“…Among nucleic acid tests, the polymerase chain reaction (PCR) method is considered as the 'gold standard' for the detection of some viruses and is characterized by rapid detection, high sensitivity, and specificity. As such, real-time reverse transcriptase-PCR (RT-PCR) is of great interest today for the detection of SARS-CoV -2 due to its benefits as a specific and simple qualitative assay [1][2][3]. Moreover, real-time RT-PCR has adequate sensitivity to help us much for diagnosing early infection.…”
mentioning
confidence: 99%