Real-time RT-PCR in COVID-19 detection: issues affecting the results

Tahamtan, Alireza; Ardebili, Abdollah

doi:10.1080/14737159.2020.1757437

Cited by 1,084 publications

(1,008 citation statements)

References 8 publications

(8 reference statements)

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“…To elaborate on these key issues, RT-PCR COVID-19 testing was shown to have high specificity with moderate sensitivity, resulting in cases of false negative results [21]. Usually, these false results are a consequence of incorrect sampling [22] and could be addressed by education of health care staff. Adequate sampling, good laboratory standards and high-quality RT-PCR kits, all reduce inaccurate results [22].…”

Section: Discussionmentioning

confidence: 99%

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Hazardous Postoperative Outcomes of Unexpected COVID‐19 Infected Patients: A Call for Global Consideration of Sampling all Asymptomatic Patients Before Surgical Treatment

et al. 2020

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Background In December 2019, a novel coronavirus was identified as the cause of many pneumonia cases in China and eventually declared as a pandemic as the virus spread globally. Few reports were published on the outcome of surgical procedures in diagnosed COVID-19 patients and even fewer on the surgical outcomes of asymptomatic undiagnosed COVID-19 surgical patients. We aimed to review all published data regarding surgical outcomes of preoperatively asymptomatic untested coronavirus disease 2019 (COVID-19) patients. Methods This report is a review on the perioperative period in COVID-19 patients who were preoperatively asymptomatic and not tested for COVID-19. Searches were conducted in PubMed April 4th, 2020. All publications, of any design, were considered for inclusion. Results Four reports were identified through our literature search, comprising 64 COVID-19 carriers, of them 51 were diagnosed only in the postoperative period. Synthesis of these reports, concerning the postoperative outcomes of patients diagnosed with COVID-19 during the perioperative period, suggested a 14/51 (27.5%) postoperative mortality rate and severe mostly pulmonic complications, as well as medical staff exposure and transmission. Conclusions COVID-19 may have potential hazardous implications on the perioperative course. Our review presents results of unacceptable mortality rate and a high rate of severe complications. These observations warrant further well-designed studies, yet we believe it is time for a global consideration of sampling all asymptomatic patients before surgical treatment.

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Section: Discussionmentioning

confidence: 99%

“…Usually, these false results are a consequence of incorrect sampling [22] and could be addressed by education of health care staff. Adequate sampling, good laboratory standards and high-quality RT-PCR kits, all reduce inaccurate results [22].…”

Section: Discussionmentioning

confidence: 99%

Hazardous Postoperative Outcomes of Unexpected COVID‐19 Infected Patients: A Call for Global Consideration of Sampling all Asymptomatic Patients Before Surgical Treatment

et al. 2020

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“…Uncertain negative results of RT-PCR regarding COVID-19 range from 22 to 30p100 (54,55,56), and RT-PCR results from several test at different points appear variable from the same patients during the course for diagnosis and treatment of patients (55).…”

Section: Discussionmentioning

confidence: 99%

Detection dogs as a help in the detection of COVID-19 Can the dog alert on COVID-19 positive persons by sniffing axillary sweat samples ? Proof-of-concept study

Grandjean

Sarkis²,

Tourtier³

et al. 2020

Preprint

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The aim of this study is to evaluate if the sweat produced by COVID-19 persons (SARS-CoV-2 PCR positive) has a different odour for trained detection dogs than the sweat produced by non COVID-19 persons. The study was conducted on 3 sites, following the same protocol procedures, and involved a total of 18 dogs. A total of 198 armpits sweat samples were obtained from different hospitals. For each involved dog, the acquisition of the specific odour of COVID-19 sweat samples required from one to four hours, with an amount of positive samples sniffing ranging from four to ten. For this proof of concept, we kept 8 dogs of the initial group (explosive detection dogs and colon cancer detection dogs), who performed a total of 368 trials, and will include the other dogs in our future studies as their adaptation to samples scenting takes more time.The percentages of success of the dogs to find the positive sample in a line containing several other negative samples or mocks (2 to 6) were 100p100 for 4 dogs, and respectively 83p100, 84p100, 90p100 and 94p100 for the others, all significantly different from the percentage of success that would be obtained by chance alone.We conclude that there is a very high evidence that the armpits sweat odour of COVID-19+ persons is different, and that dogs can detect a person infected by the SARS-CoV-2 virus.

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“…Currently, there is no effective vaccine or drug for preventing or treating COVID-19. Diagnosis of COVID-19 has been relying on molecular detection of the SARS-CoV-2 virus by RT-PCR or isothermal nucleic acid amplification methods in a narrow time window post infection [9][10][11][12] .…”

mentioning

confidence: 99%

High-Accuracy Multiplexed SARS-CoV-2 Antibody Assay with Avidity and Saliva Capability on a Nano-Plasmonic Platform

Liu¹,

Hsiung²,

Zhao³

et al. 2020

Preprint

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The outbreak and rapid spread of SARS-CoV-2 virus has led to a dire global pandemic with millions of people infected and ~ 400,000 deaths thus far. Highly accurate detection of antibodies for COVID-19 is an indispensable part of the effort to combat the pandemic 1,2 . Here we developed two-plex antibody detection against SARS-CoV-2 spike proteins 3 (the S1 subunit and receptor binding domain RBD) in human serum and saliva on a near-infrared nanoplasmonic gold (pGOLD) platform 4-8 . By testing nearly 600 serum samples, pGOLD COVID-19 assay achieved ~ 99.78 % specificity for detecting both IgG and IgM with 100 % sensitivity in sera collected > 14 days post disease symptom onset, with zero cross-reactivity to other diseases. Two-plex correlation analysis revealed higher binding of serum IgM to RBD than to S1. IgG antibody avidity toward multiple antigens were measured, shedding light on antibody maturation in COVID-19 patients and affording a powerful tool for differentiating recent from remote infections and identifying re-infection by SARS-CoV-2. Just as important, due to high analytical sensitivity, the pGOLD COVID-19 assay detected minute amounts of antibodies in human saliva, offering the first non-invasive detection of SARS-CoV-2 antibodies.

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Real-time RT-PCR in COVID-19 detection: issues affecting the results

Cited by 1,084 publications

References 8 publications

Hazardous Postoperative Outcomes of Unexpected COVID‐19 Infected Patients: A Call for Global Consideration of Sampling all Asymptomatic Patients Before Surgical Treatment

Hazardous Postoperative Outcomes of Unexpected COVID‐19 Infected Patients: A Call for Global Consideration of Sampling all Asymptomatic Patients Before Surgical Treatment

Detection dogs as a help in the detection of COVID-19 Can the dog alert on COVID-19 positive persons by sniffing axillary sweat samples ? Proof-of-concept study

High-Accuracy Multiplexed SARS-CoV-2 Antibody Assay with Avidity and Saliva Capability on a Nano-Plasmonic Platform

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