A Medium for the Production of Biopesticides (<I>Bacillus sphaericus</I> and <I>Bacillus thuringiensis</I> subsp. <I>israelensis</I>) in Mosquito Control

Abstract

Chicken feathers, discarded as bioorganic wastes from poultry processing industries, are rich in keratin (structural protein). Deoiled rice bran (DRB), in contrast, is an agrowaste product consisting of protein, fiber, and carbohydrates. This study highlights a new medium for producing biopesticides from these bioorganic wastes, by culturing Bacillus sphaericus (Bs) and B. thuringiensis subsp. israelensis (Bti), to synthesize mosquitocidal toxins. Biochemical studies indicated that the amount of mosquitocidal spore/crystal toxins produced from the experimental culture medium (chicken feather waste [CFW] + DRB) is higher than that from the conventional medium (Nutrient Yeast Extract Salt Medium [NYSM]). The bacteria produced in these media (NYSM, CFW, DRB, and CFW + DRB) were bioassayed against mosquito vectors, and the toxic effect was found to be statistically similar (fiducial limits overlapping). Cost analysis based on the raw materials, indicated that, the use of CFW and DRB, as culture medium, is highly economical, for the industrial production of these mosquito pathogenic bacilli.

Identification and Characterization of Mosquitocidal Toxins From <i>Bacillus cereus</i> VCRC-641

“…Crystal (Cry) and mosquitocidal (Mtx) toxins are the main types of toxins that Bti and B. sphaericus generates, and they have different compositions and synthesis times (Poopathi and Archana, 2012). Two polypeptides with molecular masses of roughly 51 and 42 kDa make up the B. sphaericus crystal toxin (Poopathi and Abidha, 2009;Charles and Nielsen-LeRoux, 2000). All these bacterial strains generate crystalline-endotoxin, which is only found in Coleoptera and Diptera (Hofte andWhitley, 1989, Poopathi andTyagi, 2002).…”

Toxicological studies for some agricultural waste extracts on mosquito larvae and experimental animals

“…No statistically significance was observed in the enzyme cholinesterase activity in treated mice as compared with control one. On the other hand, slight inhibition was observed after the 2 …”

“…These factors have created the need for environmental safe, degradable and target specific agents for pest control purposes. The use of deoiled rice bran and chicken feather waste, as culture medium, is highly economical, for the industrial production of the mosquito pathogenic bacillus [2] .…”

Microorganismes entomopathogènes, prédateurs et parasites des moustiques: Perspectives pour la lutte raisonnée contre les vecteurs du paludisme en Afrique sub-saharienne

“…Diverses méthodes de production et de formulation des bactéries entomopathogènes ont été explorées en vue d'améliorer leur coût et leur qualité (Xudong et al, 1993 ;Promdonkoy et al, 2004 ;Ohgushi et al, 2005 ;Park et al, 2005 ;Prabakaran et Balaraman, 2006 ;Ramirez-Suero et al, 2005 ;Sarrafzadeh et al, 2005 ;Federici et al, 2007 ;Prabakaran et al, 2007Prabakaran et al, , 2009Poopathi et Abidha, 2009).…”