A low-temperature-printed hierarchical porous sponge-like scaffold that promotes cell-material interaction and modulates paracrine activity of MSCs for vascularized bone regeneration

Lian, Meng; Sun, Binbin; Han, Yu; Yu, Bin; Xin, Weiwei; Xu, Ruida; Ni, Bing; Jiang, Wenbo; Hao, Yongqiang; Zhang, Xiuyin; Shen, Yi; Qiao, Zhiguang; Dai, Kerong

doi:10.1016/j.biomaterials.2021.120841

Cited by 66 publications

(56 citation statements)

References 67 publications

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“…Porous hydrogels can render macroscopic constructs with unique properties, receiving much attention as a promising biomaterial in 3D bioprinting. [35][36][37] With appropriate compositions and rational design, the printed porous hydrogels not only offer a favorable microenvironment for cell-ECM interactions but also enable the rapid exchange of substances to unlock the functions of the encapsulated cells, such as promoting the cell invasion, formation of new blood vessels, and cell viability. [35,38] However, few studies have proposed the methodology to fabricate pore-forming cellular constructs in situ by DLP-based bioprinting technology.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle‐Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs

Tao

Zhu

Zhou

et al. 2022

Adv Healthcare Materials

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A challenge for bioprinting tissue constructs is enabling the viability and functionality of encapsulated cells. Rationally designed bioink that can create appropriate biophysical cues shows great promise for overcoming such challenges. Here, a nanoparticle-stabilized emulsion bioink for direct fabrication of porous tissue constructs by digital light processing based 3D bioprinting technology is introduced. The emulsion bioink is integrated by the mixture of aqueous dextran microdroplets and gelatin methacryloyl solution and is further rendered stable by 𝜷-lactoglobulin nanoparticles. After bioprinting, the printed tissue constructs create the macroporous structure via removal of dextran, thereby providing favorable biophysical cues to promote the viability, proliferation, and spreading of the encapsulated cells. Moreover, a trachea-shaped construct containing chondrocytes is bioprinted and implanted in vivo. The results demonstrate that the generated macroporous construct is of benefit to cartilage tissue rebuilding. This work offers an advanced bioink for the fabrication of living tissue constructs by activating the cell behaviors and functions in situ and can lead to the development of 3D bioprinting.

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Section: Resultsmentioning

confidence: 99%

Nanoparticle‐Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs

Tao

Zhu

Zhou

et al. 2022

Adv Healthcare Materials

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“…Similarly, FAK signaling has been reported to mediate multiple cellular responses toward the physical cues provided by biomaterials [ 43 – 45 ]. Interestingly, previous studies have shown that focal adhesion kinase (FAK), downstream AKT signaling might participate in the required mechanotransductive pathways, through which the structures stimulated the paracrine function of MSCs [ 46 ]. Moreover, it has been reported that the mechanical effect could enhance the expression of adhesion molecule (FAK) and significantly increase the release of the factors VEGF and b-FGF from stem cells [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Mesh-like electrospun membrane loaded with atorvastatin facilitates cutaneous wound healing by promoting the paracrine function of mesenchymal stem cells

et al. 2022

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Background Functional electrospun membranes are promising dressings for promoting wound healing. However, their microstructure and drug loading capacity need further improvements. It is the first time to design a novel mesh-like electrospun fiber loaded with atorvastatin (ATV) and investigated its effects on paracrine secretion by bone marrow-derived mesenchymal stem cells (BMSCs) and wound healing in vivo. Methods We fabricated a mesh-like electrospun membrane using a copper mesh receiver. The physical properties of the membranes were evaluated by SEM, FTIR spectroscopy, tensile strength analysis, and contrast angle test. Drug release was measured by plotting concentration as a function of time. We tested the effects of conditioned media (CM) derived from BMSCs on endothelial cell migration and angiogenesis. We used these BMSCs and performed RT-PCR and ELISA to evaluate the expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) genes and proteins, respectively. The involvement of FAK and AKT mechanotransduction pathways in the regulation of BMSC secretion by material surface topography was also investigated. Furthermore, we established a rat model of wound healing, applied ATV-loaded mesh-like membranes (PCL/MAT) seeded with BMSCs on wounds, and assessed their efficacy for promoting wound healing. Results FTIR spectroscopy revealed successful ATV loading in PCL/MAT. Compared with random electrospun fibers (PCL/R) and mesh-like electrospun fibers without drug load (PCL/M), PCL/MAT induced maximum promotion of human umbilical vein endothelial cell (HUVEC) migration. In the PCL/MAT group, the cell sheet scratches were nearly closed after 24 h. However, the cell sheet scratches remained open in other treatments at the same time point. The PCL/MAT promoted angiogenesis and led to the generation of longer tubes than the other treatments. Finally, the PCL/MAT induced maximum gene expression and protein secretion of VEGF and b-FGF. As for material surface topography effect on BMSCs, FAK and AKT signaling pathways were shown to participate in the modulation of MSC morphology and its paracrine function. In vivo, PCL/MAT seeded with BMSCs significantly accelerated healing and improved neovascularization and collagen reconstruction in the wound area compared to the other treatments. Conclusions The mesh-like topography of fibrous scaffolds combined with ATV release creates a unique microenvironment that promotes paracrine secretion of BMSCs, thereby accelerating wound healing. Hence, drug-loaded mesh-like electrospun membranes may be highly efficacious for wound healing and as artificial skin. It is a promising approach to solve the traumatic skin defect and accelerate recovery, which is essential to developing functional materials for future regenerative medicine.

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“…These results might be due to the following reasons: (1) The porous structure of the microgels possessed a good nutrient and oxygen exchange efficiency, contributing to a favorable microenvironment for cell proliferation [ 4 ]. Moreover, appropriate pore size and roughness surface are more conducive to cell adherence and migration [ 49 ]. These relative small pore sizes offer a larger surface area and provide a certain ligand density that affects the integrin-binding after initial seeding.…”

Section: Discussionmentioning

confidence: 99%

Pearl-inspired graphene oxide-collagen microgel with multi-layer mineralization through microarray chips for bone defect repair

Zhou

Luo

Liu

et al. 2022

Materials Today Bio

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A low-temperature-printed hierarchical porous sponge-like scaffold that promotes cell-material interaction and modulates paracrine activity of MSCs for vascularized bone regeneration

Cited by 66 publications

References 67 publications

Nanoparticle‐Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs

Nanoparticle‐Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs

Mesh-like electrospun membrane loaded with atorvastatin facilitates cutaneous wound healing by promoting the paracrine function of mesenchymal stem cells

Pearl-inspired graphene oxide-collagen microgel with multi-layer mineralization through microarray chips for bone defect repair

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