Pearl-inspired graphene oxide-collagen microgel with multi-layer mineralization through microarray chips for bone defect repair

Zhou, Chuchao; Luo, Chao; Liu, Shaokai; Jiang, Shangxuan; Liu, Xin; Li, Jialun; Zhang, Xinyue; Wu, Xiaoyan; Sun, Jiaming; Wang, Zhenxing

doi:10.1016/j.mtbio.2022.100307

Cited by 10 publications

(7 citation statements)

References 64 publications

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“…The osteogenesis promoted by the GO-based collagen scaffold could be boosted by adding inorganics, such as strontium, to the mixture [ 89 ], activating a protein complex responsible for bone-cell proliferation and differentiation. GO-based collagen composites can also be coupled with hydroxyapatite, as reported by Zhou et al [ 90 ]. The authors crafted a multi-layer mineralized composite able to upregulate genes involved in osteogenesis, providing a comfortable microenvironment for hosting the cells of bone tissues.…”

Section: Carbon-containing Biopolymer Composites For Biomedical Appli...mentioning

confidence: 95%

A Short Review on Nanostructured Carbon Containing Biopolymer Derived Composites for Tissue Engineering Applications

2023

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The development of new scaffolds and materials for tissue engineering is a wide and open realm of material science. Among solutions, the use of biopolymers represents a particularly interesting area of study due to their great chemical complexity that enables creation of specific molecular architectures. However, biopolymers do not exhibit the properties required for direct application in tissue repair—such as mechanical and electrical properties—but they do show very attractive chemical functionalities which are difficult to produce through in vitro synthesis. The combination of biopolymers with nanostructured carbon fillers could represent a robust solution to enhance composite properties, producing composites with new and unique features, particularly relating to electronic conduction. In this paper, we provide a review of the field of carbonaceous nanostructure-containing biopolymer composites, limiting our investigation to tissue-engineering applications, and providing a complete overview of the recent and most outstanding achievements.

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Section: Carbon-containing Biopolymer Composites For Biomedical Appli...mentioning

confidence: 95%

A Short Review on Nanostructured Carbon Containing Biopolymer Derived Composites for Tissue Engineering Applications

2023

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“…To further validate the therapeutic efficacy of GO/Cu-functionalized hydrogel scaffolds in bone reconstruction, a modified calvarial bone defect model in rats was established according to previously published procedures [6,27,35]. The animal experiments reported herein were evaluated and approved by the Laboratory Animal Welfare and Ethical Committee (IACUC) of Central South University (Nos.…”

Section: In Vivo Bone Reconstruction Efficacy 271 Establishment Of a ...mentioning

confidence: 99%

“…The skull specimens (n = 3) containing ROIs as described in micro-CT scanning were processed for histological staining after samples were decalcified in 10% EDTA for approximately three to four weeks [6,36]. The routine procedures for paraffin embedment and preparation of tissue slices (4 μm in thickness) were similar to the abovementioned protocols, and selected slices at the mid-sagittal plane of the defect region were stained with H&E and Masson's trichrome to observe bone healing [36].…”

Section: Histological Evaluation and In Vivo Biosafetymentioning

confidence: 99%

“…To achieve satisfactory therapeutic outcomes, autologous, allogenic and artificial bone grafts have been extensively applied in current clinical scenarios [5]. Nevertheless, the clinical promotion and application of these grafts are always restricted due to donor site injury, limited availability, potential infectious disease transmission, immunological rejection and substantial economic burden [5][6][7]. As an alternative approach to satisfy the ever-increasing demand for bone repair, bone tissue engineering (BTE) has been a research hotspot for the last decade that has shown improvements in material science, processing techniques and in-depth understanding of bone healing mechanisms [5,8].…”

Section: Introductionmentioning

confidence: 99%

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Graphene oxide/copper nanosheet-integrated hydrogel platform as a bioactive and biocompatible scaffold to accelerate calvarial defect restoration

Yang,

Zhou,

et al. 2023

Preprint

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Background The treatment of craniofacial bone defects caused by trauma, tumors, and infectious and degenerative diseases is a significant issue in current clinical practice. Following the rapid development of bone tissue engineering (BTE) in the last decade, bioactive scaffolds coupled with multifunctional properties are in high demand with regard to effective therapy for bone defects. Methods In this study, motivated by the versatile biological functions of nanomaterials and synthetic hydrogels, copper nanoparticle (CuNP)-decorated graphene oxide (GO) nanosheets (GO/Cu) were combined with methacrylated gelatin (GelMA)-based organic-inorganic hybrids to construct porous bone scaffolds that mimic the extracellular matrix (ECM) of bone tissues by photocrosslinking. The material characterizations, in vitro cytocompatibility and osteogenesis of the biohybrid hydrogel scaffolds were investigated, and two different animal models were established to further confirm the in vivo neovascularization, macrophage recruitment, biocompatibility, biosafety and bone regenerative potential. Results It found that GO/Cu-functionalized GelMA/β-TCP hydrogel scaffolds exhibited evidently promoted osteogenic activities and excellent cytocompatibility, with favorable surface characteristics and sustainable release of Cu2+. Additionally, improved neovascularization, macrophage recruitment and tissue integration were found in mice implanted with the bioactive hydrogels. More importantly, the observations of microCT reconstruction and histological analysis in a calvarial bone defect model in rats treated with GO/Cu-incorporated hydrogel scaffolds, demonstrated significantly accelerated bone healing. Conclusions Taken together, this BTE-based bone repair strategy provides a promising and feasible method for constructing multifunctional GO/Cu nanocomposite- incorporated biohybrid hydrogel scaffolds with facilitated osteogenesis, angiogenesis and immunoregulation in one system, thereby demonstrating the great application potential for correcting craniofacial bone defects in future clinical scenarios.

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“…A. L. Oliveira et al demonstrated that dynamic conditions facilitated thicker apatite layers and higher mineralization efficiency compared to static conditions [28]. Altering the 3D scaffold structure also contributes to achieving uniform HAp distribution; Zhou C et al introduced a pearl-inspired microgel with multi-layer mineralization to achieve comprehensive mineralization [29].…”

Section: Introductionmentioning

confidence: 99%

3D-printed PCL framework assembling ECM-inspired multi-layer mineralized GO-Col-HAp microscaffold for in situ mandibular bone regeneration

Yang,

He,

Miao

et al. 2023

Preprint

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Background: In recent years, natural bone extracellular matrix (ECM)-inspired materials have found widespread application as scaffolds for bone tissue engineering. However, the challenge of creating scaffolds that mimic natural bone ECM's mechanical strength and hierarchical nano-micro-macro structures remains. The purposes of this study were to introduce an innovative bone ECM-inspired scaffold that integrates a 3D-printed framework with hydroxyapatite (HAp) mineralized graphene oxide-collagen (GO-Col) microscaffolds and find its application in the repair of mandibular bone defects. Methods: Initially, a 3D-printed PCL scaffold was designed with cubic disks and square pores to mimic the macrostructure of bone ECM. Subsequently, we developed multi-layer mineralized GO-Col-HAp microscaffolds (MLM GCH) to simulate natural bone ECM's nano- and microstructural features. Systematic in vitro and in vivo experiments were introduced to evaluate the ECM-inspired structure of the scaffold and to explore its effect on cell proliferation and its ability to repair rat bone defects. Results: The resultant MLM GCH/PCL composite scaffolds exhibited robust mechanical strength and ample assembly space. Moreover, the ECM-inspired MLM GCH microscaffolds displayed favorable attributes such as water absorption and retention and demonstrated promising cell adsorption and proliferation in vitro. The MLM GCH/PCL composite scaffolds exhibited successful bone regeneration within mandibular bone defects in vivo. Conclusions: This study presents a well-conceived strategy for fabricating ECM-inspired scaffolds by integrating 3D-printed PCL frameworks with multilayer mineralized porous microscaffolds, enhancing cell proliferation and bone regeneration. This construction approach holds the potential for extension to various other biomaterial types.

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Pearl-inspired graphene oxide-collagen microgel with multi-layer mineralization through microarray chips for bone defect repair

Cited by 10 publications

References 64 publications

A Short Review on Nanostructured Carbon Containing Biopolymer Derived Composites for Tissue Engineering Applications

A Short Review on Nanostructured Carbon Containing Biopolymer Derived Composites for Tissue Engineering Applications

Graphene oxide/copper nanosheet-integrated hydrogel platform as a bioactive and biocompatible scaffold to accelerate calvarial defect restoration

3D-printed PCL framework assembling ECM-inspired multi-layer mineralized GO-Col-HAp microscaffold for in situ mandibular bone regeneration

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