A longitudinal study of immune responses to <i>Plasmodium falciparum</i> sexual stage antigens in Tanzanian adults

Bousema, Teun; Drakeley, Chris; Kihonda, Japhet; Hendriks, Jan C.M.; Akim, N. I. J.; Roeffen, Will; Sauerwein, Robert W.

doi:10.1111/j.1365-3024.2007.00948.x

Cited by 58 publications

(77 citation statements)

References 37 publications

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“…Among children below 5 years of age, the prevalences of antibodies with specificity for NANP 6 (23.6%) and GLURP (32.4%) were broadly similar to those for Pfs48/45 (27.1%) and Pfs230 (38.1%). As expected, the prevalences of asexual stage antibodies to NANP 6 and GLURP increased significantly with age (adjusted OR ϭ 2.35, P Ͻ 0.001, and 95% CI of 1.98 to 2.78, and adjusted OR ϭ 1.79, P Ͻ 0.001, and 95% CI of 1.53 to 2.08, respectively), reflecting cumulative exposure to infection, while no evidence of an age-dependent increase in sexual stage-specific antibody responses was observed ( Table 2). The seroprevalences for Pfs48/45 and Pfs230 in the youngest children (27.1% and 38.1%, respectively) were comparable to those in adults (38% and 28.6%, respectively).…”

Section: Entomologysupporting

confidence: 82%

“…Thus, the prevalence of NANP 6 antibodies at the peak of the wet season was significantly higher than either at the start ( 2 ϭ 60.34; P Ͻ 0.001) or at the end ( 2 ϭ 48.91; P Ͻ 0.001) of the wet season. There was also a difference in NANP 6 antibody prevalence between the area of high transmission (60.9%) and the area of lower transmission (47.2%; 2 ϭ 12.61; P Ͻ 0.001). Prevalences of sexual stage-specific antibodies in relation to season.…”

Section: Entomologymentioning

confidence: 88%

“…Antibodies with specificity for NANP 6 were detected in 83.9% of individuals at the peak of the wet season, while this proportion was significantly lower at the start (44.2%) and at the end (48.5%) of the wet season. Thus, the prevalence of NANP 6 antibodies at the peak of the wet season was significantly higher than either at the start ( 2 ϭ 60.34; P Ͻ 0.001) or at the end ( 2 ϭ 48.91; P Ͻ 0.001) of the wet season. There was also a difference in NANP 6 antibody prevalence between the area of high transmission (60.9%) and the area of lower transmission (47.2%; 2 ϭ 12.61; P Ͻ 0.001).…”

Section: Entomologymentioning

confidence: 91%

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Naturally Acquired Immune Responses to Plasmodium falciparum Sexual Stage Antigens Pfs48/45 and Pfs230 in an Area of Seasonal Transmission

et al. 2011

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Acquisition of immunity to Plasmodium falciparum sexual stages is a key determinant for reducing humanmosquito transmission by preventing the fertilization and the development of the parasite in the mosquito midgut. Naturally acquired immunity against sexual stages may therefore form the basis for the development of transmission-blocking vaccines, but studies conducted to date offer little in the way of consistent findings. Here, we describe the acquisition of antigametocyte immune responses in malaria-exposed individuals in Burkina Faso. A total of 719 blood samples were collected in a series of three cross-sectional surveys at the start, peak, and end of the wet season. The seroprevalence of antibodies with specificity for the sexual stage antigens Pfs48/45 and Pfs230 was 2-fold lower (22 to 28%) than that for an asexual blood stage antigen glutamate-rich protein (GLURP) (65%) or for the preerythrocytic stage antigen circumsporozoite protein (CSP) (54%). The youngest children responded at frequencies similar to those for all four antigens but, in contrast with the immune responses to GLURP and CSP that increased with age independently of season and area of residence, there was no evidence for a clear age dependence of responses to Pfs48/45 and Pfs230. Anti-Pfs230 antibodies were most prevalent at the peak of the wet season (P < 0.001). Our findings suggest that naturally acquired immunity against Pfs48/45 and Pfs230 is a function of recent exposure rather than of cumulative exposure to gametocytes.

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Section: Entomologysupporting

confidence: 82%

Section: Entomologymentioning

confidence: 88%

Section: Entomologymentioning

confidence: 91%

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Naturally Acquired Immune Responses to Plasmodium falciparum Sexual Stage Antigens Pfs48/45 and Pfs230 in an Area of Seasonal Transmission

et al. 2011

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“…22,23 A more sensitive marker of gametocyte exposure than microscopy might be antibodies to two gametocyte antigens, Pfs230 and Pfs48/45. 37,38 Antibody responses to these molecules indeed appeared to be elevated in patent gametocyte carriers, suggesting that they may serve as specific markers of gametocyte exposure. Interestingly, in the current study, lower levels of antibodies to Pfs48/45 but not to Pfs230 were seen in subjects with schistosome infection.…”

Section: Discussionmentioning

confidence: 99%

Associations Between Helminth Infections, Plasmodium falciparum Parasite Carriage and Antibody Responses to Sexual and Asexual Stage Malarial Antigens

Ateba-Ngoa

Jones

Zinsou

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Infections with helminths and Plasmodium spp. overlap in their geographical distribution. It has been postulated that helminth infections may influence malarial transmission by altering Plasmodium falciparum gametocytogenesis. This cross-sectional study assessed the effect of helminth infections on P. falciparum gametocyte carriage and on humoral immune responses to sexual stage antigens in Gabon. Schistosoma haematobium and filarial infections as well as P. falciparum asexual forms and gametocyte carriage were determined. The antibody responses measured were to sexual (Pfs230, Pfs48/45) and asexual P. falciparum antigens (AMA1, MSP1, and GLURP). A total of 287 subjects were included. The prevalence of microscopically detectable P. falciparum asexual parasites was higher in S. haematobiuminfected subjects in comparison to their uninfected counterparts (47% versus 26%, P = 0.003), but this was not different when filarial infections were considered. Plasmodium falciparum gametocyte carriage was similar between Schistosomaor filaria-infected and uninfected subjects. We observed a significant decrease of Pfs48/45 immunoglobulin G titer in S. haematobium-infected subjects (P = 0.037), whereas no difference was seen for Pfs230 antibody titer, nor for antibodies to AMA1, MSP1, or GLURP. Our findings suggest an effect of S. haematobium on antibody responses to some P. falciparum gametocyte antigens that may have consequences for transmission-blocking immunity.

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“…In contrast, Pfs48/45 is expressed on gametocytes within the human host and is a target of naturally developed antibodies during infection, which may maintain greater antigenic diversity within parasite populations. 7,8 Understanding the diversity of these antigens can aid in rational vaccine design, especially for the design of straintranscendent vaccines. 9 Previously, we have used pooled amplicon deep sequencing to assess diversity of drug resistance alleles in parasite populations.…”

mentioning

confidence: 99%

Pooled Amplicon Deep Sequencing of Candidate Plasmodium falciparum Transmission-Blocking Vaccine Antigens

Juliano¹,

Parobek²,

Brazeau³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Polymorphisms within Plasmodium falciparum vaccine candidate antigens have the potential to compromise vaccine efficacy. Understanding the allele frequencies of polymorphisms in critical binding regions of antigens can help in the designing of strain-transcendent vaccines. Here, we adopt a pooled deep-sequencing approach, originally designed to study P. falciparum drug resistance mutations, to study the diversity of two leading transmission-blocking vaccine candidates, Pfs25 and Pfs48/45. We sequenced 329 P. falciparum field isolates from six different geographic regions. Pfs25 showed little diversity, with only one known polymorphism identified in the region associated with binding of transmission-blocking antibodies among our isolates. However, we identified four new mutations among eight non-synonymous mutations within the presumed antibody-binding region of Pfs48/45. Pooled deep sequencing provides a scalable and cost-effective approach for the targeted study of allele frequencies of P. falciparum candidate vaccine antigens.Malaria remains an important global public health threat with approximately 3.3 billion people living in regions with ongoing transmission. In 2013, estimates suggest that 198 million cases and 584,000 deaths were attributed to malaria.

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A longitudinal study of immune responses to Plasmodium falciparum sexual stage antigens in Tanzanian adults

Cited by 58 publications

References 37 publications

Naturally Acquired Immune Responses to Plasmodium falciparum Sexual Stage Antigens Pfs48/45 and Pfs230 in an Area of Seasonal Transmission

Naturally Acquired Immune Responses to Plasmodium falciparum Sexual Stage Antigens Pfs48/45 and Pfs230 in an Area of Seasonal Transmission

Associations Between Helminth Infections, Plasmodium falciparum Parasite Carriage and Antibody Responses to Sexual and Asexual Stage Malarial Antigens

Pooled Amplicon Deep Sequencing of Candidate Plasmodium falciparum Transmission-Blocking Vaccine Antigens

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