A long-term follow-up study of hyposensitization with immunoblotting

Jarolim, E.; Poulsen, Lars K.; Stadler, Beda M.; Mosbech, H.; Oesterballe, Ole; Kraft, Dietrich; Weeke, B.

doi:10.1016/0091-6749(90)90043-4

Cited by 59 publications

(44 citation statements)

References 33 publications

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“…For amplification of the genes for the human IgE heavy chain constant regions (IGHE) we used primers containing an AscI site (GAT CGG CGC GCC CAT CCG TCT TCC CCT TGA), an SfiI site, a 4xhis-tag (GAT CGG CCC AGC CGG CCT CAT TTA CCG GGA TTT ACA GAC AC), and for the C H 2-4 domains we used primers containing an AscI site (GAT CGG CGC GCC CAC CGT 4 Human genes: IGHG1 human IgG1 heavy chain constant region; IGHG4 human IgG4 heavy chain constant region; and IGHE, human IgE heavy chain constant region; FCER1A, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCER1G, Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide; MS4A2, membrane-spanning 4-domains, subfamily A, member 2 (Fc fragment of IgE, high affinity I, receptor for beta polypeptide).…”

Section: Amplification and Cloning Of Immunoglobulin Constant Regionsmentioning

confidence: 99%

“…Long-term exposure to higher concentrations of allergens or therapeutic intervention by specific immunotherapy (SIT) results in a T-helper cell type-1 shift in the immune response, leading to an increase in production of allergen-specific IgG antibodies, particularly of the IgG4 subclass. These IgG antibodies are thought to exert their function by blocking the IgE allergen interaction (2)(3)(4), by recruitment of Fc␥ receptors that inhibit FcR mediated activation (5,6 ), or by inhibition of IgE-facilitated allergen presentation to T cells (7 ).…”

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confidence: 99%

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Generation of Human Monoclonal Allergen-Specific IgE and IgG Antibodies from Synthetic Antibody Libraries

et al. 2007

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Background: Allergen-specific IgE and IgG antibodies play pivotal roles in the induction and progression of allergic hypersensitivity reactions. Consequently, monoclonal human IgE and IgG4 antibodies with defined specificity for allergens should be useful in allergy research and diagnostic tests. We used combinatorial antibody libraries and subsequent recombinant production to make and assess IgE, IgG1, and IgG4 allergen-specific antibodies. Methods: We used phage display to select a synthetic single-chain antibody fragment (scFv) library against 3 different allergens, from bee venom, bovine milk, and apple. The scFv obtained were converted into IgG1, IgG4, and IgE antibody formats and assessed for their biochemical properties by ELISA, immunoblotting, and fluorescence-activated cell sorting. Results: Two different antibody formats for each IgG1, IgG4, and IgE antibody were produced in mammalian cells as disulfide-linked and glycosylated Ig, which were usable in allergen-specific ELISA assays and immunoblots. In addition, the recombinant IgE antibodies mediated the binding of allergens to HEK-293 cells transfected with the high-affinity IgE receptor, and this binding was blocked by corresponding IgG antibodies. Conclusions: The use of synthetic libraries for the generation of allergen-specific recombinant IgE and IgG

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Section: Amplification and Cloning Of Immunoglobulin Constant Regionsmentioning

confidence: 99%

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confidence: 99%

Generation of Human Monoclonal Allergen-Specific IgE and IgG Antibodies from Synthetic Antibody Libraries

et al. 2007

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“…Reports of plasma cell dyscrasias [32][33][34] and vasculitis [35] after prolonged administration of allergens to promote allergic hyposensitization suggest that prolonged stimulation of the reticuloendothelial system may promote aberrant immune system behaviors in some humans. Such complications are apparently infrequent, however, as supported by failure to identify similar phenomena among populations of atopic patients receiving long-term hyposensitization therapy in allergy clinics [4,5].…”

Section: Discussionmentioning

confidence: 99%

“…examined, and to date, little in the way of long-term adverse health consequences have been documented [4,5]. Similar studies to determine the impact of repeated vaccination against bacterial, viral, or fungal pathogens have been infrequently performed.…”

Section: Introductionmentioning

confidence: 99%

Long-term health effects of repeated exposure to multiple vaccines

Pittman

Coonan

Gibbs

et al. 2004

Vaccine

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“…The theory of ‘blocking antibodies’ capable of inhibiting the IgE/allergen interaction is a long-known concept in the allergy field [1]. Immunotherapy with allergen extracts is associated with an increase of allergen-specific IgG, however, without correlation to the outcome of therapy [2]. This may be explained by the fact that immunizations with whole allergen molecules induce IgG that, depending on the epitope specificity, may enhance or inhibit the allergic reaction [3, 4].…”

Section: Introductionmentioning

confidence: 99%

IgE Mimotopes of Birch Pollen Allergen Bet v 1 Induce Blocking IgG in Mice

Ganglberger

Grünberger²,

Wiedermann³

et al. 2001

Int Arch Allergy Immunol

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Background: The induction of nonanaphylactogenic ‘blocking’ IgG antibodies capable of inhibiting the IgE/allergen interaction represents a favorable therapeutic concept for type I allergy. However, IgG antibodies to allergens may block or enhance specific IgE binding, depending on the recognized epitope. Taking the major birch pollen allergen Bet v 1 as a model, we developed a strategy for the precise induction of IgG antibodies of a desired epitope specificity. Methods: Random phage display peptide libraries were applied to define peptide structures mimicking natural epitopes (mimotopes) of Bet v 1. Selections were performed with BIP 1, a murine monoclonal antibody known to enhance the IgE binding to Bet v 1, and with anti-Bet v 1 IgE purified from patients’ sera. The characterized Bet v 1 mimotopes were used to localize the corresponding epitope at the surface of Bet v 1 by a computer-aided mathematical approach based on the three-dimensional structure and the chemical character of the amino acids. The Bet v 1 mimotopes were further used to immunize BALB/c mice. The specificity of the induced antibodies was tested by immunoblotting and inhibition assays. Results: With the three-dimensional epitope search it became possible to localize a discontinuous IgE epitope on the surface of Bet v 1 in a substantial distance from the IgG epitope of the monoclonal antibody BIP 1. Moreover, we could demonstrate that phage displaying mimotopes are immunogenic vectors for the precise induction of epitope-specific IgG. Immunization with BIP 1 mimotopes induced IgG enhancing the IgE binding to Bet v 1, whereas immunization with IgE mimotopes resulted in IgG capable of blocking human IgE binding in vitro. Conclusion: Allergen mimotopes can be used for the induction of anti allergen IgG of desired specificity. We propose that mimotope immunotherapy based on IgE mimotopes generated by biopannings may represent a future concept for therapy of type I allergy.

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A long-term follow-up study of hyposensitization with immunoblotting

Cited by 59 publications

References 33 publications

Generation of Human Monoclonal Allergen-Specific IgE and IgG Antibodies from Synthetic Antibody Libraries

Generation of Human Monoclonal Allergen-Specific IgE and IgG Antibodies from Synthetic Antibody Libraries

Long-term health effects of repeated exposure to multiple vaccines

IgE Mimotopes of Birch Pollen Allergen Bet v 1 Induce Blocking IgG in Mice

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