A liquid chromatography–tandem mass spectrometry method for the detection of economically motivated adulteration in protein-containing foods

MacMahon, Shaun; Begley, Timothy H.; Diachenko, Gregory W.; Stromgren, Selen A.

doi:10.1016/j.chroma.2011.11.066

Cited by 90 publications

(43 citation statements)

References 11 publications

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“…In the event of fraudulent practice in the dairy industry, adequate control methods are required to evaluate the authenticity of milk and milk products. Methods involving application of electrical admittance spectroscopy (Sadat, Mustajab, & Khan, 2006), single frequency conductance measurements (Mabrook & Petty, 2003), digital image (Santos, Wentzell, & Pereira-Filho, 2011), chromatography (MacMahon, Begley, Diachenko, & Stromgren, 2012), ultraviolet-visible light spectroscopy (Hsieh, Hung, & Kuo, 2011), enzyme-linked immunosorbent assay (Asensio, Gonzalez, Garcıa, & Martın, 2008) and others (Recio, Garcia-Risco, Lopez-Fandino, Olano, & Ramos, 2000) have been applied to detect milk adulteration.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection and quantification of milk adulteration using infrared microspectroscopy and chemometrics analysis

2013

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Section: Introductionmentioning

confidence: 99%

Rapid detection and quantification of milk adulteration using infrared microspectroscopy and chemometrics analysis

2013

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“…Consequently, there is a demand for an analytical technique that can reliably monitor for DCD in powdered milk. The common methods of detection for DCD involve HPLC-UV [21], HPLC-MS/MS [22], ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) [23], and ion exclusion chromatography (IEC-UV) [24]. Although current mass spectrometric methods are very powerful, they involve multiple sample preparation steps (extraction, derivatization, etc.)…”

mentioning

confidence: 99%

Determination of Dicyandiamide in Powdered Milk Using Direct Analysis in Real Time Quadrupole Time-of-Flight Tandem Mass Spectrometry

Zhang

Yong

Liu

et al. 2015

J. Am. Soc. Mass Spectrom.

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MS DART DCDAbstract. The direct analysis in real time (DART) ionization source coupled with quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) system has the capability to desorb analytes directly from samples without sample cleanup or chromatographic separation. In this work, a method based on DART/Q-TOF MS/MS has been developed for rapid identification of dicyandiamide (DCD) present in powdered milk. Simple sample extraction procedure employing acetonitrile-water (80:20, v/v) mixture was followed by direct, high-throughput determination of sample extracts spread on a steel mesh of the transmission module by mass spectrometry under ambient conditions. The method has been evaluated for both qualitative and quantitative analysis of DCD in powdered milk. Variables including experimental apparatus, DART gas heater temperature, sample presentation speed, and vacuum pressure were investigated. The quantitative method was validated with respect to linearity, sensitivity, repeatability, precision, and accuracy by using external standards. After optimization of these parameters, a limit of detection (LOD) of 100 μg kg -1 was obtained for DCD with a linear working range from 100 to 10000 μg kg -1 and a satisfactory correlation coefficient (R 2 ) of 0.9997. Good recovery (80.08%-106.47%) and repeatability (RSD=3.0%-5.4%) were achieved for DCD. The DART/Q-TOF MS/MS-based method provides a rapid, efficient, and powerful scheme to analyze DCD in powdered milk with limited sample preparation, thus reducing time and complexity of quality control.

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“…The deliberate adulteration of milk with specific high nitrogencontent compounds could be carried out to increase the apparent nitrogen level and thus the value of the milk [1]. Such adulteration creates food safety, ethical and legal implications.…”

Section: Introductionmentioning

confidence: 98%

Lactose semicarbazone as a marker for semicarbazide adulteration in milk

Abernethy¹,

Higgs²

2013

Journal of Chromatography A

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A liquid chromatography–tandem mass spectrometry method for the detection of economically motivated adulteration in protein-containing foods

Cited by 90 publications

References 11 publications

Rapid detection and quantification of milk adulteration using infrared microspectroscopy and chemometrics analysis

Rapid detection and quantification of milk adulteration using infrared microspectroscopy and chemometrics analysis

Determination of Dicyandiamide in Powdered Milk Using Direct Analysis in Real Time Quadrupole Time-of-Flight Tandem Mass Spectrometry

Lactose semicarbazone as a marker for semicarbazide adulteration in milk

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