Lactose semicarbazone as a marker for semicarbazide adulteration in milk

Abernethy, Grant; Higgs, Kerianne

doi:10.1016/j.chroma.2013.03.075

Cited by 14 publications

(8 citation statements)

References 3 publications

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“…Under optimized performing conditions, a detection limit of 1 mmol/L was achieved. Though at this stage the sensitivity of the method proposed here is lower than that obtained from chromatography-mass spectrum techniques, [12][13][14]16,18 it has a few visible advantages: 1) no requirement of expensive instruments; 2) rapid response; 3) easy operation; 4) free of harmful auxiliary reagents. In particular, it will provide an opportunity to establish a method for detection SEM and nitrofurazone simultaneously, whose electroactive character has been demonstrated previously.…”

Section: Discussionmentioning

confidence: 99%

“…All these results indicate that the precision, reproducibility and stability of this detection method are satisfactory, though its sensitivity needs to be improved further in comparison with some of other methods. [12][13][14]16,18 The possible interference of foreign species on the detection of SEM was tested at the GME. In 1.0 mol/L acetate buffer solution of pH 4.0 containing 40 mmol/L SEM, several foreign molecules were added, respectively.…”

Section: Voltammetric Detection Methodsmentioning

confidence: 99%

“…From the data in the table we can see: 1) almost all the metallic ions have no visible influence on the quantitative detection of SEM; 2) low concentration of simple organic molecules also have no visible influence on the detection; 3) some big molecules, those contain benzenoid structure or p electron structure in particular, affect the performance remarkably, maybe owing to the competitive adsorption, for most of those molecules display adsorption features at carbon electrodes; 32,36,37 4) aldehyde or reducing sugar will decrease the sensitivity remarkable owing to the ready reactions between SEM and them. 12 In addition, the influence of SEM's precursor molecule, i.e. nitrofurazone, on the voltammetric detection, was investigated in particular.…”

Section: Voltammetric Detection Methodsmentioning

confidence: 99%

“…To the best of our knowledge, there are no reports on the simultaneous detection of SEM and nitrofurazone based on chromatography-mass spectrum techniques. [12][13][14][15][16][17][18] Here the potential electrochemical method is reasonable to pave a promising way for distinguishing whether the presence of SEM is resulted from nitrofurazone or not.…”

Section: Voltammetric Detection Methodsmentioning

confidence: 99%

“…11 As a result, the discovery of SEM in food arising from various sources has caused the argument on the validity of using SEM as the marker metabolite for indicating illegal nitrofurazone treatment. 6,8 3) SEM is a potential candidate for use in milk adulteration because it is a small, water-soluble and colourless compound with a relatively high nitrogen content of 56%, 12 Based on the contents mentioned above, it can be realized undoubtedly that more efforts and energy will be put on the investigation of SEM in the close future. Consequently, fine detection methods, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Voltammetric Behavior of Semicarbazide at Graphene Modified Electrode and Application to Detection

Zhang

et al. 2014

J Chinese Chemical Soc

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Herein voltammetric behaviors of semicarbazide (SEM) are investigated by employing a graphene modified working electrode, which displays attractive electroanalytical properties. In the acetate buffer solution of pH 4.00, there is a well-defined oxidative peak of SEM, attributing to the irreversible and adsorption-controlled electrode reaction with 2 electrons participation. The values of apparent heterogeneous electron transfer rate constant k¢ s with the redox are 0.0061 s -1 and 0.0009 s -1 , respectively, for two scan rate section. After the experimental parameters, which influence the voltammetric responses of SEM, including supporting electrolyte, pH, accumulation time and accumulation potential, etc., are optimized, it is found that the anodic peak current of SEM is proportional to its concentration in the range of 4 -40 mmol/L with a detection limit of 1 mmol/L (S/N = 3). Then, an electrochemical method for detecting SEM quantitatively is developed successfully. The concentrations of SEM in fortified tap water samples are tested with satisfactory recovery, indicating that the novel method is strongly promising in the environmental monitoring application.

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Section: Discussionmentioning

confidence: 99%