A liquid chromatography assay for a quantification of doripenem, ertapenem, imipenem, meropenem concentrations in human plasma: Application to a clinical pharmacokinetic study

Dailly, Éric; Bouquié, Regis; Deslandes, Guillaume; Jolliet, Pascale; Floch, R. Le

doi:10.1016/j.jchromb.2011.03.038

Cited by 62 publications

(61 citation statements)

References 31 publications

(36 reference statements)

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“…Stock solutions of each beta-lactam were prepared at 50 or 100 mg/ml in water and stored at Ϫ80°C. Under these conditions they are stable for a few months (9,18,25). They were further mixed in water to obtain a 1-mg/ml solution, which was used to produce standard solutions at 250, 125, 40, and 10 g/ml.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Determination of 12 β-Lactam Antibiotics in Human Plasma by High-Performance Liquid Chromatography with UV Detection: Application to Therapeutic Drug Monitoring

Verdier

Tribut

Tattevin

et al. 2011

Antimicrob Agents Chemother

128

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A rapid and specific high-performance liquid chromatography method with UV detection (HPLC-UV) for the simultaneous determination of 12 beta-lactam antibiotics (amoxicillin, cefepime, cefotaxime, ceftazidime, ceftriaxone, cloxacillin, imipenem, meropenem, oxacillin, penicillin G, piperacillin, and ticarcillin) in small samples of human plasma is described. Extraction consisted of protein precipitation by acetonitrile. An Atlantis T3 analytical column with a linear gradient of acetonitrile and a pH 2 phosphoric acid solution was used for separation. Wavelength photodiode array detection was set either at 210 nm, 230 nm, or 298 nm according to the compound. This method is accurate and reproducible (coefficient of variation [CV] < 8%), allowing quantification of beta-lactam plasma levels from 5 to 250 g/ml without interference with other common drugs. This technique is easy to use in routine therapeutic drug monitoring of beta-lactam antibiotics.

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Section: Methodsmentioning

confidence: 99%

“…There are several proposed methods for assaying beta-lactams in biological fluids, either for a particular compound (5,11,24,30) or for a group of compounds (9,10,13,17,18,21,29,33,34). Many other methods were developed for food industry and agriculture applications (1).…”

mentioning

confidence: 99%

Simultaneous Determination of 12 β-Lactam Antibiotics in Human Plasma by High-Performance Liquid Chromatography with UV Detection: Application to Therapeutic Drug Monitoring

Verdier

Tribut

Tattevin

et al. 2011

Antimicrob Agents Chemother

128

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“…8 Several methods of liquid chromatography coupled to either UV absorbance or mass spectrometry detection have been developed for the quantification of ertapenem in human plasma. [9][10][11][12][13] A LC-MS method was developed for the quantification of ERP in human plasma by detecting the deprotonated molecular ion of ERP under negative ionization mode with lower limit of quantification of 0.5 or 1 µg/ml and analytical run time of more than 5 min. 14 Chromatography.…”

Section: 2mentioning

confidence: 99%

Development of Stability Indicating RP-HPLC Method for Ertapenem in Bulk Drug and Pharmaceutical Dosage Form

Jain

Jain²,

Jain

et al. 2017

Dhaka Univ. J. Pharm. Sci

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ABSTRACT:A simple, inexpensive, rapid and novel stability indicating isocratic HPLC method has been developed and validated for quantitative analysis of ertapenem sodium in the bulk drug and in pharmaceutical dosage form. An isocratic separation of ertapenem sodium was achieved on Hypersil BDS C 18 column (4.6 x 250 mm, 5 µ particle size) as the stationary phase with a flow rate of 1.2 ml/min and using a UV detector to monitor the eluate at 298 nm. The mobile phase consisted of acetonitrile : water (60:40v/v) and pH adjusted 2.9 by othophosphoric acid enabled separation of the drug from its degradation products. The method was validated for linearity, accuracy (recovery), precision, specificity and robustness. The linearity of the method was satisfactory over the range 2-10 µg/ml (correlation coefficient 0.999). Recovery of ertapenem sodium from the pharmaceutical dosage form ranged from 99.97 to 103.7%. Ertapenem sodium was subjected to stress conditions [hydrolysis (acid, base), oxidation, photolysis and thermal degradation] and the samples were analyzed by this method. The forceddegradation study with ertapenem sodium showed that it was degraded under basic condition. The drug was stable under the other stress conditions investigated. Ertapenem sodium was found to be less stable in solution state, whereas it was comparatively much stable in solid state. The degradation products were well resolved from main peak. The forced degradation study prove the stability indicating power of the method and therefore, the validated method may be useful for routine analysis of ertapenem sodium as bulk drug, in respective dosage forms, for dissolution studies and as stability indicating assay method in pharmaceutical laboratories and industries.

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“…DP and MP were broad spectrum of antimicrobial activity against both gram-positive and gram-negative bacteria, including pseudomonas aeruginosa [3][4][5].The catalytic effect of citrate, phosphate and acetate buffers on the degradation of imipenem has been studied [6]. Several methods have been reported in the literature for the determination of doripenem and meropenem and its metabolites in biological fluids and pharmaceutical formulation using different techniques, including spectrophotometry, high performance liquid chromatography (HPLC) and liquid chromatography methods [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Graphene and Polyaniline Composite Modified Glassy Carbon Electrode for Electrochemical Determination of Doripenem and Meropenem Metabolites

Sivaprasad¹

2014

J Anal Bioanal Tech

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Harrison et al. [22] studied the metabolism of meropenem and *Corresponding author: Sreedhar NY, Electroanalytical Lab, Department of Chemistry, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India, Tel: +91-9440087124; E-mail: sreedharny.chem@gmail.com AbstractThe theme of this study was the preparation, characterization, and application of a polyaniline (PAN) and graphene composite modified glassy carbon electrode (PAN/Gr/GCE) for the voltammetric determination of doripenem (DPM) and meropenem metabolites (MPM) in human urine and serum samples. The morphological study of the PAN/ Gr/GCE composite was examined by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrochemical behaviour of DPM and MPM at the PAN/Gr/GCE were investigated using cyclic voltammetry in Ag/ AgCl/KCl supporting electrolyte at pH 2.0-10.0 in phosphate buffer solution. The linear dependence of current versus concentration was reached in a wide concentration range from 2.5×10 -7 M to 3.5×10 -4 M using cyclic voltammetry and differential pulse voltammetric methods. In acidic media a non-reversible diffusion controlled reduction involving two protons and two electrons occurs at carbon and nitrogen double bond (C=N) in the metabolites. The best electroanalytical performances of this composite electrode were achieved with the detection limits 3.6×10 -9 M and 1.75×10 -9 M for DPM and MPM respectively. The simplicity of preparation, high sensitivity and stability of this composite electrode should open novel avenues and applications for fabricating robust sensors for detection of DPM and MPM in human urine and serum samples.

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A liquid chromatography assay for a quantification of doripenem, ertapenem, imipenem, meropenem concentrations in human plasma: Application to a clinical pharmacokinetic study

Cited by 62 publications

References 31 publications

Simultaneous Determination of 12 β-Lactam Antibiotics in Human Plasma by High-Performance Liquid Chromatography with UV Detection: Application to Therapeutic Drug Monitoring

Simultaneous Determination of 12 β-Lactam Antibiotics in Human Plasma by High-Performance Liquid Chromatography with UV Detection: Application to Therapeutic Drug Monitoring

Development of Stability Indicating RP-HPLC Method for Ertapenem in Bulk Drug and Pharmaceutical Dosage Form

Graphene and Polyaniline Composite Modified Glassy Carbon Electrode for Electrochemical Determination of Doripenem and Meropenem Metabolites

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