A light and electron microscopic study of the early stages of root surface formation in molar teeth in the rat

Owens, Phasefanie

doi:10.1016/0003-9969(79)90215-2

Cited by 72 publications

(43 citation statements)

References 11 publications

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“…Recently, OWENS (1980), LINDSKOG (1982), andTEN CATE (1985) reported that the ILC was probably formed by the inner enamel epithelial cells of Hertwig's epithelial root sheath, not by the cementoblasts.…”

Section: Discussionmentioning

confidence: 99%

The innermost layer of cementum in rat molars : Its ultrastructure, development, and calcification.

Yamamoto

1986

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Discussionmentioning

confidence: 99%

The innermost layer of cementum in rat molars : Its ultrastructure, development, and calcification.

Yamamoto

1986

Arch. Histol. Jap., Arch. Histol. Jpn

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“…The MPA-positive reaction in the outermost layer of root dentin suggested characteristic components of glycoconjugates compared to other parts of dentin. This inter pretation may correspond to the outermost nonmineralized dentinal matrix described by Owens [22]. Forming cellular ccmentum and alveolar bone stained positive with MPA.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Glycoconjugates Localized by Peanut and <i>Madura pomifera</i> Agglutinins during Mouse Molar Root Development

Sasano

Kaji²,

Nakamura³

et al. 1992

Cells Tissues Organs

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Glycolipids, glycoproteins, glycosaminoglycans and sialoglycoproteins have all been implicated in a number of developmentally significant processes related to complex interactions between cell surfaces and the extracellular matrix. The present study was designed to localize glycoconjugates recognized by peanut agglutinin (PNA) and Madura pomifera (MPA) lectins during mouse molar root development. Postnatal ICR mice at 10, 15, 21, 28 and 42 days were used. Lower jaws were dissected, fixed in 4% paraformaldehyde, decalcified in 5% EDTA and embedded in paraffin. Serial sections were made and stained with FITC-conjugated PNA or MPA. β-Lactose was used as an inhibitory sugar for PNA, and α-D-melibiose for MPA. PNA specifically stained Hertwig’s epithelial root sheath (HERS), whereas MPA stained a number of tissues. The outermost layer of root dentin, forming cellular cementum, alveolar bone and HERS showed positive reactions with MPA. Glycoconjugates localized by the lectins may be functionally related to molecules which contribute to root formation and cementogenesis.

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“…However, there have been different opinions: that the initial cementum is an epithelial secretory product (Paynter and Pudy 1958;Owens 1980;Slavkin et al 1989;Hammarstöm et al 1996;Hammarstöm 1997);…”

Section: The Mineralization Of Acellular Cementummentioning

confidence: 99%

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis(2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP-and OPN-immunoreactive, initial cementum layer appeared. At this point cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: During the initial cementogenesis cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment mineralization advances along collagen fibrils without matrix vesicles.

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A light and electron microscopic study of the early stages of root surface formation in molar teeth in the rat

Cited by 72 publications

References 11 publications

The innermost layer of cementum in rat molars : Its ultrastructure, development, and calcification.

The innermost layer of cementum in rat molars : Its ultrastructure, development, and calcification.

Distribution of Glycoconjugates Localized by Peanut and <i>Madura pomifera</i> Agglutinins during Mouse Molar Root Development

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

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