The innermost layer of cementum in rat molars : Its ultrastructure, development, and calcification.

Yamamoto, Tsuneyuki

doi:10.1679/aohc.49.459

Cited by 33 publications

(25 citation statements)

References 29 publications

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“…The study here found the localization and size of GBHA reactive particles very similar to those of calciferous spherules observed in a previous study (Yamamoto 1986). This suggests that the particles correspond to the calciferous spherules.…”

Section: The Mineralization Of Acellular Cementumsupporting

confidence: 89%

“…We have studied the structure and development of the acellular cementum in rat molars by light and electron microscopy (Yamamoto 1986;Yamamoto and Wakita 1990;Yamamoto et al 2000aYamamoto et al , 2000bYamamoto et al , 2001Yamamoto et al , 2004Yamamoto et al , 2005, and found the following: (1) the cemento-dentinal junction is a fibril-poor layer (1-2 μm thick) and contains more densely accumulated BSP and OPN than the successively formed acellular cementum with extrinsic fibers; (2) developmentally, prior to the principal fiber attachment to the root surface, BSP and OPN are densely deposited as the initial cementum, which results in the fibril-poor cemento-dentinal junction; (3) during the initial cementogenesis, matrix vesicles and calciferous spherules appear on the root surface.…”

Section: The Mineralization Of Acellular Cementummentioning

confidence: 99%

“…For rat molars, it is generally accepted that the cemento-dentinal junction is fibril-poor and contains more highly accumulated BSP and OPN than other parts of cementum (Paynter and Pudy 1958;Yamamoto 1986;Yamamoto and Wakita 1990;Yamamoto et al 2000aYamamoto et al , 2000bYamamoto et al , 2001Yamamoto et al , 2004Yamamoto et al , 2005Sasano et al 2001;Tadatomo et al 2002;Hosoya et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis(2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP-and OPN-immunoreactive, initial cementum layer appeared. At this point cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: During the initial cementogenesis cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment mineralization advances along collagen fibrils without matrix vesicles.

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Section: The Mineralization Of Acellular Cementumsupporting

confidence: 89%

Section: The Mineralization Of Acellular Cementummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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“…Bars 3 µm (a, b) that this mode of cell death is not predominant during root formation. It is generally accepted that HERS and ERM cells contain poorly developed rER, a few lysosomes, and relatively well developed mitochondria and tonofilaments and that these cells connect with one another forming desmosomes and gap junctions (Lester 1969;Yamamoto 1986;Hamamoto et al 1989). Therefore, we suggest that electron-lucent cells are dying HERS cells based on the cytoplasmic contents and desmosomes.…”

Section: Discussionmentioning

confidence: 99%

Cell proliferation and death of Hertwig's epithelial root sheath in the rat

Kaneko

Hashimoto

Enokiya

et al. 1999

Cell and Tissue Research

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Hertwig's epithelial root sheath (HERS) degenerates immediately after root dentin is formed. However, odontogenic tumors or cysts may originate from residual cells, although little is known about how HERS proliferates and disappears. This study investigated whether cell death is provoked in the tissues surrounding the root during eruption of the rat upper molar. We employed the TdT-mediated-dUTP nick end labeling (TUNEL) method and transmission electron microscopy (TEM) to observe the morphological features of cell death. We examined the activity of cell proliferation immunohistochemically using proliferative cell nuclear antigen (PCNA) and the continuity of HERS using polyclonal keratin antibody (PK). Cell death resembling apoptosis and apoptotic bodies phagocytosed by neighboring mesenchymal cells were detected in only a few cells by both TUNEL and TEM. We also found cells with electron-lucent cytoplasm which contained dilated or ruptured mitochondria and remarkably dilated rough endoplasmic reticulum (rER) which lay sparsely along the root. These cells seemed to be dead HERS cells based on their ultrastructural features, location, and stage. PCNA-positive cells were found in the apical end of the HERS cells, fibroblasts of the periodontal ligament, and odontoblasts. PK reacted with HERS; however, PK-positive cells partially disappeared after the 15th postnatal day when the root dentin had formed slightly. These results may indicate that HERS cells migrate into the periodontal ligament or die immediately after root dentin is formed and that various types of cell death such as apoptosis and cytoplasmic type occur in the tissues surrounding the root during tooth development.

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“…These proteins may mediate root and/or cementum formation, and do not appear to become major constituents of cementum (Luan et al, 2006). We think it likely that major interfibrillar matrices such as bone sialoprotein and osteopontin are products of dental follicle-derived cementoblasts in normal cementogenesis (Yamamoto, 1986;Cho and Garant, 1988Diekwisch, 2001;Yamamoto and Wakita, 1990;Yamamoto et al, 2004Yamamoto et al, , 2007Hirata and …”

Section: Runx2-keratin Double Stainingmentioning

confidence: 98%

Hertwig's epithelial root sheath cells do not transform into cementoblasts in rat molar cementogenesis

Yamamoto

Takahashi

2009

Annals of Anatomy - Anatomischer Anzeiger

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The innermost layer of cementum in rat molars : Its ultrastructure, development, and calcification.

Cited by 33 publications

References 29 publications

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Cell proliferation and death of Hertwig's epithelial root sheath in the rat

Hertwig's epithelial root sheath cells do not transform into cementoblasts in rat molar cementogenesis

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