A label-free silver wire based impedimetric immunosensor for detection of aflatoxin M1 in milk

Bacher, Gautam; Pal, Souvik; Kanungo, Lizy; Bhand, Sunil

doi:10.1016/j.snb.2012.04.012

Cited by 83 publications

(23 citation statements)

References 36 publications

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“…Based on the results obtained, the designed nanobiosensor showed more sensitivity (10 -12 mol aflatoxin B1) compared to our previous study (REF). By reviewing other similar studies, we found out that the present nanobiosensor possessed the highest sensitivity, very close to the sensitivity of the impedimetric immunosensor designed by Bacher et al 2012. Water-soluble, highly monodispersed and stable Cd/Te QDs and magnetic/silica core shell were synthesized to fabricate the nanobiosensor.…”

Section: Discussionsupporting

confidence: 59%

Highly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier

et al. 2015

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Section: Discussionsupporting

confidence: 59%

Highly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier

et al. 2015

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“…20 Further, recent studies have resulted in publication of a several biosensor-based methods for AFM1 determination in milk samples. 21,22 ELISA is defined as routine screening method which may be performed with a great number of commercially available test kits (Neogen Veratox®, Lansing, USA; Tecna S. r. l., Trieste, Italy; Ridascreen, R-Biopharm, Darmstad, Germany; Immunolab GmbH, Kassel, Germany; etc.). The major advantages of ELISA method are minimal sample clean-up and preparation, simple measurement procedure and low cost.…”

Section: Kos Et Al: Comparison Of Elisa Hplc-fld and Hplc-ms/ms mentioning

confidence: 99%

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Kos

Hajnal²,

Jajić³

et al. 2016

ACSi

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Presence of aflatoxin M1 (AFM1) in milk should be continuously controlled in order to protect the population from risks associated with its proven toxicity and carcinogenicity. During recent years, there has been an increase in demand for development of sensitive, accurate, simple and fast method which is reliable for detection of AFM1 at low concentrations found in milk samples. For that purpose, enzyme linked immunosorbent asssay (ELISA), high performance liquid chromatography with fluorescence detector (HPLC-FLD) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were optimized and validated in order to apply them for AFM1 analysis in naturally contaminated milk samples, and to assess the closeness of agreement between results of three different methods. The obtained validation parameters indicate that all three methods are suitable for determination of AFM1 in milk samples. The statistical analysis of variance between the methods and the obtained correlation coefficients indicate that there is a strong correlation between methods. All three methods are satisfactory in meeting the requirements for official control purposes. To the best of author's knowledge, this study represents the first report of an investigation and comparison of ELISA, HPLC-FLD and HPLC-MS/MS methods for determination of AFM1 in naturally contaminated milk samples.

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“…8,14,15 However, the quantitative approaches require complicated pretreatment, professional operators and expensive instruments. Some immunological methods like enzyme-linked immune sorbent assay (ELISA) [16][17][18] and immunosensors [19][20][21] have also been reported for AFM 1 detection. There are disadvantages in both preparation of the antibody and its stability and this limits its application in the field.…”

Section: Introductionmentioning

confidence: 99%

A qPCR aptasensor for sensitive detection of aflatoxin M1

et al. 2016

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. In addition, the aptasensor developed here exhibits high selectivity for AFM 1 over other mycotoxins and small effects from cross-reaction with structural analogs. The method proposed here has been successfully applied to quantitative determination of AFM 1 in infant rice cereal and infant milk powder samples. Results demonstrated that the current approach is potentially useful for food safety analysis, and it could be extended to a large number of targets. IntroductionAflatoxin M 1 (AFM 1 ), one of the most toxic contaminants in dairy products, is a metabolite produced by dairy cows as a result of being fed with feeds contaminated with Aflatoxin B 1 (AFB 1 ). [3][4][5] Once present in dairy products, AFM 1 poses a hazard to humans (especially infants) who consume them. Results and Discussion Optimization of the amplification of complementary ssDNAThe complementary ssDNA is applied as the subsequent PCR template. The Optimization of streptavidin and the biotinylated aptamerOther factors would affect the performance of this method including the concentrations of streptavidin, the biotinylated aptamer and the complementary ssDNA and these all should be optimized. Under a fixed concentration of complementary ssDNA at 10 nM, the concentrations of streptavidin and the 6 biotinylated aptamer were analyzed for the PCR amplification signal change (Fig. S3).The adsorptive power of streptavidin to PCR tubes and the binding ability of streptavidin-coated tubes to biotinylated aptamer was primarily detected using different concentrations of streptavidin (Fig. S3). This also shows that there was a clear difference of Ct values between the control group and streptavidin-coated tubes, which showed the intense adsorptive ability between biotinylated aptamer and streptavidin-coated tubes. In analysis of the Ct values at different concentrations of streptavidin, the Ct value reached the lowest level when the concentration of streptavidin was 2.5 ng mL -1 . This graph also shows that the Ct values decreased with the increase of the amount of aptamer when aptamer levels were below 10.0 nM andalso that the Ct values increased with the amount of aptamer above 10.0 nM mainly because of steric hindrance. Thus, 2.5 ng mL -1 of streptavidin and 10 nM of aptamer were the optimal conditions for RT-qPCR amplification. AFM 1 determinationUnder optimal conditions, the amplification curves and calibration curve of this aptasensor for different levels of AFM 1 with AFM 1 DNA1 were determined by RT-qPCR (Fig. 1). As is shown in Fig. 1(A), the cycle number increased with an increase in AFM 1 . More complementary ssDNA would be released when more AFM 1 is present in the reaction system, which leads to a decrease in the amount of the PCR template and an increase in cycle number. Meanwhile, a good linear relationshipbetween Ct values and AFM 1 levels in the range of 1×10 -4 to 1 µg L -1 was obtained as (Table 1). Specificity analysisThe specificity of the aptasensor plays an important role in the development and practicality of this method. In ...

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A label-free silver wire based impedimetric immunosensor for detection of aflatoxin M1 in milk

Cited by 83 publications

References 36 publications

Highly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier

Highly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

A qPCR aptasensor for sensitive detection of aflatoxin M1

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