A kinetic study on crystallization of calcium gluconate in external loop airlift column and stirred tank for an immobilized glucose oxidase reaction with crystallization

Bao, Jie; Koumatsu, Kenichi; Arimatsu, Yoshihiro; Furumoto, Keiji; Yoshimoto, Makoto; Fukunaga, Kimitoshi; Nakao, Katsumi

doi:10.1016/s1369-703x(02)00212-7

Cited by 19 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…simultaneous reactions for sorbitol and gluconic acid production in the bioreactor, the gluconate productivity is significantly greater than the gluconic acid productivity in soluble form without precipitation. The productivity of gluconic acid obtained by calcium gluconate precipitation method was 0.45 mole (50 g l −1 for CaCl 2 ) as compared to that of soluble form was only 0.29 mole under the reaction conditions, 40 • C and pH 5-6 (Bao et al, 2003). Further improvement for increased productivity is under investigation by manipulating the initial feed substrate concentrations, molar ratio of the two substrates, combined effects of cofactors and controlled in situ precipitation of calcium gluconate.…”

Section: Discussionmentioning

confidence: 89%

Sorbitol production using recombinant Zymomonas mobilis strain

Liu

Dong

Zhong

et al. 2010

Journal of Biotechnology

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 89%

Sorbitol production using recombinant Zymomonas mobilis strain

Liu

Dong

Zhong

et al. 2010

Journal of Biotechnology

Self Cite

View full text Add to dashboard Cite

“…GOD use is also an alternative way to production of H2O2 for textile bleaching (Tzanov et al 2002, Farooq et al 2013, Reis et al 2017, Qui et al 2017 or milk processing (Zhou &Lim 2009, Cruz et al 2013, Cruz et al 2012, Szweda et al 2013, Batista et al 2015. There are several studies about gluconic acid or gluconate salt production by GOD (Huang et al 2019, Han et al 2018, Ramezani et al 2013, Mariam et al 2010, Arimatsu et al 2004, Bao et al 2003, Shah & Kothari, 1993. GOD has analytical application to determine glucose levels of blood, serum, fruit juice etc., and it is also the most used enzyme in preparation of glucose biosensors (Ozyilmaz et al 2018, Khun et al 2012, Guo et al 2011, Salimi & Noorbahsh, 2011, Mazeiko et al 2013, Ozyilmaz et al 2007, Uang & Chou, 2003, Ekiz et al 2011, Wang et al 2013).…”

Section: Figure 1 Reaction Mechanism Of Godmentioning

confidence: 99%

Glucose Oxidase Applications and Comparison of the Activity Assays

Özyılmaz¹

2019

Natural and Engineering Sciences

View full text Add to dashboard Cite

Glucose Oxidase (GOD) oxidizes glucose to -gluconolactone in presence of moleculer oxygen by forming hydrogen peroxide. As a result of the catalysed reaction, GOD is widely used in cases where glucose or molecular oxygen should be removed to extend the shelf life of foods or used in the production of controlled hydrogen peroxide or gluconic acid. One of the most important application areas of GOD is the construction of the glucose biosensors. There are several studies about GOD purification, immobilization, industrial and analytical applications, so, fast and sensitive determination of GOD activity is essential for these studies. In this study, GOD activity determination methods were reviewed mainly four approaches: determination of decrease in glucose or oxygen concentration and determination of increase in hydrogen peroxide or gluconic acid levels.

show abstract

“…Their reaction system allowed productivities from 5.78 to 5.88 g L −1 h −1 to be achieved. The oxygen transfer rate is a key parameter in this kind of reaction, thus an efficient supplement and mass transfer of oxygen can be achieved using a suitable bioreactor, for example, airlift or bubble column bioreactors [4,7,[23][24][25][26][27][28]. Besides, the use of a co-immobilized multi-enzyme system composed of GOD and CAT could be kinetically advantageous, because CAT releases oxygen which allows a better saturation of the GOD by this substrate [20,22,29].…”

Section: Introductionmentioning

confidence: 99%

Combi-CLEAs of Glucose Oxidase and Catalase for Conversion of Glucose to Gluconic Acid Eliminating the Hydrogen Peroxide to Maintain Enzyme Activity in a Bubble Column Reactor

et al. 2019

View full text Add to dashboard Cite

In this study combined cross-linked aggregates of catalase from bovine liver and glucose-oxidase from Aspergillus niger were prepared, and the effects of the precipitant and crosslinking agents, as well as the use of bovine serum albumin (BSA) as a feeder protein, on enzyme immobilization yield and thermal stability of both enzymes, were evaluated. Combi- crosslinking of enzyme aggregates (CLEAs) prepared using dimethoxyethane as precipitant, 25 mM glutaraldehyde and BSA/enzymes mass ratio of 5.45 (w/w), exhibited the highest enzyme activities and stabilities at 40 °C, pH 6.0, and 250 rpm for 5 h. The stability of both immobilized enzymes was fairly similar, eliminating one of the problems of enzyme coimmobilization. Combi-CLEAs were used in gluconic acid (GA) production in a bubble column reactor operated at 40 °C, pH 6.0 and 10 vvm of aeration, using 26 g L−1 glucose as the substrate. Results showed conversion of around 96% and a reaction course very similar to the same process using free enzymes. The operational half-life was 34 h, determined from kinetic profiles and the first order inactivation model. Combi-CLEAs of glucose-oxidase and catalase were shown to be a robust biocatalyst for applications in the production of gluconic acid from glucose.

show abstract

A kinetic study on crystallization of calcium gluconate in external loop airlift column and stirred tank for an immobilized glucose oxidase reaction with crystallization

Cited by 19 publications

References 11 publications

Sorbitol production using recombinant Zymomonas mobilis strain

Sorbitol production using recombinant Zymomonas mobilis strain

Glucose Oxidase Applications and Comparison of the Activity Assays

Combi-CLEAs of Glucose Oxidase and Catalase for Conversion of Glucose to Gluconic Acid Eliminating the Hydrogen Peroxide to Maintain Enzyme Activity in a Bubble Column Reactor

Contact Info

Product

Resources

About