Sorbitol production using recombinant Zymomonas mobilis strain

Liu, Changjun; Dong, Hongwei; Zhong, Jian‐Jiang; Ryu, Dewey D. Y.; Bao, Jie

doi:10.1016/j.jbiotec.2010.04.008

Cited by 26 publications

(22 citation statements)

References 18 publications

(14 reference statements)

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“…). Attempts to divert carbon flux to these products has been reported using metabolic modelling and metabolic engineering (Lee et al ., ; Liu et al ., ; Kim et al ., ); these potential products were discussed in detail in other reviews (Rogers et al ., ; He et al ., ). For example, recombinant Z. mobilis strains transformed with Leuconosloc sp.…”

Section: Bioproductsmentioning

confidence: 97%

Zymomonas mobilis as a model system for production of biofuels and biochemicals

Yang

Fei

Zhang

et al. 2016

Microbial Biotechnology

166

110

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Summary Zymomonas mobilis is a natural ethanologen with many desirable industrial biocatalyst characteristics. In this review, we will discuss work to develop Z. mobilis as a model system for biofuel production from the perspectives of substrate utilization, development for industrial robustness, potential product spectrum, strain evaluation and fermentation strategies. This review also encompasses perspectives related to classical genetic tools and emerging technologies in this context.

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Section: Bioproductsmentioning

confidence: 97%

Zymomonas mobilis as a model system for production of biofuels and biochemicals

Yang

Fei

Zhang

et al. 2016

Microbial Biotechnology

166

110

View full text Add to dashboard Cite

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“…However, Z. mobilis presents a narrower range of useful substrates for fermentation than yeasts, because it only has the ability to hydrolyze glucose, fructose and sucrose (Swings and De Ley 1977). Additionally, using sucrose as substrate, Z. mobilis can form other products such as sorbitol, levan polysaccharides and fructose oligosaccharides (FOS), causing a significant decrease in ethanol production (Skotnicki et al 1981;Liu et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Bioethanol production in batch mode by a native strain of Zymomonas mobilis

Pinilla

Torres

Ortiz

2011

World J Microbiol Biotechnol

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Two wild strains of Zymomonas mobilis were isolated (named as ML1 and ML2) from sugar cane molasses obtained from different farms of Santander, Colombia. Initially, selection of the best ethanol-producer strains was carried out using ethanol production parameters obtained with a commercial strain Z. mobilis DSM 3580. Three isolated strains were cultivated in a culture medium containing yeast extract, peptone, glucose and salts, at pH 6 and 32°C with stirring rate of 65 rpm during 62 h. The best results of ethanol production were obtained with the native strain ML1, reaching a maximum ethanol concentration of 79.78 g l -1 . ML1 and ML2 strains were identified as Z. mobilis, according to the morphology, biochemical tests and molecular characterization by PCR of specific DNA sequences from Z. mobilis. Subsequently, the effect of different nitrogen sources on production of ethanol was evaluated. The best results were obtained using urea at a 0.73 g/l. In this case, maximum concentration of ethanol was 83.81 g l -1 , with kinetic parameters of yield of ethanol on biomass (Y P/X) = 69.01(g g -1 ), maximum volumetric productivity of ethanol (Qp max ) = 2.28 (g l -1 h -1 ), specific productivity of ethanol (q P ) = 3.54 (h -1 ) and specific growth rate (l) = 0.12 h -1 . Finally, we studied the effect of different culture conditions (pH, temperature, stirring, C/N ratio) with a Placket-Burman 0 s experimental design. This optimization indicated that the most significant variables were temperature and stirring. In the best culture conditions a significant increase in all variables of response was achieved, reaching a maximum ethanol concentration of 93.55 g l -1 .

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“…For further improvement of sorbitol production, overexpression of GFOR is an attractive strategy to improve its efficiency. As reported by Liu et al , an engineered strain harboring plasimd pHW20a- gfor , showed higher sorbitol yield than the wild strain [109]. On the other hand, although Z. mobilis could convert a mixture of glucose and fructose into sorbitol with high efficiency, the cost of the substrate may be very high.…”

Section: Reviewmentioning

confidence: 99%

“…Cazetta et al investigated sorbitol production from sugar cane molasses by Z. mobilis , which showed the best conditions for sorbitol production containing 300 g/L total reducing sugars (TRS) in the culture medium [108]. Actually, to improve the sorbitol yield, various cell permeabilization methods, that is toluene [104], dried Z. mobilis cells, CTAB [105], metal ions [109], which inhibited key enzymes of the ED pathway and led to decreased ethanol concentration.…”

Section: Reviewmentioning

confidence: 99%

Zymomonas mobilis: a novel platform for future biorefineries

Han

et al. 2014

Biotechnol Biofuels

184

137

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Biosynthesis of liquid fuels and biomass-based building block chemicals from microorganisms have been regarded as a competitive alternative route to traditional. Zymomonas mobilis possesses a number of desirable characteristics for its special Entner-Doudoroff pathway, which makes it an ideal platform for both metabolic engineering and commercial-scale production of desirable bio-products as the same as Escherichia coli and Saccharomyces cerevisiae based on consideration of future biomass biorefinery. Z. mobilis has been studied extensively on both fundamental and applied level, which will provide a basis for industrial biotechnology in the future. Furthermore, metabolic engineering of Z. mobilis for enhancing bio-ethanol production from biomass resources has been significantly promoted by different methods (i.e. mutagenesis, adaptive laboratory evolution, specific gene knock-out, and metabolic engineering). In addition, the feasibility of representative metabolites, i.e. sorbitol, bionic acid, levan, succinic acid, isobutanol, and isobutanol produced by Z. mobilis and the strategies for strain improvements are also discussed or highlighted in this paper. Moreover, this review will present some guidelines for future developments in the bio-based chemical production using Z. mobilis as a novel industrial platform for future biofineries.

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Sorbitol production using recombinant Zymomonas mobilis strain

Cited by 26 publications

References 18 publications

Zymomonas mobilis as a model system for production of biofuels and biochemicals

Zymomonas mobilis as a model system for production of biofuels and biochemicals

Bioethanol production in batch mode by a native strain of Zymomonas mobilis

Zymomonas mobilis: a novel platform for future biorefineries

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