<i>Zymomonas mobilis</i> as a model system for production of biofuels and biochemicals

Yang, Shihui; Fei, Qiang; Zhang, Yaoping; Contreras, Lydia M.; Utturkar, Sagar M.; Brown, Steven D.; Himmel, Michael E.; Zhang, Min

doi:10.1111/1751-7915.12408

Cited by 162 publications

(112 citation statements)

References 203 publications

(263 reference statements)

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“…Ethanol production from lignocellulosic biomass provides opportunities for exploring the advantage of Z. mibilis , because only glucose is released from cellulose hydrolysis, and in the meantime this species can be engineered with xylose isomerase to convert xylose to xylulose directly without cofactor imbalance and xylitol accumulation that are intrinsic to S. cerevisiae engineered with xylose reductase and xylitol dehydrogenase for ethanol production from xylose (Jeffries, ; M. Zhang, Eddy, Deanda, Finkelstein, & Picataggio, ). In addition, Z. mobilis is also a potential host to be engineered for biorefinery to produce biobased chemicals such as 2, 3‐butanediol (M. He et al, ; Yang, Fei et al, ; Yang, Mohagheghi et al ).…”

Section: Introductionmentioning

confidence: 99%

Contribution of cellulose synthesis, formation of fibrils and their entanglement to the self‐flocculation of Zymomonas mobilis

Xia

Liu

Zhao

et al. 2018

Biotech & Bioengineering

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Due to the unique Entner-Doudoroff pathway, Zymomonas mobilis has been acknowledged as a potential host to be engineered for biorefinery to produce biofuels and biobased chemicals. The self-flocculation of Z. mobilis can make the bacterial cells self-immobilized within bioreactors for high density to improve product productivities, and in the meantime enhance their tolerance to stresses, particularly product inhibition and the toxicity of byproducts released during the pretreatment of lignocellulosic biomass. In this work, we explored mechanism underlying such a phenotype with the self-flocculating strain ZM401 developed from the regular non-flocculating strain ZM4. Cellulase de-flocculation and the restoration of the self-flocculating phenotype for the de-flocculated bacterial cells subjected to culture confirmed the essential role of cellulose biosynthesis in the self-flocculation of ZM401. Furthermore, the deactivation of both Type I and Type IV restriction-modification systems was performed for ZM4 and ZM401 to improve their transformation efficiencies. Comparative genome analysis detected the deletion of a thymine from ZMO1082 in ZM401, leading to a frame-shift mutation for the putative gene to be integrated into the neighboring downstream gene ZMO1083 encoding the catalytic subunit A of cellulose synthase, and consequently created a new gene to encode a larger transmembrane protein BcsA_401 for more efficient synthesis of cellulose as well as the development of cellulose fibrils and their entanglement for the self-flocculation of the mutant. These speculations were confirmed by the morphological observation of the bacterial cells under scanning electron microscopy, the impact of the gene deletion on the self-flocculation of ZM401, and the restoration of the self-flocculating phenotype of ZM401 ΔbcsA by the gene complementation. The progress will lay a foundation not only for fundamental research in deciphering molecular mechanisms underlying the self-flocculation of Z. mobilis and stress tolerance associated with the morphological change but also for technological innovations in engineering non-flocculating Z. mobilis and other bacterial species with the self-flocculating phenotype.

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Section: Introductionmentioning

confidence: 99%

Contribution of cellulose synthesis, formation of fibrils and their entanglement to the self‐flocculation of Zymomonas mobilis

Xia

Liu

Zhao

et al. 2018

Biotech & Bioengineering

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“…Furthermore, the availability of multiple genome sequences for 12 Zymomonas strains with small genome size around 2 Mb (Seo et al 2005;Yang et al 2009a;Zhao et al 2012), multiple genome-scale metabolic models (Kalnenieks et al 2014;Pentjuss et al 2013;Widiastuti et al 2011), and versatile genetic engineering strategies (Jia et al 2013;Shui et al 2015;Tan et al 2016) also accelerates the research progress in Z. mobilis. Z. mobilis has also been engineered for the production of sorbitol, gluconic acid, levan, 2,3-butanediol, isobutanol, and other biochemicals, which is proposed as an ideal microbial chassis for future synthetic biology and biorefinery (He et al 2014;Yang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.

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“…Exceptions of alcohol producing bacteria such as Zymomonas mobilis, Clostridium acetobutylicum, and Klebsiella pneumonia exist. Z. mobilis is a natural ethanologen which possess desirable characteristics such as high specific productivity, high alcohol tolerance, a broad pH range for production (pH 3.5 to 7.5) and it is generally regarded as safe (GRAS) [84]. The bacterium is mostly utilized for biofuel production [85] although it has also been isolated from some alcoholic beverages such as pulque [11,54] and palm wine [86] where it is responsible for the production of ethanol, CO 2 , acetaldehyde and H 2 S [87].…”

Section: Bacteriamentioning

confidence: 99%

“…The banana beverage is common in Rwanda (urwagwa) [42], DR Congo (kasiksi), Burundi (isongo) [116], Uganda (tonto) [127], Tanzania (mbege) [128] and Kenya (urwaga) [127] where it is known by other local names. There have been reports of urwagwa having an alcoholic content ranging from 7% to 18.1% (v/v) [34,84]. The process of fermentation [42] involves the extraction of the banana juice from ripe bananas (which contain glucose, sucrose and fructose) by addition of water to the banana juice in the ratio of 3:1.…”

Section: Urwagwamentioning

confidence: 99%

Microbial and Chemical Diversity of Traditional Non-Cereal Based Alcoholic Beverages of Sub-Saharan Africa

2018

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Abstract:Fermentation remains an important food preparation technique of health, cultural and economic importance throughout the world. In Sub-Saharan Africa, traditional alcoholic fermentation of cereal and non-cereal based substrates into alcoholic beverages is deeply rooted in the society. Although a multitude of traditional alcoholic beverages from cereal substrates are well researched and documented, their non-cereal based counterparts, mostly produced from indigenous, inexpensive substrates, remain less well studied. In addition, reports of health problems associated with non-cereal based alcoholic beverages produced from spontaneous fermentation are a major cause of concern. This review aims to highlight the microbiological and chemical profiles of these non-cereal based alcoholic beverages with a focus on the Sub-Saharan region. Here, we underscore the importance of the microbial repertoire and the substrates thereof in attaining aromatic complexity and a characteristic taste in these beverages. These aspects are an important starting point towards the potential commercialization of these complex aromatic non-cereal based traditional beverages.

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Zymomonas mobilis as a model system for production of biofuels and biochemicals

Cited by 162 publications

References 203 publications

Contribution of cellulose synthesis, formation of fibrils and their entanglement to the self‐flocculation of Zymomonas mobilis

Contribution of cellulose synthesis, formation of fibrils and their entanglement to the self‐flocculation of Zymomonas mobilis

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Microbial and Chemical Diversity of Traditional Non-Cereal Based Alcoholic Beverages of Sub-Saharan Africa

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