A Kinetic Study of Acetylcholinesterase Inhibition by Fractions of Oleo diox Roxb.leaf and Curculigo orchioides Gaertn Rhizome for the Treatment of Alzheimer’s Disease

Abstract

Introduction and Aim: Acetylcholinesterase (AChE) has an important role in the biochemical studies indicating that AChE accelerates the formation of amyloid-β fibril and forms AChE-Aβ complexes in the brain. The medicinal plants of Curculigoorchioides Gaertn., and Oleo diox Roxb. collected from Western Ghat region of Kodagu, Karnataka, India have many medicinal values such as antioxidant, anti-cancer, anti-diabetic, neuroprotective activity and anti-inflammatory property. Aim of the study was to check whether C. orchioides Gaertn. and Oleo diox Roxb. fractions significantly inhibit acetylcholinesterase in different concentration-dependent manner(1-3 mg/mL).
 Results: Plant fraction concentrations resulted in >60%AChE inhibition as compared with the standard Galantamine. The IC50 value was 0.15 mg/mL which was calculated from the equation of the percentage inhibition curve for the test or plant fraction. The Lineweaver-Burk plot indicated that the fraction followed competitive and non-competitive inhibition kinetics. Finally, we conclude that the type of plot exhibited by the C. orchioides Gaertn. and Oleo dioxRoxb. fractions has a possible therapeutic application for improving memory and other cognitive functions.The medicinal plant-based drugs are known to have limitations due to their fewer side effects and problems associated with bioavailability, which necessitates the interest in finding better AChE inhibitors from plant sources.

In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

“…The extracts demonstrated strong AChE inhibition, with values > 60% at various sample concentrations (10, 20, 40, 60, 80, and 100 μg/mL). The phenolic compounds are known to be neuroprotective exhibiting AChE inhibitory activity and could be one of the main approaches for the treatment of AD ( Pratap and Shantaram, 2020b ) ( Pratap et al, 2021 ). The neuroprotective action of the methanolic extract of Olea dioica Roxb.…”

Phytochemistry and pharmacology of Curculigo orchioides Gaertn: A review

“…Acetylcholinesterase was effectively inhibited by the extracts of C. orchioides rhizomes, suggesting their potential use in the treatment of Alzheimer's disease (Pratap and Shantaram, 2019). Curculigoside remarkably reduced NMDA-induced loss of neuron cells, apoptosis, necrosis, excitotoxicity, and reactive oxygen species (ROS) production in cultured cortical neurons.…”