A kinetic method for the determination of haptoglobin as haemoglobin binding capacity

Standing, Susan; Price, C P

doi:10.1016/0009-8981(76)90239-4

Cited by 16 publications

(5 citation statements)

References 13 publications

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“…This binding enhances the pseudoperoxidase activity of hemoglobin (Hb) and this property was used in the first methods described for the quantitation of lip (1). Many modifications of this enzymatic method, some permitting the use of automatic analyzers, have since been published (2)(3)(4). Other methods are based upon the altered spectrophotometric properties of bound hemoglobin (5,6) or upon the separation of the Hb-Hp complex from unbound hemoglobin (2,7,8).…”

mentioning

confidence: 99%

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Rijn¹,

Wilt²,

Stroes³

et al. 1987

Clinical Biochemistry

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Comparison of the turNdirnetric immunossay of haptoglobin with a reference method (the RID technique with appropriate correction for phenetype) clearly showed the turbidimetric assay to be phenotypedependent. Correction f~ctors for the three main phenotypes were calculated and reference values determined.The observed overall reference range of haptoglobin, irrespective of the phenotype, was 0.56-3.76 g/L. After correction for phenotype, reference values for the three types were: Hp 1-1 : 0.77-2.49; Hp 2-1:0.86-4.76; and Hp 2-2:0.48-3.15 g Hp/L, From our reference values for the several phenotypes of haptoglobin it may be argued that phenotyping is desirable only when the uncorrected haptoglobin value, determined by turbidimetry, is within the range 0.38-0.98 g/L. Limited to hemolytic diseases, phenotype determination can be omitted beyond this range.

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mentioning

confidence: 99%

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Rijn¹,

Wilt²,

Stroes³

et al. 1987

Clinical Biochemistry

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“…Furthermore, these variations were similar or better than the reported values for previous HbBC quantification methods. The difference in peroxidase activity had larger variations (7.6% using different standards and 2.6% using the same standard curve 54 ). Spectrophotometric differences between free Hb and Hb-Hp had more than 10% error.…”

Section: Total Protein Assaymentioning

confidence: 95%

Tangential flow filtration of haptoglobin

Pires

Palmer

2020

Biotechnology Progress

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Haptoglobin (Hp) is a plasma glycoprotein that scavenges cell-free hemoglobin (Hb). Hp has various potential therapeutic applications, but it has been mainly studied for treatment of acute hemolytic conditions that can arise from situations such as massive blood transfusion, infusion of stored red blood cells, severe burns, trauma, sepsis, radiation injury, and others. Therefore, Hp may also be beneficial during chronic hemolytic disease states such as hereditary spherocytosis, nocturnal hemoglobinuria, sickle-cell anemia, and malaria. Various methods have been developed to purify Hp from plasma or plasma fractions. However, none of these methods have exploited the large molecular weight (MW) range distribution of Hp polymers to easily isolate Hp from other plasma proteins. The present study used tangential flow filtration (TFF) to isolate polymeric Hp from plasma proteins using human Fraction IV (FIV) as the starting material. After removal of insoluble material from a suspension of FIV paste, the protein mixture was clarified on a 0.2 μm hollow fiber (HF) TFF filter. The clarified protein solution was then bracketed based on protein MW using HF filters with MW cutoffs (MWCOs) of 750, 500, and 100 kDa. Using untreated FIV, the Hp purity of the main bracket was~75% with a total Hb binding capacity (HbBC) yield of 1.2 g starting from 500 g of FIV paste. However, pretreatment of FIV with fumed silica to remove lipoproteins increased Hp purity to >95% with a HbBC yield of 1.7 g per 500 g of FIV. Taken together this study provides a novel and scalable method to purify Hp from plasma or plasma fractions.

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“…Erythrocyte osmotic fragility was determined before and after incubation for 24 h (7). Haptoglobin was determined as haemoglobin-binding capacity (24).…”

Section: Methodsmentioning

confidence: 99%

Changes in Peripheral Blood Cells and Serum Ferritin in Lysinuric Protein Intolerance

et al. 1980

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Twenty-seven patients with lysinuric protein intolerance, an autosomal recessive disorder of diamino acid transport, have been followed at our hospital since 1965. Many of the patients had recurrent mild normochromic anaemia without apparent iron deficiency, and leuko- and/or thrombocytopenia. Serum ferritin concentrations were inappropriately high. Serum ferritin concentration appears to be a valuable index of the control of this disease.

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A kinetic method for the determination of haptoglobin as haemoglobin binding capacity

Cited by 16 publications

References 13 publications

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Tangential flow filtration of haptoglobin

Changes in Peripheral Blood Cells and Serum Ferritin in Lysinuric Protein Intolerance

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