APrunus mumeExtract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

Abstract

Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.

Hypouricemic effect of the methanol extract fromPrunus mumefruit in mice

“…Consistent with our study, an early study performing with the same protocol showed that potassium oxonate induced the abnormality of uric acid in serum, liver and urine, and allopurinol reversed the changes (Wang et al, 2010). Our present findings showed that MPMF, in addition to having many previously described biological activities (Chuda et al, 1999;Kim et al, 2008;Kono et al, 2011;Tsuji et al, 2011;Seneviratne et al, 2011), may be a potent uric acid-lowering agent.…”

“…The various parts of the P. mume tree (such as fruit, seed, and leaf) have been used as a health food or medicinal material in traditional herb medicine. It has recently been reported that P. mume fruit improved blood fluidity (Chuda et al, 1999), exerted ergogenic and physical fatigue recovery activities (Kim et al, 2008), stimulated the proliferation and osteoblastic differentiation of cells (Kono et al, 2011), enhanced the immune function by stimulating innate immune cells (Tsuji et al, 2011), and exhibited antimicrobial activity against pathogenic oral bacteria (Seneviratne et al, 2011). Moreover, it is particularly used in traditional Chinese medical preparations as a remedy for gout (Zhao & Yang, 2002;Chen et al, 2007).…”

Diosgenin prevents bone loss on retinoic acid-induced osteoporosis in rats

“…Citrus fruits are rich in micronutrients, limonoids, and polyphenolic compounds, which were important in improving the BMD and bone biomechanical properties and decreasing the bone resorption [38]. A recent report showed that an extract of Prunus mume affected the proliferation and differentiation of preosteoblastic MC3T3-E1 cells [39]. Almost all these researches based on the mechanisms that antioxidant vitamins may exert favorable effects on BMD and osteoporotic risk by scavenging free radicals and thereby reducing oxidative stress [40].…”

3,4-Dihydroxybenzaldehyde Derived from <i>Prunus mume</i> Seed Inhibits Oxidative Stress and Enhances Estradiol Secretion in Human Ovarian Granulosa Tumor Cells

“…For example, an in vitro study showed that the concentrated fruit juice of P. mume may prevent cardiovascular diseases because of its anti-oxidative effect on angiotensin II-induced ROS generation [30]. In addition, P. mume extract may attenuate osteoporosis because it stimulates the differentiation of osteoblastic cells [16]. An epidemiological study in humans showed that P. mume had an inhibitory effect on Helicobacter pylori -related chronic gastritis [9].…”