A hybrid small‐diameter tube fabricated from decellularized aortic intima‐media and electrospun fiber for artificial small‐diameter blood vessel

Abstract

Hybrid small‐diameter tubes were fabricated by wrapping decellularized aortic intima‐media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima‐media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima‐media with electrospun fibers generates a small‐diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1064–1070, 2019.

Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo

“…There was no obvious inflammatory response at the implantation site of decellularized PDL and mandible bone, indicating that there was no immune rejection. We previously reported that strong acute inflammation did not occur when HHP-decellularized porcine tissue was implanted into rabbits, rats, and mice [10,11,12,13,14,15,16,17]. Thus, HHP-decellularized mouse mandible bone with PDL is tolerated by rat host cells.…”

“…Many techniques for decellularization have been developed and the appropriate method is selected based on the tissue of interest [4,5,6,7,8,9]. We previously reported that high hydrostatic pressure (HHP) decellularization, in which cells are ruptured by HHP and cell components are washed out, can effectively decellularize tissues such as blood vessel [10,11,12,13], cornea [14,15], and bone and bone marrow [16,17] while preserving the tissue structure. Additionally, HHP-decellularized tissues showed good biocompatibility and regenerative capacity.…”

Extracellular Matrix for Small-Diameter Vascular Grafts

“…However, human arteries are stiffer than porcine aortas and other porcine arteries. [104][105][106] Wu et al circumvented the mechanical mismatch across the two species by covering the porcine aorta graft with electrospun polyurethane. 106 The polyurethane increased stiffness of the graft and supported the in vitro 2D cell culture of HUVECs and normal hDFs (NHDFs) for 2 weeks.…”

“…[104][105][106] Wu et al circumvented the mechanical mismatch across the two species by covering the porcine aorta graft with electrospun polyurethane. 106 The polyurethane increased stiffness of the graft and supported the in vitro 2D cell culture of HUVECs and normal hDFs (NHDFs) for 2 weeks. 106 In a study by Negishi et al, sheets of porcine aorta were decellularized by using high hydrostatic pressure since traditional detergent-based methods of decellularization, including SDS and Triton X-100, did not work.…”

Effect of introduction of tetrabutylammonium bromide on properties of poly (L‐lactic acid) tubular scaffold prepared by electrospinning

“…At present, large‐ and medium‐caliber blood vessels have been studied and matured, whereas small‐caliber blood vessels (diameter <6 mm) are prone to thrombosis during use. To solve the problem of embolisation of small blood vessels, much research effort has been devoted to the improvement of the preparation process and the selection of polymers [2, 4]. In this context, electrospinning technology has become a research hotspot for the development of biomaterials, since it can produce nano‐sized porous structures that can effectively simulate the composition and structure of the extracellular matrix.…”