Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo

Nakamura, Naoko; Ito, Ai; Kimura, Tsuyoshi; Kishida, Akio

doi:10.3390/ijms20133277

Cited by 20 publications

(26 citation statements)

References 23 publications

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“…From a medical point of view, it is recommended to use lower values because higher values could trigger root resorption, particularly in cases with abnormal root shape and increased root length [16,17]. Taking into consideration that the PDL has an absorption function and facilitates the displacement and alignment of teeth during orthodontic treatment [18] in the areas with decreased thickness, the displacement is accelerated or significantly increased. The correlation between the PDL's thickness and displacement is displayed throughout our study.…”

Section: Discussionmentioning

confidence: 99%

Finite Element Analysis of Mandibular Anterior Teeth with Healthy, but Reduced Periodontium

et al. 2021

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Finite element analysis studies have been of interest in the field of orthodontics and this is due to the ability to study the stress in the bone, periodontal ligament (PDL), teeth and the displacement in the bone by using this method. Our study aimed to present a method that determines the effect of applying orthodontic forces in bodily direction on a healthy and reduced periodontium and to demonstrate the utility of finite element analysis. Using the cone-beam computed tomography (CBCT) of a patient with a healthy and reduced periodontium, we modeled the geometric construction of the contour of the elements necessary for the study. Afterwards, we applied a force of 1 N and a force of 0.8 N in order to achieve bodily movement and to analyze the stress in the bone, in the periodontal ligament and the absolute displacement. The analysis of the applied forces showed that a minimal ligament thickness is correlated with the highest value of the maximum stress in the PDL and a decreased displacement. This confirms the results obtained in previous clinical practice, confirming the validity of the simulation. During orthodontic tooth movement, the morphology of the teeth and of the periodontium should be taken into account. The effect of orthodontic forces on a particular anatomy could be studied using FEA, a method that provides real data. This is necessary for proper treatment planning and its particularization depends on the patient’s particular situation.

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Section: Discussionmentioning

confidence: 99%

Finite Element Analysis of Mandibular Anterior Teeth with Healthy, but Reduced Periodontium

et al. 2021

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“…In one study, HHP-decellularized liver powder was implanted in a rat model of acute myocardial infarction, inducing cell migration and neovascularization, thus resulting in the suppression of myocardial necrosis [ 16 ]. The role of ECM microstructure in the good in vivo performance of HHP-decellularized tissues has been previously investigated [ 17 , 18 , 19 , 20 , 21 ]; however, the mechanism involving the bioactive components is still unknown. In this study, to evaluate whether MBVs are present in decellularized tissues, regardless of decellularization method, MBVs were extracted, and their presence was identified within HHP-decellularized tissues.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues

Kobayashi

Ishida

Hashimoto

et al. 2022

IJMS

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Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.

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“…Compared to chemically derived d-ECMs, the HHP d-ECM is nontoxic, because no surfactants are used in the decellularization process. We have also identified properties of HHP d-ECM, including tissue structure [ 10 ], permeability [ 11 ], mechanical characteristics [ 12 ], and biocompatibility [ 13 ], that are preferable to those of d-ECMs prepared with surfactant methods, such as sodium dodeoxy sulfate (SDS) and sodium deoxycholate (SDC). Furthermore, HHP d-ECM generated using aortic and corneal tissue performed well during in vivo implantation, and resulted in the re-cellularization of recipient cells [ 4 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Elastic Modulus of ECM Hydrogels Derived from Decellularized Tissue Affects Capillary Network Formation in Endothelial Cells

Kobayashi

Kadota

Hashimoto

et al. 2020

IJMS

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Recent applications of decellularized tissue have included the use of hydrogels for injectable materials and three-dimensional (3D) bioprinting bioink for tissue regeneration. Microvascular formation is required for the delivery of oxygen and nutrients to support cell growth and regeneration in tissues and organs. The aim of the present study was to evaluate the formation of capillary networks in decellularized extracellular matrix (d-ECM) hydrogels. The d-ECM hydrogels were obtained from the small intestine submucosa (SIS) and the urinary bladder matrix (UBM) after decellularizing with sodium deoxycholate (SDC) and high hydrostatic pressure (HHP). The SDC d-ECM hydrogel gradually gelated, while the HHP d-ECM hydrogel immediately gelated. All d-ECM hydrogels had low matrix stiffness compared to that of the collagen hydrogel, according to a compression test. D-ECM hydrogels with various elastic moduli were obtained, irrespective of the decellularization method or tissue source. Microvascular-derived endothelial cells were seeded on d-ECM hydrogels. Few cells attached to the SDC d-ECM hydrogel with no network formation, while on the HHP d-ECM hydrogel, a capillary network structure formed between elongated cells. Long, branched networks formed on d-ECM hydrogels with lower matrix stiffness. This suggests that the capillary network structure that forms on d-ECM hydrogels is closely related to the matrix stiffness of the hydrogel.

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Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo

Cited by 20 publications

References 23 publications

Finite Element Analysis of Mandibular Anterior Teeth with Healthy, but Reduced Periodontium

Finite Element Analysis of Mandibular Anterior Teeth with Healthy, but Reduced Periodontium

Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues

Elastic Modulus of ECM Hydrogels Derived from Decellularized Tissue Affects Capillary Network Formation in Endothelial Cells

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