A holidic medium for Drosophila melanogaster

Piper, Matthew D. W.; Blanc, Eric; Leitão-Gonçalves, Ricardo; Yang, Mingyao; He, Xiaoli; Linford, Nancy J.; Hoddinott, Matthew P.; Hopfen, Corinna; Soultoukis, George A; Niemeyer, Christine; Kerr, Fiona; Pletcher, Scott D.; Ribeiro, Carlos; Partridge, Linda

doi:10.1038/nmeth.2731

Cited by 315 publications

(466 citation statements)

References 35 publications

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“…In contrast, flies reared on high protein diet (P:C 8:1) laid numerous eggs and visited high protein diets only later, towards the end of the experiment. These results thus confirm that when given a choice between complementary foods, D. melanogaster mated females exhibit compensatory feeding, which enables them to balance their intake of protein and carbohydrates to reach nutritional states maximising egg production (Lee et al, 2008(Lee et al, , 2013Piper et al, 2014;Ribeiro and Dickson, 2010). Our analyses of the performances of larvae confined to specific diets show that development was impaired on high carbohydrate diets (P:C 1:16), as illustrated by the 15% decrease in survival, 30% increase in egg-to-adult development duration and 30% reduced learning scores in comparison to flies reared on more balanced diets.…”

Section: Discussionsupporting

confidence: 69%

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Drosophila females trade off good nutrition with high quality oviposition sites when choosing foods

Lihoreau

Poissonnier

Isabel

et al. 2016

Journal of Experimental Biology

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Animals, from insects to humans, select foods to regulate their acquisition of key nutrients in amounts and balances that maximise fitness. In species in which the nutrition of juveniles depends on parents, adults must make challenging foraging decisions that simultaneously address their own nutrient needs as well as those of their progeny. Here, we examined how the fruit fly Drosophila melanogaster, a species in which individuals eat and lay eggs in decaying fruits, integrate feeding decisions (individual nutrition) and oviposition decisions (offspring nutrition) when foraging. Using cafeteria assays with artificial diets varying in concentrations and ratios of protein to carbohydrates, we show that D. melanogaster females exhibit complex foraging patterns, alternating between laying eggs on high carbohydrate foods and feeding on foods with different nutrient contents depending on their own nutritional state. Although larvae showed faster development on high protein foods, both survival and learning performance were higher on balanced foods. We suggest that the apparent mismatch between the oviposition preference of females for high carbohydrate foods and the high performances of larvae on balanced foods reflects a natural situation where high carbohydrate ripened fruits gradually enrich in proteinaceous yeast as they start rotting, thereby yielding optimal nutrition for the developing larvae. Our findings that animals with rudimentary parental care uncouple feeding and egg-laying decisions in order to balance their own diet and provide a nutritionally optimal environment to their progeny reveal unsuspected levels of complexity in the nutritional ecology of parent-offspring interactions.

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Section: Discussionsupporting

confidence: 69%

“…Drosophila melanogaster (Lee, 2015;Lee et al, 2008Lee et al, , 2013Piper et al, 2014;Reddiex et al, 2013;Ribeiro and Dickson, 2010;Rodrigues et al, 2015); other fruit flies (Fanson et al, 2009;Matavelli et al, 2015)]. These effects of nutrition on physiology and behaviour greatly vary with age, sex and the mating status of individuals.…”

Section: Introductionmentioning

confidence: 99%

Drosophila females trade off good nutrition with high quality oviposition sites when choosing foods

Lihoreau

Poissonnier

Isabel

et al. 2016

Journal of Experimental Biology

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“…We used a modified liquid version of a synthetic diet (Piper et al., 2014), prepared entirely from synthetic components (recipes are in Tables A1–A3 in Appendix S1). Previous studies have used diets based on natural components, typically live or killed yeast as the protein source and sugar as the carbon source, which do not allow precise manipulation of nutrients and are confounded by responses to other dietary components (Piper & Partridge, 2007).…”

Section: Methodsmentioning

confidence: 99%

“…While virgin females mostly fed on carbohydrate‐based diets, mated females not only elevated their overall amount of food consumed (food quantity), but increased the concentration of protein ingested (food quality; Corrales‐Carvajal et al., 2016). In Drosophila females, egg production (reproductive output) has been directly linked to the nutritional state of female flies, with flies having a greater reproductive output on diets containing higher protein concentrations (Piper et al., 2014; Simmons & Bradley, 1997; Terashima & Bownes, 2004). These results allude to both a metabolic and behavioral shift for diet preference in response to a change in reproductive requirements (Kubli, 2010).…”

Section: Introductionmentioning

confidence: 99%

Dietary choices are influenced by genotype, mating status, and sex in Drosophila melanogaster

Camus

Huang

Reuter

et al. 2018

Ecology and Evolution

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Mating causes many changes in physiology, behavior, and gene expression in a wide range of organisms. These changes are predicted to be sex specific, influenced by the divergent reproductive roles of the sexes. In female insects, mating is associated with an increase in egg production which requires high levels of nutritional input with direct consequences for the physiological needs of individual females. Consequently, females alter their nutritional acquisition in line with the physiological demands imposed by mating. Although much is known about the female mating‐induced nutritional response, far less is known about changes in males. In addition, it is unknown whether variation between genotypes translates into variation in dietary behavioral responses. Here we examine mating‐induced shifts in male and female dietary preferences across genotypes of Drosophila melanogaster. We find sex‐ and genotype‐specific effects on both the quantity and quality of the chosen diet. These results contribute to our understanding of sex‐specific metabolism and reveal genotypic variation that influences responses to physiological demands.

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“…We also provide a short overview of the molecular mechanisms by which AA might control these two traits and their relationship. We focus on recent research in the Drosophila model, given that this system combines unrivaled genetic tools, a solid understanding of the effects of nutritional interventions, and the availability of holidic diets that now allow researchers to precisely control individual dietary components [14,[17][18][19]. …”

mentioning

confidence: 99%

Amino acid modulation of lifespan and reproduction in Drosophila

Hoedjes

Rodrigues

Flatt

2017

Current Opinion in Insect Science

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1Manipulating amino acid (AA) intake in Drosophila can profoundly affect lifespan and reproduction. Remarkably, AA manipulation can uncouple the commonly observed trade-off between these traits. This finding seems to challenge the idea that this trade-off is due to competitive resource allocation, but here we argue that this view might be too simplistic. We also discuss the mechanisms of the AA response, mediated by the IIS/TOR and GCN2 pathways. Elucidating how these pathways respond to specific AA will likely yield important insights into how AA modulate the reproduction-lifespan relationship. The Drosophila model offers powerful genetic tools, combined with options for precise diet manipulation, to address these fundamental questions. Introduction: dietary effects on lifespan and reproductionNutrition plays a primary role in shaping the physiology, life history and behavior of organisms, and nutritional interventions can have substantial health benefits [1]. Dietary restriction (DR), that is, the reduced intake of nutrients without malnutrition, has been the most widely studied nutritional intervention since the 1930s when it was first demonstrated that DR extends lifespan in rats. Since then, a large body of research has established positive effects of DR on longevity and age-related pathology in numerous organisms, ranging from yeast and worms to insects and mammals. At the same time, DR typically reduces reproductive output [2,3]. The fact that reduced food intake extends lifespan at the expense of reproduction makes the study of DR, and of dietary effects more generally, of key significance for our understanding of the commonly observed trade-off between reproduction and longevity [4,5].Originally, reduced intake of calories was thought to be responsible for the lifespan-extending effects of DR, but this view began to shift when studies in Drosophila showed that lifespan extension under DR does not depend on caloric restriction [5,6]. By testing diets with different nutrient compositions ('nutritional geometry framework') [7], it was found that the ratio of proteins to carbohydrates (P:C ratio), not overall energetic content, affects lifespan and reproduction in Drosophila [8,9]. Today, there is growing evidence that especially dietary proteins play a major role in mediating the effects of DR [10,11] (but see [12]). Remarkably, beyond the effects of the proteins themselves, recent work suggests that the building blocks of proteins, that is, specific amino acids (AA), can profoundly impact lifespan and associated traits. For example, in both flies and mice, restriction of dietary methionine can extend lifespan to the same extent as DR [13][14][15][16].Here, we give a brief review of how AA modulate lifespan and reproduction, and the trade-off between these traits. We also provide a short overview of the molecular mechanisms by which AA might control these two traits and their relationship. We focus on recent research in the Drosophila model, given that this system combines unrivaled genetic tools, a solid...

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A holidic medium for Drosophila melanogaster

Cited by 315 publications

References 35 publications

Drosophila females trade off good nutrition with high quality oviposition sites when choosing foods

Drosophila females trade off good nutrition with high quality oviposition sites when choosing foods

Dietary choices are influenced by genotype, mating status, and sex in Drosophila melanogaster

Amino acid modulation of lifespan and reproduction in Drosophila

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