A highly sensitive system for urea detection by using CdSe/ZnS core-shell quantum dots

Huang, Chin Ping; Li, Yaw-Kuen; Chen, Teng‐Ming

doi:10.1016/j.bios.2006.09.003

Cited by 142 publications

(39 citation statements)

References 17 publications

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“…[24][25][26][27] Recently, a few applications of QDs for the determination of urea appeared in the literature. 6,17,24 All of these studies are based on the pH change upon the reaction of urea and urease. Although most previous studies have been focused on QDs as a fluorescence sensor, their long lifetime allows the use of the phosphorescence mode, which has more advantages than fluorescence; for example, the spectral interferences from biologic matrices can be easily prevented in the phosphorescence mode.…”

Section: Introductionmentioning

confidence: 99%

“…Thus the selective determination of an analyte can be achieved via interaction with functionalized QD or non-functionalized QD. [22][23][24][25] QDs have been widely used as biosensors in biotechnology. [24][25][26][27] Recently, a few applications of QDs for the determination of urea appeared in the literature.…”

Section: Introductionmentioning

confidence: 99%

“…[22][23][24][25] QDs have been widely used as biosensors in biotechnology. [24][25][26][27] Recently, a few applications of QDs for the determination of urea appeared in the literature. 6,17,24 All of these studies are based on the pH change upon the reaction of urea and urease.…”

Section: Introductionmentioning

confidence: 99%

“…Ureasebased biosensors are alternative methods for the determination of urea levels. 6 To this end, a number of methods have been developed and reported such as potentiometry [7][8][9] , voltammetry 10,11 , conductometry [12][13][14] , ion selective electrode 15 , and spectrometry. 16,17 Quantum dots (QDs) are superior to organic dyes with their size-tunable photonic properties, quantum yield, and stability against photobleaching.…”

Section: Introductionmentioning

confidence: 99%

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Use of Water Soluble and Phosphorescent MPA Capped CdTe Quantum Dots for Detection of Urea

Oymak

Ertaş

Tamer

2018

tjps

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ÖZAmaç: Bu çalışmada 3-MPA kaplı CdTe kullanılarak üre tayini için yöntem geliştirilmiştir. Gereç ve Yöntemler: Yöntem üreaz enzimi ile ürenin reaksiyonu sonucu üretilen amonyağın ortam pH'sini değiştirmesi esasına dayanmaktadır. MPA kaplı CdTe'nin fosforesans sinyali pH 2.5-5.0 arasında üre konsantrasyonuyla doğrusal olarak artmaktadır. Optimum koşullar altında doğrusal aralık 0.016-0.16 mM (1-10 mg/L) gözlenebilme sınırı 3 s/b formülüne göre 0.003 mM (0.17 mg/L) olarak hesaplanmıştır. Bağıl standart sapma, 4 mg/L üre konsantrasyonunda %3.4 olarak hesaplanmıştır (n=7). Bulgular: Yöntem serum numunesine başarılı bir şekilde uygulanmıştır. Aynı serum numuneleri bağımsız bir laboratuvar tarafından analiz edilmiş ve sonuçlarda %95 güven aralığında istatistiki fark görülmemiştir (F testi). Sonuç: Önerilen yöntem serumda üre tayini için, örnek ön hazırlığına ihtiyaç duymayan, basit, seçici ve düşük maliyetlidir. Anahtar kelimeler: Fosforesan kuantum noktacık, MPA kaplı CdTe, üre Objectives: To describe a method for the determination of urea in blood serum using urease enzyme and 3-MPA-capped CdTe quantum dots. Materials and Methods:The method is based on the increase in pH of the solution as a result of the reaction between urea and urease, which causes an increase in the phosphorescence signal of MPA-CdTe quantum dots in the pH range of 2.5-5.0. Under the optimum conditions, the linear range of urea was 0.016-0.16 mM (1-10 mg/L) and the limit of detection based on 3 s/b was calculated as 0.003 mM (0.17 mg/L). The relative standard deviation was calculated as 3.4% at 4 mg/L urea concentration (n=7). Results:The method was applied to human serum samples. The same samples were analyzed by an independent laboratory and the results were not statistically different, at 95% confidence level (F test). Conclusion:The proposed method does not need sample pretreatment, is simple, selective, and cost-effective for the determination of urea in serum samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Use of Water Soluble and Phosphorescent MPA Capped CdTe Quantum Dots for Detection of Urea

Oymak

Ertaş

Tamer

2018

tjps

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“…Additionally, the development of biosensor utilizing bioconjugated system of QDs-enzyme serves for dual purpose; one is the immobilization of enzyme and second is application in biosensing based on changes in fluorescence intensity [15]. Fluorescence emission wavelength of the QDs is tunable via adjusting QDs size and customizing the nature of capping molecules, which are liable for surface charges modification for bimolecular coupling [16].…”

Section: Introductionmentioning

confidence: 99%

Cdse/Zns Capped Thiolate for Application in Glucose Sensing

Rahman¹,

Ariffin²,

Yusof³

et al. 2017

Biosens J

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A semiconducting water soluble core-shell quantum dot (QD) capped with thiolated ligand is used in this study for application in glucose sensing. These QDs were prepared in house based on hot injection technique. The ZnS shell at the outer surface of CdSe core QDs is made via specific process namely, SILAR (successive ionic layer adsorption and reaction). The distribution, morphology and optical characteristics of prepared core shell QDs have been assessed by transmission electron microscopy (TEM) and spectrofluorescence, respectively. The results show that the mean particle size of prepared QDs is in the range of 10-12 nm and the optimum emission condition was displayed at 620nm. The prepared CdSe/ZnS core shell QDs were modified by utilizing six organic ligands L-cysteine, L-histidine, thio-glycolic acid (TGA), mercapto-propionic acid (MPA), mercapto-succinic acid (MSA) and mercapto-undecanoic acid (MUA) at room temperature through a ligand-exchange procedure. This ligand exchange process was chosen in order to produce a very dense water solubilizing agent the QDs surrounding surface. The result shows that CdSe/ZnS capped with thioglycolic acid (CdSe/ZnS-TGA) exhibit the strongest fluorescence emission; therefore it was used in advanced sensing application for the detection of glucose. The highly active CdSe/ZnS-TGA was then interacted with Glucose Oxidase enzyme (GOx) and horseradish peroxidase enzyme (HRP). In this study, determination of glucose level is depending on the QDs fluorescence intensity quenching effect, which is correlated to reaction of the conjugated enzyme-QDs. In the presence of 0.1 mM glucose, fluorescence intensity of the bioconjugate QDs was quenched about 12, 000 a.u. This bioconjugated GOX/HRP/QDs-capped TGA was further analyzed with known concentrations of glucose. Quenched fluorescence intensity was proportionate with glucose concentration. The resultant GOX/HRP/QDs-capped TGA system can be a suitable platform for glucose determination in real samples.

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Current Progress in Nanotechnology Applications for Diagnosis and Treatment of Kidney Diseases

Lee

Bae

et al. 2015

Adv Healthcare Materials

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Significant progress has been made in nanomedicine, primarily in the form of nanoparticles, for theranostic applications to various diseases. A variety of materials, both organic and inorganic, have been used to develop nanoparticles with promise to achieve improved efficacy in medical applications as well as reduced systemic side effects compared to current standard of care medical practices. In particular, this article highlights the recent development and application of nanoparticles for diagnosing and treating nephropathologies.

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A highly sensitive system for urea detection by using CdSe/ZnS core-shell quantum dots

Cited by 142 publications

References 17 publications

Use of Water Soluble and Phosphorescent MPA Capped CdTe Quantum Dots for Detection of Urea

Use of Water Soluble and Phosphorescent MPA Capped CdTe Quantum Dots for Detection of Urea

Cdse/Zns Capped Thiolate for Application in Glucose Sensing

Current Progress in Nanotechnology Applications for Diagnosis and Treatment of Kidney Diseases

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