A highly sensitive quantification method for 12 plant toxins in human serum using liquid chromatography tandem mass spectrometry with a quick solid-phase extraction technique

Taniguchi, Masaru; Minatani, Tomiaki; Miyazaki, Hitoshi; Tsuchihashi, Hitoshi; Zaitsu, Kei

doi:10.1016/j.jpba.2020.113676

Cited by 8 publications

(12 citation statements)

References 27 publications

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“…A Nexera X2 LC system coupled with LCMS-8060 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan) was used for quantitative analysis. Based on our previous study [ 6 ], chromatographic separation was achieved using a Capcell Pak Inert ADME column (2.1 mm i.d. × 150 mm, particle size: 3 µm, Cat.No.95003, OSAKA SODA, Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Institute of Cancer Research (ICR) mice (male, 8 weeks old, 34.0–37.8 g) were purchased from Japan SLC Inc (Hamamatsu, Japan). According to our previous study [ 6 ], protoveratrine A (25 µg/weight kg) or colchicine (10 mg/weight kg) were administered intraperitoneally to mice ( n = 3) using a 25 gage needle-disposable syringe (Terumo Corporation, Tokyo, Japan). After 5 min of their administration, blood samples were collected from their abdominal aorta using a 25 gage needle-disposable syringe (Terumo Corporation) under isoflurane anesthesia using an inhalation apparatus (NARCOBIT-E, Natsume Seisakusho, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…To investigate the causative substances in such acute intoxication cases, highly sensitive and selective instruments are necessary for detecting even trace amounts of alkaloids in biological samples, such as blood. To meet these demands, liquid chromatography–tandem mass spectrometry (LC/MS/MS) is used to identify alkaloids in biological samples even in clinical laboratories, and the preparation of biological samples is essential [ 3 – 6 ]. However, analytical skills in sample preparation for biological samples are required to obtain accurate results since the sample preparation is generally manually operated, such as extraction and evaporation.…”

Section: Introductionmentioning

confidence: 99%

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Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Taniguchi¹,

Takamura

Watanabe

et al. 2022

Chromatographia

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In this study, we developed an easily operable quantification method for 21 plant-derived alkaloids in human serum by automatic sample preparation and liquid chromatography–tandem mass spectrometry. We designed to perform parallel sample preparation by a developed apparatus, which increased sample throughput. We conducted an automatic sample preparation through de-proteinization with 0.1% formic acid in methanol and achieved recovery rates of 89–107% (2.0–14% RSD) for all targeted analytes, demonstrating its high repeatability. The method validation results were satisfactory as follows: the linearity ( r 2 ) of each calibration curve ranged from 0.978 to 1.000; the inter- and intra-day accuracies were 89.0–125% and 82.1–110%, respectively; the inter- and intra-day precisions were below 13% and 10%, respectively. Additionally, the lower limits of detection and quantification were 0.0044–0.047 and 0.013–0.14 ng/mL, respectively. Finally, the developed method was applied to pseudo-protoveratrine A poisoning serum and pseudo-colchicine poisoning serum, which were prepared by diluting acute-poisoning mice serum with human serum. Our method successfully quantitated protoveratrine A (0.15–0.25 ng/mL) and colchicine (4.8–6.0 ng/mL). Thus, our method is essential for prompt clinical treatment and critical care on patient in acute intoxication cases caused by plant-derived alkaloids. Supplementary Information The online version contains supplementary material available at 10.1007/s10337-022-04212-5.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Taniguchi¹,

Takamura

Watanabe

et al. 2022

Chromatographia

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“…The cevanine alkaloids are defined by the presence of a six-membered E-ring, being highly hydroxylated, and a hemiketal linkage between C4 and C9 [6]. This compound is primarily recognized as one of the major alkaloids contributing to the cardiotoxic effects from Veratrum poisoning [15,27,31,84]. Veratridine has been identified as an agonist of voltage-gated sodium ion channels [85].…”

Section: Veratridinementioning

confidence: 99%

Veratrum parviflorum: An Underexplored Source for Bioactive Steroidal Alkaloids

Seale

McDougal

2022

Molecules

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Plants of the Veratrum genus have been used throughout history for their emetic properties, rheumatism, and for the treatment of high blood pressure. However, inadvertent consumption of these plants, which resemble wild ramps, induces life-threatening side effects attributable to an abundance of steroidal alkaloids. Several of the steroidal alkaloids from Veratrum spp. have been investigated for their ability to antagonize the Hedgehog (Hh) signaling pathway, a key pathway for embryonic development and cell proliferation. Uncontrolled activation of this pathway is linked to the development of various cancers; most notably, basal cell carcinoma and acute myeloid leukemia. Additional investigation of Veratrum spp. may lead to the identification of novel alkaloids with the potential to serve as chemotherapeutics. V. parviflorum is a relatively uncommon species of Veratrum that resides in the southeastern regions of North America. The phytochemical profile of this plant remains largely unexplored; however, bioactive steroidal alkaloids, including cyclopamine, veratramine, veratridine, and verazine were identified in its extract. The structural elucidation and bioactivity assessment of steroidal alkaloids in lesser abundance within the extract of V. parviflorum may yield potent Hh pathway inhibitors. This review seeks to consolidate the botanical and phytochemical information regarding V. parviflorum.

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“…Solid-phase extraction (SPE) is an efficient sample preparation technology [16][17][18], which is currently used for enrichment and separation of trace target analytes, especially in complex sample matrices. It combines separation and concentration using different solvents to selectively clean impurities or elute target substances with high purity.…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted solid‐phase extraction of Chikusetsu saponin IVa from Panacis majoris Rhizoma

Yang

Sun

et al. 2021

J of Separation Science

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As the main active component of Panacis majoris Rhizoma, Chikusetsu saponin IVa has the activity of anti-oxidation, anti-inflammatory pain, and so on.Obtaining high purity Chikusetsu saponin IVa by simple purification steps is a prerequisite for its deep development. In this paper, the separation and purification of Chikusetsu saponin IVa were studied by molecular imprinting technique. By ultraviolet and visible spectrophotometry and computer molecular simulation, it was concluded that water-soluble 3-(2-carboxyethyl)-1-vinylimidazolium bromide ionic liquid was the best functional monomer compared with acrylic acid and acrylamide. The molecularly imprinted polymers were prepared by precipitation polymerization at 60°C with Chikusetsu saponin IVa as template molecule, 3-(2-carboxyethyl)-1-vinylimidazolium bromide as functional monomer, ethylene glycol dimethacrylate as cross-linker, 2, 2'-azobisisobutyronitrile as initiator, and ethanol as porogen. The properties of molecularly imprinted polymers were studied by scanning electron microscopy, Fourier transform infrared spectroscopy, thermo-gravimetric analysis, nitrogen adsorption/desorption isotherm, and X-ray photoelectron spectroscopy. The maximum adsorption capacity was 171.33 mg/g, and the imprinting factor was 2.6. Finally, the polymers can be successfully used in the purification of Chikusetsu saponin IVa from Panacis majoris Rhizoma through a simple procedure, the content was significantly increased. The recoveries of the spiked samples for the CS-IVa ranged from 94.05 to 99.95% with relative standard deviation values lower than 2.67%. The results showed that the polymers demonstrated good adsorption capacity for Chikusetsu saponin IVa. Meanwhile, the polymers showed great stability and reusability during the application.

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A highly sensitive quantification method for 12 plant toxins in human serum using liquid chromatography tandem mass spectrometry with a quick solid-phase extraction technique

Cited by 8 publications

References 27 publications

Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Veratrum parviflorum: An Underexplored Source for Bioactive Steroidal Alkaloids

Molecularly imprinted solid‐phase extraction of Chikusetsu saponin IVa from Panacis majoris Rhizoma

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