A highly sensitive aptasensor for OTA detection based on hybridization chain reaction and fluorescent perylene probe

Wang, Bin; Wu, Yuanya; Chen, Yanfen; Weng, Bo; Xu, Liqun; Liu, Changming

doi:10.1016/j.bios.2016.02.062

Cited by 74 publications

(29 citation statements)

References 24 publications

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“…Plotan et al [124] applied an innovatively biochip array technology to multi-mycotoxin semi-quantitative screening in a large variety of feed ingredients, obtaining an overall average recovery of 104%. An optical aptasensor was developed based on the hybridization chain reaction amplification strategy and fluorescent perylene probe/DNA composites for ultrasensitive detection of OTA [125]. The application to corn samples demonstrated the feasibility and potential of the proposed enzyme-free amplification method in the practical applications of agricultural products.…”

Section: Mycotoxin Determination Methodsmentioning

confidence: 99%

Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods

Pereira

Cunha

Fernandes

2019

Toxins

157

105

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Today, we have been witnessing a steady tendency in the increase of global demand for maize, wheat, soybeans, and their products due to the steady growth and strengthening of the livestock industry. Thus, animal feed safety has gradually become more important, with mycotoxins representing one of the most significant hazards. Mycotoxins comprise different classes of secondary metabolites of molds. With regard to animal feed, aflatoxins, fumonisins, ochratoxins, trichothecenes, and zearalenone are the more prevalent ones. In this review, several constraints posed by these contaminants at economical and commercial levels will be discussed, along with the legislation established in the European Union to restrict mycotoxins levels in animal feed. In addition, the occurrence of legislated mycotoxins in raw materials and their by-products for the feeds of interest, as well as in the feeds, will be reviewed. Finally, an overview of the different sample pretreatment and detection techniques reported for mycotoxin analysis will be presented, the main weaknesses of current methods will be highlighted.

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Section: Mycotoxin Determination Methodsmentioning

confidence: 99%

Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods

Pereira

Cunha

Fernandes

2019

Toxins

157

105

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“…Recently, a series of aptamer-based methods for detecting OTA has been reported. These assays mainly include fluorescence [ 27 , 28 ], electrochemical assays [ 29 , 30 ], colorimetric analysis [ 31 , 32 , 33 ], nanomaterials [ 34 , 35 ], and photoelectrochemical immunosensors [ 36 ]. Thioflavin T (ThT), which is a commercially available water-soluble fluorogenic dye, has been demonstrated to selectively bind to G-quadruplexes.…”

Section: Introductionmentioning

confidence: 99%

Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe

Zhao

et al. 2018

Toxins

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Ochratoxin A (OTA) is one of the most common mycotoxins contaminating feed and foodstuffs. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, a fast and sensitive fluorescence aptamer biosensor has been proposed for the OTA assay. In the absence of OTA, the OTA aptamer can form a G-quadruplex structure with thioflavin T (ThT) dye, which results in increased fluorescence. After joining OTA, OTA aptamer combines with OTA and the G-quadruplex can be formed. Only faint fluorescence was finally observed when ThT weakly reacts with the quadruplex. Through this test method, the entire reaction and analysis process of OTA can be completed in 10 min. Under optimal experimental conditions (600 nM OTA-APT, 7 μM ThT, and 3 min incubation time), this proposed assay has a good limit of detection (LOD) of 0.4 ng/mL and shows a good linear relationship within the range of 1.2–200 ng/mL under the best experimental conditions. This method has a high specificity for OTA relative to Ochratoxin B (23%) and Aflatoxin B1 (13%). In addition, the quantitative determination of this method in real samples has been validated using a sample of red wine supplemented with a range of OTA concentrations (1.2 ng/mL, 12 ng/mL, and 40 ng/mL) with recoveries of 96.5% to 107%.

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“…It ranged from 1.0 to 20 pM with a detection limit of 0.1 pM, indicating that this current aptasensor was highly sensitive during the analysis of the OTA. Moreover, the practicality of this sensing strategy was validated through the successful detection of OTA levels in corn samples (Wang et al, 2016b).…”

Section: Fluorescent Aptasensor For Otamentioning

confidence: 99%

Aptamer-Based Biosensor for Detection of Mycotoxins

et al. 2020

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Mycotoxins are a large type of secondary metabolites produced by fungi that pose a great hazard to and cause toxic reactions in humans and animals. A majority of countries and regulators, such as the European Union, have established a series of requirements for their use, and they have also set maximum tolerance levels. The development of high sensitivity and a specific analytical platform for mycotoxins is much in demand to address new challenges for food safety worldwide. Due to the superiority of simple, rapid, and low-cost characteristics, aptamer-based biosensors have successfully been developed for the detection of various mycotoxins with high sensitivity and selectivity compared with traditional instrumental methods and immunological approaches. In this article, we discuss and analyze the development of aptasensors for mycotoxins determination in food and agricultural products over the last 11 years and cover the literatures from the first report in 2008 until the present time. In addition, challenges and future trends for the selection of aptamers toward various mycotoxins and aptasensors for multi-mycotoxins analyses are summarized. Given the promising development and potential application of aptasensors, future research studies made will witness the great practicality of using aptamer-based biosensors within the field of food safety.

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A highly sensitive aptasensor for OTA detection based on hybridization chain reaction and fluorescent perylene probe

Cited by 74 publications

References 24 publications

Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods

Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods

Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe

Aptamer-Based Biosensor for Detection of Mycotoxins

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