Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe

Wu, Kefeng; Ma, Changbei; Zhao, Han; He, Hui; Chen, Hanchun

doi:10.3390/toxins10050198

Cited by 51 publications

(25 citation statements)

References 42 publications

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“…The aptamer based biosenor showed low LOD (0.4 ng/mL) and good LRD of 1.2-200 ng/mL, which provide promising detecting approach for mytoxins in different foodstuff [98]. Aptamer antibody pair-based lateral flow assays using aptamer-coated AuNPs biosensor were employed to detect cholera toxin with low LOD of 0.6 ng/mL, thus offering a promising tool for the phathogen toxins [99].…”

Section: Recent Advances In Detecting Toxins By Aptasensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

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Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.Key Contribution: This article reviews systematically the toxicity of marine toxins, the development of aptarmer-based biosensors, and progress in the marine toxin detection by aptasensors.Toxins 2020, 12, 1 2 of 22 selection technology and biosensor fabrication technology offer a new solution for the detection of marine toxins with high efficiency and sensitivity [13][14][15][16][17].Marine toxins are a class of small molecular compounds mainly produced by red tide algae. In recent years, with the aggravation of environmental pollution, human deaths due to accidental ingestion of toxic shellfish are also often reported [18][19][20][21]. At present, the main methods for the detection of marine toxins include mouse bioassay (MBA) [22], enzyme-linked immunosorbent assay (ELISA) [23][24][25], high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), etc. [26][27][28]. However, these traditional methods have some drawbacks including the requirement of a technician, poor repeatability, expensive equipment, and issues related to animal ethics [22]. As a new detection technology, the biosensor is a kind of analysis systems using cell molecules and other bio-materials as sensitive elements, combined with a secondary sensor to detect a variety of chemicals by cascade amplified signal [29]. Because of their advantages of simple operation, high speed, high sensitivity, miniaturization, and easy automation, biosensors have been widely used in different fields including cell physiology [30], drug screening [31,32], food safety detection [33], disease diagnosis [34], etc.Aptamer, meaning "to fit", is a kind of oligonucleotide which can bind to target molecules with high selectivity and affinity. On account of the existence of aptamers in nature and the po...

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Section: Recent Advances In Detecting Toxins By Aptasensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

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“…Second, highly stable aptamers with prominent target diversity can be easily synthesized at low cost. Owing to these outstanding merits, several aptamer-based methods for OTA determination have been developed, such as electrochemistry- [24,25], fluorescence- [26,27], and colorimetry-based assays [28,29]. The first OTA aptamer synthesized by Cruz-Aguado for OTA detection was reported in 2008 and this aptamer has been widely used in the construction of many biosensors [30].…”

Section: Introductionmentioning

confidence: 99%

Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease

Zhao

Xiong

Yan

et al. 2020

Toxins

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In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJf exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA-apt). After optimizing the experimental conditions, the present aptamer-based sensing system can exhibit excellent fluorescent response in the OTA assay, with a limit of detection of 0.07 ng/mL. In addition to signal amplification, this strategy is also highly specific for other interfering toxins. Furthermore, this aptasensor can be reliably used for assessing red wine samples spiked with different OTA concentrations (2.4, 6 and 20 ng/mL). The proposed assay plays an important role in the field of food safety and can be transformed for detecting other toxins by replacing the sequence that recognizes the aptamer.

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“…At this time, the first excited state energy is the smallest and the quantum yield is the lowest, so the fluorescence intensity is very low. While ThT is combined with the G‐quadruplex, ring rotation is suppressed by steric hindrance, so fluorescence is restored . However, ThT is able to induce a potential nucleotide sequence to form a G‐quadruplex structure, which has an effect on the structure and function of nucleic acids and limits its application in kinetic detection.…”

Section: Introductionmentioning

confidence: 99%

A Lead (II) Ion Sensor Based on Selective Recognition of G‐quadruplex for Ethyl‐substitutive Thioflavin T

et al. 2019

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ThTÀ E (ethyl-substitutive Thioflavin T), a derivative of Thioflavin T (ThT), is capable of inducing a guanine -rich nucleic acid sequence that forms a parallel G-quadruplex under certain conditions and exhibits significant fluorescence enhancement. In this work, we used Pb 2 + to induce parallel G-quadruplex into anti-parallel G-quadruplex to reduce fluorescence of ThTÀ E and found a simple Pb 2 + detection method. Through this approach, Pb 2 + in aqueous solutions can be detected at 0. 76 nM with fluorescence spectrometry in a facile way, with high selectivity against other metal ions. These results indicate the method for detecting Pb 2 + is simple, sensitive, specific and label-free.

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Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe

Cited by 51 publications

References 42 publications

Marine Toxins Detection by Biosensors Based on Aptamers

Marine Toxins Detection by Biosensors Based on Aptamers

Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease

A Lead (II) Ion Sensor Based on Selective Recognition of G‐quadruplex for Ethyl‐substitutive Thioflavin T

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