A highly selective fluorescent probe for hypochlorite and its endogenous imaging in living cells

Abstract

An oxime based fluorescent probe has been designed and synthesized, which detects free as well as enzymatically generated hypochlorite with a low detection limit and high sensitivity. In addition, the probe was successfully utilized for the monitoring of endogenously produced hypochlorite in LPS stimulated cell lines, C6 glioma and BV2 microglia.

A Highly Selective Fluorescent Probe for Detection of Hydrogen Sulfide in Living Systems: In Vitro and in Vivo Applications

“…This indicates the fast response of probe 3 towards H 2 S (Figure S17 in the Supporting Information). The detection limit of probe 3 for H 2 S was as low as 0.13 μ m (Figure S18 in the Supporting Information), which was sufficiently low for monitoring H 2 S levels in biological systems. Furthermore, to demonstrate the efficiency of probe 3 for quantitative detection of H 2 S, we treated probe 3 with different concentrations of H 2 S to obtain a standard curve of emission intensity versus H 2 S concentration and regression analysis was performed (Figure S19 in the Supporting Information).…”

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

“…Toward this end, there have a number of fluorescent probes for HOCl been developed and used in living systems. But most of them are based on changes of the intensity, [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] which are limited to the quantification of HOCl due to the fluorescence intensity may be influenced by some external factors such as the concentration of probe, test temperature and instrument performance. Some small molecular based ratiometric HOCl probes have been constructed successfully.…”

Ratiometric Fluorescent Detection of ClO− Based on Dual‐Emission F1‐Rubpy@Nanoscale Metal‐Organic Frameworks

“…ClO − plays an essential role as defense layer and immune layer in biosystem due to its good bactericidal performance ,. Endogenic ClO − mainly locates in the acidic lysosome of macrophages in which a lower concentration can prompt phagocyte to destroy invading pathogens ,. However, excessive amounts of ClO − may destroy the body‘s defenses against disease thus cause tissue damage and certain diseases including atherosclerosis, liver injury, rheumatoid and cancer .…”

“…[1,2] Endogenic ClO À mainly locates in the acidic lysosome of macrophages in which a lower concentration can prompt phagocyte to destroy invading pathogens. [3,4] However, excessive amounts of ClO À may destroy the body's defenses against disease thus cause tissue damage and certain diseases including atherosclerosis, liver injury, rheumatoid and cancer. [5][6][7][8][9][10][11][12][13][14] Thus, monitoring of ClO À is of important significance.…”