A high sensitive visible light-driven photoelectrochemical aptasensor for shrimp allergen tropomyosin detection using graphitic carbon nitride-TiO2 nanocomposite

Tabrizi, Mahmoud Amouzadeh; Shamsipur, Mojtaba; Saber, Reza; Sarkar, Saeed; Ebrahimi, Vahid

doi:10.1016/j.bios.2017.06.040

Cited by 83 publications

(11 citation statements)

References 42 publications

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“…Aptamers have unique adaptabilities for different platforms and signaling modalities. For instance, researchers developed a novel and label‐free transporter system which is composed of aptamer and nanomotor for the conveying of Human Promyelocytic Leukemia Cells (HL‐60) [28]. In this study, nanomotors were formed of manganese oxide nanosheets and polyethyleneimine decorated with nickel/gold nanoparticles (MnO 2 −PEI/Ni/Au).…”

Section: Aptasensors For Biological Analytes Detectionmentioning

confidence: 99%

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Topkaya

Çetin²

2020

Electroanalysis

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Aptamers are short length, single‐stranded DNA or RNA affinity molecules which interact with any desired targets such as biomarkers, cells, biological molecules, drugs or chemicals with high sensitivity. They have been extensively employed for medical applications due to having more advantages than the antibodies such as easier preparation and modification, higher stability, lower batch‐to‐batch variability and cost. Moreover, aptamers can be easily integrated efficiently with sensors, biosensors, actuators and other devices. In this review article, different applications of aptamers for biological and chemical molecules detection within the scope of electrochemical methods were presented with recent studies. In addition, the present status and future perspectives for highly‐effective aptasensors for specific and selective analyte detection were discussed. As in stated throughout the review, combining of extraordinary properties of aptamers with the electrochemical‐based biosensors could have improved the sensitivity of the assay and reduced limit of detection.

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Section: Aptasensors For Biological Analytes Detectionmentioning

confidence: 99%

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Topkaya

Çetin²

2020

Electroanalysis

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“…The antigen–antibody reaction is the basis of immunoanalysis in clinical laboratories, and modern immuno-labeling techniques using highly sensitive substances as labels have further broadened the scope of immunological techniques. In recent years, oligonucleotide aptamers have exhibited high affinity and specificity, as well as high speed in selection, synthesis, and scale-up, which has attracted great attention in terms of diagnosis and therapeutics [ 37 , 38 , 39 ]. These specific MREs are firstly immobilized on the transducer’s surface as capture probes, and the target proteins can then be specifically recognized by capture probes, linking to the interface of biosensors.…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

“…In order to determine comprehensive design guidelines for more advanced PEC sensors, the previous review categorized recent PEC biosensor examples into three signaling principles [ 53 ], i.e., reactant determinants, electron transfer, and energy transfer. To further increase the photocurrent, many nanomaterials and nanostructures were incorporated into the construction of PEC biosensors to accelerate charge transfer [ 38 , 51 , 54 , 55 , 56 ] and increase the accessible surface [ 57 ].…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

“…These nanohybrids highly improved sensing sensitivity and showed results comparable to those attained via ELISA methods. A visible-light-driven PEC method for the detection of shrimp allergen tropomyosin was constructed using g-C 3 N 4 and TiO 2 as photoactive nanomaterials [ 38 ]. Ascorbic acid worked as an electron donor, and Ru(NH 3 ) 6 3+ was adsorbed on the specific aptamer to enhance the photocurrent signal ( Figure 6 ).…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

“…Inset: Time-based photocurrent response curves in the absence ( a ) and presence ( b ) of tropomyosin. Reproduced from [ 38 ] with permission from Elsevier.…”

Section: Figurementioning

confidence: 99%

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Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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