A high sensitive electrochemical nanoimmunosensor based on Fe3O4/TMC/Au nanocomposite and PT-modified electrode for the detection of cancer biomarker epidermal growth factor receptor

Omidfar, Kobra; Darzianiazizi, Maedeh; Ahmadi, Anita; Daneshpour, Maryam; Shirazi, H R Ghafarian

doi:10.1016/j.snb.2015.07.021

Cited by 46 publications

(15 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hysteresis curves in Figure S2B (Supporting Information File 1) show that the saturation magnetization of naked magnetic NPs (70.62 emu/g) has decreased after coating with TMC polymer and gold NPs. However, the zero remanence and coercivity values indicated the superparamagnetic nature of gold–magnetic NPs with an acceptable VSM value (59.05 emu/g) [20]. …”

Section: Resultsmentioning

confidence: 99%

“…It is claimed that the electrode surface may be damaged at high potentials, whereas low potentials is too week to oxidize gold. Therefore, a constant potential of 1.3 V was selected according to literatures [20] and the DPV analysis was carried out with different peroxidation times (20–120 s). The experiments were then performed at different potentials (1–1.5 V).…”

Section: Resultsmentioning

confidence: 99%

“…The highly flexible nature of these devices allows for applying various modifications to provide ultrasensitive quantitative measurements of specific biomolecules and even simultaneous evaluation of multiple analytes [19]. In addition, some novel approaches including the use of nanomaterials [20–22], semi-conductive/conductive polymers [23], and enzymatic strategies [24–26] have been recently employed to improve the detection sensitivity and also the stability of the electrochemical biosensors. It has been shown that these approaches can strongly amplify the signal, extend the linear detection range, and lower the detection limit of the assay [19].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

Daneshpour¹,

Omidfar²,

Ghanbarian³

2016

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

Gastric cancer (GC) is the second leading cause of cancer-related deaths all over the world. miR-106a is a circulatory oncogenic microRNA (miRNA), which overexpresses in various malignancies, especially in GC. In this study, an ultrasensitive electrochemical nanobiosensor was developed for the detection of miR-106a using a double-specific probe methodology and a gold–magnetic nanocomposite as tracing tag. The successful modification of the electrode and hybridization with the target miRNA were confirmed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) methods. Differential pulse voltammetry (DPV) was used for quantitative evaluation of miR-106a via recording the reduction peak current of gold nanoparticles. The electrochemical signal had a linear relationship with the concentration of the target miRNA ranging from 1 × 10−3 pM to 1 × 103 pM, and the detection limit was 3 × 10−4 pM. The proposed miRNA-nanobiosensor showed remarkable selectivity, high specificity, agreeable storage stability, and great performance in real sample investigation with no pretreatment or amplification. Consequently, our biosensing strategy offers such a promising application to be used for clinical early detection of GC and additionally the screen of any miRNA sequence.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

Daneshpour¹,

Omidfar²,

Ghanbarian³

2016

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Immunoassay is another approach to protein sensing. A recognized element, such as a primary antibody, is fixed to the surface of an electrode in a typical sandwich immunoassay to capture the specific analyte (Omidfar et al, 2015). Following that, a secondary antibody conjugated to an enzyme, such as horseradish peroxidase (HRP), is added to the solution to bind the antigen (analyte).…”

Section: Protein Constituentsmentioning

confidence: 99%

Advances in electrochemical biosensors for the detection of common oral diseases

Han

Liu

Guan

et al. 2023

Preprint

View full text Add to dashboard Cite

With the advancement of society and people’s knowledge of oral health, the prevention of oral illnesses such as dental caries, periodontal disease, oral cancer, oral mucosal disease, salivary gland disease, and so on has become the primary focus of current health status and treatment. Although treatment for these diseases has focused on removing lesions or inhibiting progression, early detection has always been the focus of clinical research. And the non-invasive diagnosis is now the primary goal of clinical diagnosis. Meanwhile, the electrochemical approach can satisfy this need while also achieving a speedy and reliable diagnosis, giving patients a more comfortable option. And noninvasive detection of specific diseases can be achieved by detecting markers carried in body fluids. Therefore, in the context of disease, pathophysiological progress and electrochemical-assisted diagnosis form a close relationship, providing a basis for the detection of oral disease related indicators. As a result, this review comprehensively summarizes the detection and diagnosis of common oral diseases using electrochemical sensing technology and provides important insights for the development of electrochemical biosensors for the early detection of common oral diseases.

show abstract

“…Due to the overexpression of EGFR in many aggressive cancer types, it is presented as a significant biomarker which provides this opportunity for early cancer diagnosis and thereby decreasing the morbidity and mortality associated with advanced disorder. The presented electrochemical biosensor showed a linear response in the range of concentration from 0 to 1000 pg/mL and a detection limit as low as 0.05 pg/mL [ 7 ].…”

Section: Screen Printing (Electrochemical) and Lfa (Optical) Poc Biosmentioning

confidence: 99%

Point-of-care biosensors in medicine: a brief overview of our achievements in this field based on the conducted research in EMRI (endocrinology and metabolism research Institute of Tehran University of medical sciences) over the past fourteen years

Omidfar

Ahmadi

Syedmoradi

et al. 2020

J Diabetes Metab Disord

Self Cite

View full text Add to dashboard Cite

The growing demand of diagnostic tools with enhanced analytical characteristics in term of sensitivity, selectivity, and low response time has encouraged researches to conduct their research towards development of point-of-care (POC) biosensors. POC diagnostic devices are powerful tools for detection, diagnosis, and monitoring of diseases at its initial stage. The above characteristics encouraged us to conduct active multidisciplinary and collaborative research oriented towards the design and development of POC sensing systems. Here, we present a brief overview of our recent achievement in the field of biomedical POC devices implemented in paper based microfluidic and screen printing electrodes and discuss the critical limitations that need to be surmounted to facilitate their translation into clinical practice in the future.

show abstract

A high sensitive electrochemical nanoimmunosensor based on Fe3O4/TMC/Au nanocomposite and PT-modified electrode for the detection of cancer biomarker epidermal growth factor receptor

Cited by 46 publications

References 45 publications

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

Advances in electrochemical biosensors for the detection of common oral diseases

Point-of-care biosensors in medicine: a brief overview of our achievements in this field based on the conducted research in EMRI (endocrinology and metabolism research Institute of Tehran University of medical sciences) over the past fourteen years

Contact Info

Product

Resources

About