A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis

Finkers-Tomczak, A.M.; Danan, Sarah; Dijk, T. van; Amelework, Beyene; Bouwman, L.I.; Overmars, H.A.; Eck, Herman J. van; Goverse, A.; Bakker, J.; Bakker, E.H.

doi:10.1007/s00122-009-1026-1

Cited by 36 publications

(26 citation statements)

References 37 publications

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“…Дополнительно изучали наличие у образцов выборки маркера TG432/RsaI 1900 гена Grp1, детерминирующего устойчивость к обоим видам цистообразующих нематод -G. pallida (патотипы Pa2, Pa3) и G. rostochiensis (патотип Ro5) (Finkers-Tomczak et al, 2009). Диагностический фрагмент маркера TG432/RsaI -1 900 п. о.…”

Section: Mas с маркерами генов устойчивости к цистообразующим нематодамunclassified

Molecular Screening of Potato Varieties Bred in the Northwestern Zone of the Russian Federаtion

Гавриленко¹,

Klimenko²,

Antonova³

et al. 2018

Vestn. VOGiS

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Increasing the resistance of varieties to diseases and pests is a priority in potato breeding. Many potato varieties created in the Leningrad Scientific Research Institute of Agriculture "Belogorka" are multi-species hybrids possessing complex resistance to the most spread pathogens in combination with a high plasticity, which determined their zoning in many regions of the Russian Federation. Potato varieties and hybrids created in different periods of time in this institute and in the Ltd Breeding company "LiGa" were included in marker-assisted selection with 22 markers of 14 R genes conferring extreme resistance to viruses PVY and PVX, resistance to cyst nematodes Globodera pallida (patotypes Pa2, Pa3) and G. rostochiensis (pathotype Ro1) and resistance to late blight. The data of molecular screening were compared with the results of the State Test of Varieties for Disease Resistance, as well as with other published sources. Markers of sterile and fertile cytoplasm types were also used in the study. Based on the results of molecular screening, varieties and hybrids with different combinations of markers of R genes conferring resistance to the most harmful pathogens (Ry sto , Ryf sto , Rx1, Rx2, Gro14, H1, Gpa2, R1, R3a, Rpiblb1, Rpisto1) were detected. Among them, genotypes with sterile D-, T-, W/gamma cytoplasmic types were selected. Analysis of their pollen fertility, crossability and pedigree data showed that breeders succeed in obtaining fertile forms with the D-and Задача повышения устойчивости сортов к болезням и вредителям является приоритетной для селекции картофеля. Многие сорта, созданные селекционерами северо-западной зоны Российской Федерации, являются многовидовыми гибридами, обладают ком-плексной устойчивостью к наиболее вредоносным патогенам в сочетании с высокой пластичностью, о чем свидетельствует их районирование в разных регионах нашей страны. В статье пред-ставлены результаты молекулярного скрининга сортов и гибридов, созданных в разные годы в ЛенНИИСХ «Белогорка» и в ООО Селек-ционная фирма «ЛиГа». Молекулярный скрининг проведен с ис-пользованием 22 маркеров 14 R-генов устойчивости к вирусам PVY и PVX, к цистообразующим нематодам -Globodera pallida (патотипы Pa2, Ра3) и G. rostochiensis (патотип Ro1) и к фитофторозу. Данные молекулярно-генетического анализа сопоставлялись с результа-тами Государственного испытания сортов на устойчивость к бо-лезням и с другими опубликованными источниками. В работе ис-пользованы также маркеры стерильных и фертильных типов цито-плазм. По результатам молекулярного скрининга выделены сорта и гибриды c разными сочетаниями маркеров R-генов (Ry sto , Ryf sto , Rx1, Rx2, Gro14, H1, Gpa2, R1, R3a, Rpiblb1, Rpisto1), обладающие D-, T-, W/gamma стерильными типами цитоплазм. Анализ фертиль-но сти пыльцы, результатов скрещиваний и данных родословных показал, что селекционерам удалось получить фертильные формы с D-и Т-типами цитоплазм, несущие генетический материал мекси-канских полиплоидных видов Solanum stoloniferum и S. demissum. Информация о наличии у гибридов и с...

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Section: Mas с маркерами генов устойчивости к цистообразующим нематодамunclassified

Molecular Screening of Potato Varieties Bred in the Northwestern Zone of the Russian Federаtion

Гавриленко¹,

Klimenko²,

Antonova³

et al. 2018

Vestn. VOGiS

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“…Gro1–4 is a member of the Gro1 locus, which confers nearly absolute resistance to all pathotypes of G. rostochiensis , and it is therefore considered a useful resistance gene [ 58 ]. Broad-spectrum resistance to G. rostochiensis and G. pallida is conferred by the Grp1 gene [ 59 ]. Only two G. rostochiensis resistance genes were characterized at the molecular level: Gpa2 from S. tuberosum ssp.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

et al. 2017

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BackgroundThe characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming.ResultsComparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode.ConclusionCombination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1193-1) contains supplementary material, which is available to authorized users.

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“…Good data quality should result in a striping pattern whereas error adds isolated spots. Thirdly, high resolution mapping of major-effect loci (Finkers-Tomczak et al 2009) is supported by a graphical analysis of the recombinants and their trait values, allowing one to zoom in on the remaining interval where candidate genes reside. Graphical genotyping is supported by software packages such as GGT (van Berloo, 1999;2008) or Flapjack ).…”

Section: Graphical Genotypingmentioning

confidence: 99%

Development and application of a 20K SNP array in potato

Vos¹

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A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis

Cited by 36 publications

References 37 publications

Molecular Screening of Potato Varieties Bred in the Northwestern Zone of the Russian Federаtion

Molecular Screening of Potato Varieties Bred in the Northwestern Zone of the Russian Federаtion

Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

Development and application of a 20K SNP array in potato

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