Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

Кочетов, А. В.; Glagoleva, Anastasiya Y.; Стрыгина, К. В.; Khlestkina, Elena K.; Gerasimova, S. V.; Ibragimova, S. M.; Shatskaya, Natalja V.; Vasilyev, Gennady V.; Afonnikov, D. A.; Shmakov, Nikolay A.; Antonova, Olga; Гавриленко, Т. А.; Alpatyeva, Natalia V.; Хютти, А. В.; Afanasenko, Olga

doi:10.1186/s12870-017-1193-1

Cited by 18 publications

(20 citation statements)

References 58 publications

(76 reference statements)

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“…Plant R gene products directly or indirectly mediate the recognition of pathogen effector proteins and trigger downstream signaling cascades in innate immunity [1,69]. However, screening for R gene variants conferring resistance to a specific pathogen or new pathogen races is complicated and labor-intensive [70]. In the present study, three RPS2 (BraA09g060910.3C, BraA09g060310.3C, and BraA09g060320.3C), one PBS1 (BraA03g005610.3C), and one RPS5 (BraA06g009350.3C) gene were up-regulated at 8 dpi ( Figure 6 and Supplementary Table S7).…”

Section: Up-regulation Of Disease Resistance Genes In the Late Stage mentioning

confidence: 99%

Transcriptome Arofile of Brassica rapa L. Reveals the Involvement of Jasmonic Acid, Ethylene, and Brassinosteroid Signaling Pathways in Clubroot Resistance

Piao

Zhan

et al. 2019

Agronomy

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Plasmodiophora brassicae is a protozoan pathogen that causes clubroot disease in cruciferous plants, particularly Chinese cabbage (Brassica rapa). A previous study identified a clubroot resistance gene (CRd) conferring race-specific resistance to P. brassicae. However, the defense mechanisms of B. rapa against virulent vs. avirulent P. brassicae are poorly understood. In this study, we carried out a global transcriptional analysis in the clubroot-resistant Chinese cabbage inbred line “85–74” carrying the CRd gene and inoculated with avirulent (LAB-4) or virulent (SCCD-52) P. brassicae. RNA sequencing showed that “85–74” responded most rapidly to SCCD-52 infection, and the number of differentially expressed genes was much higher in SCCD-52-treated as compared to LAB-4-treated plants (5552 vs. 304). Transcriptome profiling revealed that plant hormone signal transduction and plant–pathogen interaction pathways played key roles in the late stages of P. brassicae infection. Genes relating to the salicyclic acid (SA), jasmonic acid (JA)/ethylene (ET), and brassinosteroid (BR) signaling pathways were up-regulated relative to untreated plants in response to LAB-4 infection at 8, 16, and 32 days post-inoculation (dpi) whereas JA, ET, and BR signaling-related genes were not activated in response to SCCD-52, and SA signaling-related genes were up-regulated in both LAB-4 and SCCD-52, suggesting that SA signaling is not the key factor in host resistance to avirulent P. brassicae. In addition, genes associated with phosphorylation and Ca2+ signaling pathways were down-regulated to a greater degree following LAB-4 as compared to SCCD-52 infection at 8 dpi. These results indicate that effector-triggered immunity in “85–74” is more potently activated in response to infection with avirulent P. brassicae and that JA, ET, and BR signaling are important for the host response at the late stage of infection. These findings provide insight into P. brassicae pathotype-specific defense mechanisms in cruciferous crops.

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Section: Up-regulation Of Disease Resistance Genes In the Late Stage mentioning

confidence: 99%

Transcriptome Arofile of Brassica rapa L. Reveals the Involvement of Jasmonic Acid, Ethylene, and Brassinosteroid Signaling Pathways in Clubroot Resistance

Piao

Zhan

et al. 2019

Agronomy

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“…Blight resistance protein Rpi-blb2, TMV resistance protein N, Tospovirus resistance protein C, RPI protein, late blight resistance protein, Cc-nbs-lrr resistance protein, R3a disease resistance protein, Nbs-lrr resistance protein, and HJTR2GH1 protein were found expressed in the root transcriptome of Solanum phureja confronted with Globodera rostochiensis, Kochetov (2017). In wild potato relatives resistance genes were identified as Gro1-4, which confer resistance to all the pathotypes of G. rostochiensis (Paal et al 2004).…”

Section: Librariesmentioning

confidence: 95%

“…One of the more recent methods for identifying new genes is RNA Sequencing (RNA-seq) or massive transcriptome sequencing (Kochetov 2017). RNA-Seq has become instrumental to the discovery of new genes involved in resistance to various pathogens in plants (Wang et al 2016, Kong et al 2015.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiling against Globodera pallida in resistant and susceptible potato roots

et al. 2020

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Globodera pallida is a white potato cyst nematode present in the Andes, which causes huge losses to Peruvian farmers. An RNA-seq analysis allowed the identification of candidate genes that could mediate resistance against this pathogen. Two varieties, “María Huanca” (Solanum andigena) clone resistant (CIP 279142.12) and “Chimbina Colorada” (Solanum chaucha) (CIP 701013) clone susceptible to G. pallida, were used to identify differentially expressed genes. Total RNA from roots was extracted 72 hours post inoculation with second stage juveniles. Sequencing was done using the Illumina Hiseq 2500 platform. Reads were screened for quality issues and then mapped to the reference potato genome (clone DM1-3516 R44 v4.03). Here, we report 27717 and 27750 genes expressed in the resistant and susceptible variety respectively. The comparative analysis of expression identified 100 candidate genes. 91 genes were associated with resistance to G. pallida with Fold Change ≥ 2 (p <0.05). The remaining 9 R genes had Fold Change ≤ 1. We show differences in the expression of an NBS-LRR protein similar to Gro1-8, genes linked to late blight and TMV virus resistance.

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“…Phytohormones such as JA and SA not only function to regulate plant growth but also are involved in plant defense signaling pathways [4]. Genes that encode nucleotide-binding site-leucine-rich repeats (NBS-LRRs) are the predominant members of the R gene family, accounting for approximately 80% of the more than 140 cloned R genes [5]. In potato, Gpa2 is an R gene that encodes the GPA2 protein, which depends on the recognition specificity afforded by both amino acid 187 and the Gpa2 LRR domain and provides resistance against two Globodera pallida nematode populations (D383 and D372) [6].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiling of Solanum peruvianum LA3858 revealed a Mi-3-mediated hypersensitive response to Meloidogyne incognita

Jiang

Zhang

et al. 2020

BMC Genomics

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Background: The Mi-1 gene was the first identified and cloned gene that provides resistance to root-knot nematodes (RKNs) in cultivated tomato. However, owing to its temperature sensitivity, this gene does not meet the need for breeding disease-resistant plants that grow under high temperature. In this study, Mi-3 was isolated from the wild species PI 126443 (LA3858) and was shown to display heat-stable resistance to RKNs. However, the mechanism that regulates this resistance remains unknown. Results: In this study, 4760, 1024 and 137 differentially expressed genes (DEGs) were enriched on the basis of pairwise comparisons (34°C vs. 25°C) at 0 (before inoculation), 3 and 6 days post-inoculation (dpi), respectively. A total of 7035 DEGs were identified from line LA3858 in the respective groups under the different soil temperature treatments. At 3 dpi, most DEGs were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to plant biotic responses, such as "plant-pathogen interaction" and "plant hormone signal transduction". Significantly enriched DEGs were found to encode key proteins such as R proteins and heat-shock proteins (HSPs). Moreover, other DEGs were found to participate in Ca 2+ signal transduction; the production of ROS; DEGs encoding transcription factors (TFs) from the bHLH, TGA, ERF, heat-shock transcription factor (HSF) and WRKY families were highly expressed, which contribute to be involved into the formation of phytohormones, such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET), the expression of most was upregulated at 3 dpi at the 25°C soil temperature compared with the 34°C soil temperature.

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Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

Cited by 18 publications

References 58 publications

Transcriptome Arofile of Brassica rapa L. Reveals the Involvement of Jasmonic Acid, Ethylene, and Brassinosteroid Signaling Pathways in Clubroot Resistance

Transcriptome Arofile of Brassica rapa L. Reveals the Involvement of Jasmonic Acid, Ethylene, and Brassinosteroid Signaling Pathways in Clubroot Resistance

Transcriptomic profiling against Globodera pallida in resistant and susceptible potato roots

Transcriptomic profiling of Solanum peruvianum LA3858 revealed a Mi-3-mediated hypersensitive response to Meloidogyne incognita

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