A High Performance Liquid Chromatography Method for the Determination of Glycosaminoglycans in Human Blood

Gässler, Norbert; Reißner, C.; Janzen, Nils; Kähnert, Heike; Kleesiek, Knut

doi:10.1515/cclm.1993.31.8.503

Cited by 7 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding the sequence of application of the GAG lyases, recovery rates using the order employed in the present study proved to be superior to other methods that used hyaluronate lyase digestion as the first degradation step [23], since CA and DS can also be digested by hyaluronate lyase [24]. Comparison of the specific-enzyme digestion and HPLC analysis method with the commonly used dye reaction showed the higher recovery rate, sensitivity and reproducibility of the HPLC analysis.…”

Section: Discussionmentioning

confidence: 68%

“…Enzymic digestion and HPLC analysis of GAG compounds has been successfully applied in human articular cartilage [23] and in serum and plasma samples [24] using different chromatographic columns, such as strong anion-exchange and reversedphase columns. In this study we employed an aminopropyl column which is designed for disaccharide separation based on polar and hydrophobic interactions dependent upon the mobile phase used.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

HPLC Glycosaminoglycan Analysis in Patients with Graves' Disease

et al. 1997

View full text Add to dashboard Cite

1. Orbital accumulation of hydrophilic, interstitial glycosaminoglycans (GAGs) and subsequent expansion of retrobulbar tissue lead to the clinical manifestation of exophthalmos in patients with Graves' eye disease. 2. A highly specific method to determine the concentration and biochemical composition of different GAGs was developed in order to obtain a sensitive test system for the activity of the disease. By means of this method, GAG excretion in 24 h urine collections of 56 patients and 21 controls was analysed by precipitation with cetylpyridinium chloride and potassium acetate in ethanol, followed by sequential enzymic hydrolysis with chondroitin AC lyase, chondroitin ABC lyase and hyaluronate lyase, with HPLC analysis of the resulting alpha, beta-unsaturated disaccharides by anion-exchange chromatography. 3. Concentrations of GAG, chondroitin sulphate A (CA), dermatan sulphate (DS) and hyaluronic acid (HA) were determined in patients and controls, with high recovery rates [72.2 +/- 5.3%, mean +/- SEM; detection limit, 4.2 micrograms/l (0.01 mumol/l)], revealing marked differences in urinary concentrations of total GAG and HA, as well as an elevation of CA in patients compared with controls. 4. Method sensitivity was 0.86 for patients with active Graves' eye disease, and 0.87 for patients with untreated ophthalmopathy, whereas specificity was 1.0 for patients with inactive disease. Patients with increased GAG concentration responded well to steroids and/or orbital irradiation (before therapy: GAG, 111.49 +/- 40.32; CA, 59.58 +/- 21.34; DS, 25.05 +/- 8.12; HA, 26.88 +/- 11.63 mg/24 h; during therapy: GAG, 54.22 +/- 10.94; CA, 20.52 +/- 4.58; DS, 17.65 +/- 3.46; HA, 16.05 +/- 3.69 mg/24 h), whereas GAG excretion increased markedly 2-3 months after stopping prednisone therapy in patients with still active eye disease (GAG, 109.9 +/- 10.51; CA, 63.8 +/- 7.34; DS, 24.1 +/- 5.07; HA, 22.0 +/- 6.28 mg/24 h). 5. This sensitive method determines the nature of renally excreted GAGs, reflecting the aberrant synthesis pattern of fibroblasts in patients with Graves' disease.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

HPLC Glycosaminoglycan Analysis in Patients with Graves' Disease

et al. 1997

View full text Add to dashboard Cite

show abstract

“…The concentration of total glycosaminoglycans (GAGs) and chondroitin sulfate (CS) was assessed by High-performance liquid chromatography (HPLC) on Day 0, 1st week then at the 1st, 2nd, 3rd, 5th, and 7th months post-MIA-injection as modified according to Gässler et al 44 . Briefly; serum and synovial samples were dissolved with 1:1 a ratio for the sample to solvent (deionized water: acetonitrile 80:20%) and then centrifuged at 1957× g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis

Yassin

AbuBakr

Abdelgalil

et al. 2023

Sci Rep

View full text Add to dashboard Cite

One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers. OA was induced by a single intra-articular injection of 25 mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR-146b, miR-27b, TRAF-6, and COL10A1 gene expression. The results showed that the total GAGs and CS levels increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA and then decreased at the late stages (P < 0.05). In conclusion, both miR-146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA.

show abstract

“…The concentration of total glycosaminoglycans (GAGs) and chondroitin sulfate (CS) was assessed by High-performance liquid chromatography (HPLC) at Day 0, 1 st week, 1 st month, 2 nd months, 3 rd months,5 th months, and 7 th months post-MIA-injection as modi ed according to [44]. Brie y; serum and synovial samples were dissolved with deionized water: acetonitrile (80:20%) then centrifuged at 5000 rpm for 10 min.…”

Section: Biochemical Analysismentioning

confidence: 99%

Circulating microRNAs Signature as a promising epigenetic diagnostic biomarker of equine osteoarthritis

Yassin

AbuBakr

Abdelgalil

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers.Methods: OA was induced by a single intra-articular injection of 25mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR 146b, miR 27b, TRAF-6, and COL10A1 gene expression.Results: The results showed that the level of total GAGs and CS increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA then decreased at late stages (P<0.05).Conclusions: In conclusion, both miR146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA.

show abstract

A High Performance Liquid Chromatography Method for the Determination of Glycosaminoglycans in Human Blood

Cited by 7 publications

References 14 publications

HPLC Glycosaminoglycan Analysis in Patients with Graves' Disease

HPLC Glycosaminoglycan Analysis in Patients with Graves' Disease

Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis

Circulating microRNAs Signature as a promising epigenetic diagnostic biomarker of equine osteoarthritis

Contact Info

Product

Resources

About