Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis

Abstract

One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers. OA was induced by a single intra-articular injection of 25 mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR-146b, miR-27b, TRAF-6, and COL10A1 gene expression. The results showed that the total GAGs and CS levels increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA and then decreased at the late stages (P < 0.05). In conclusion, both miR-146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA.

Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses

“…Currently there are only few studies reporting expression of miRNAs in experimental and naturally occurring equine OA (54,(96)(97)(98). There is no consistency in the miRNAs identified as being differentially expressed in the studies, and it is therefore impossible at this stage to speculate about the validity of our or other experimental models in mimicking changes occurring in spontaneous equine OA.…”

Collagen type X expression and chondrocyte hypertrophic differentiation during OA and OS development

“…Sirtuin 1 (SIRT1) mediates MIAinduces OA pain by upregulating p53 expression, which makes targeting SIRT1/P53 pathway a possible new therapeutic option [98]. In a study of early biomarkers of OA induced OA in donkeys using MIA, significant up-regulation of miR-146b, miR-27b and Col10a1 expression in serum and synovial fluid was observed in early OA in this animal model [99]. It can be seen from the above studies that the MIA-induced OA model is often used in the pain research of OA, and it can be selected in the pain treatment.…”