A High-Performance Liquid Chromatographic Method for the Determination of 8-Oxo-7,8-dihydro-2′-deoxyguanosine in Urine from Man and Rat

Lengger, Christoph; Schöch, G.; Topp, Heinrich

doi:10.1006/abio.2000.4839

Cited by 32 publications

(21 citation statements)

References 21 publications

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“…Therefore the preparation of a urine sample for analysis by HPLC-ECD for the 8-OHdG content relies heavily on solid-phase extraction (SPE) technology, which allows for significant enrichment of the analyte. [49][50][51][52][53][54] If the analyte is 8-hydroxy-2'-deoxyguanosine -considered as a product of hydrolysis of cellular DNA -it is necessary to carry out hydrolysis of the DNA, and digestion with the appropriate enzymes: nuclease P1 and alkaline phosphatase. Nuclease P1 cuts the DNA, leading to the formation of the corresponding nucleosides.…”

Section: Direct Methodsmentioning

confidence: 99%

Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Dąbrowska¹,

Wiczkowski²

2017

Adv Clin Exp Med

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Under homeostatic conditions, an equilibrium state between amounts of free radicals formed and their scavenging is observed. Free radicals are destructive only when present in excess. Pathological changes within cells and tissues can result from a persistent excess of free radicals. Living organisms are increasingly exposed to oxidative stress, resulting in oxidative DNA modifications. One such modification is 8-hydroxy-2'-deoxyguanosine (8-OHdG). It is considered a biomarker of oxidative stress and oxidative DNA damage. It has been found both in physiological fluids and in cells. This paper presents methods found in the literature for determining 8-OHdG expression in various kinds of biological material -blood, urine or liver homogenates. Methods for determining the biomarker expression have been grouped into direct and indirect methods, and the various levels of 8-hydroxy-2'-deoxyguanosine that can be determined by the different techniques are presented. The basic pros and cons of the various techniques are also discussed.

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Section: Direct Methodsmentioning

confidence: 99%

Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Dąbrowska¹,

Wiczkowski²

2017

Adv Clin Exp Med

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“…Since the 8-oxoguanine residues are electroactive, a variety of electrochemical methods were reported on the electrooxidation of oxidative modified base, 8-oxoguanine (Oliveira Brett et al, 2000;Rebelo et al, 2004a) or oxidized nucleoside, 8-oxodeoxyguanosine (Langmaier et al, 2003), to study the oxidation mechanism on electrode surface. The high performance liquid chromatography with electrochemical detection (HPLC-ECD) and the capillary electrophoresis with amperometric detection (CE-ECD) (Weiss and Lunte, 2000;Inagaki et al, 2001) have been developed for quantification of 8-oxoguanine and 8-oxodeoxyguanosine (Bogdanov et al, 1999;Helbock et al, 1998;Kasai, 2003;Lengger et al, 2000;Svoboda and Kasai, 2004;Rebelo et al, 2004b). In addition, isotope dilution mass spectrometry (Holmberg et al, 1999), avidin and its analogues (Struthers et al, 1998) were also used for the analytical detection of 8-oxodeoxyguanosine and 8-oxoguanine.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical performance of 8-hydroxy-2′-deoxyguanosine and its detection at poly(3-methylthiophene) modified glassy carbon electrode

Jia

Wang

et al. 2007

Biosensors and Bioelectronics

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“…After the discovery of oxidized nucleotides, nucleosides and nucleobases as important biomarkers in cell extract and body fluids, high performance liquid chromatography with electrochemical detection has been implemented as efficient analytical method in the determination of these biomarkers [7,8]. Moreover, cyclic voltammetry (CV) has been used in numerous studies in order to estimate oxidation potentials and oxidation mechanisms of nucleotides, nucleosides and bases [9 -12].…”

Section: Introductionmentioning

confidence: 99%

On‐Line Electrochemistry/Electrospray Ionization Mass Spectrometry (EC/ESI‐MS) for the Generation and Identification of Nucleotide Oxidation Products

et al. 2010

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The oxidation behavior of DNA and RNA nucleotides is studied by an on-line set-up consisting of an electrochemical thin-layer cell (EC) directly coupled to electrospray ionization mass spectrometry (ESI-MS). This set-up allows the generation of nucleotide oxidation products in the electrochemical cell at increasing potentials. Moreover, the products are determined directly, without isolation or derivatization steps, by electrospray ionization time of flight mass spectrometry (ESI-ToF/MS). The dependence of the mass spectra on the applied potential is displayed as mass voltammograms. An advanced set-up, consisting of the electrochemical cell coupled to electrospray ionization tandem mass spectrometry (EC/ESI-MS/MS) allows further structure elucidation based on fragmentation experiments. The electrochemical conversion is performed using a boron doped diamond (BDD) working electrode, which is known to generate hydroxyl radicals at high potentials. The capability of the EC-MS system to generate highly relevant oxidation products which also occur upon oxidative damage in vivo is demonstrated in this study by the formation of well known biomarkers for DNA damage, including 2'-deoxy-8-oxo-guanosine 5'-monophosphate.

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A High-Performance Liquid Chromatographic Method for the Determination of 8-Oxo-7,8-dihydro-2′-deoxyguanosine in Urine from Man and Rat

Cited by 32 publications

References 21 publications

Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Electrochemical performance of 8-hydroxy-2′-deoxyguanosine and its detection at poly(3-methylthiophene) modified glassy carbon electrode

On‐Line Electrochemistry/Electrospray Ionization Mass Spectrometry (EC/ESI‐MS) for the Generation and Identification of Nucleotide Oxidation Products

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