Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Dąbrowska, Natalia; Wiczkowski, Andrzej

doi:10.17219/acem/43272

Cited by 54 publications

(38 citation statements)

References 45 publications

(59 reference statements)

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“…Finally, we investigated the involvement of oxidative stress and endoplasmic reticulum (ER) stress in the inhibitory effect by ipragliflozin on liver steatosis and fibrosis in FLS-ob/ob mice. Since 8-OHdG has been reported to be a hallmark of oxidative stress, 10 changed by the treatment with ipragliflozin. As representative ER stress markers, we measured hepatic mRNA levels of ATF3, CHOP, C-JUN and NUPR1 ( Table 2).…”

Section: Effects Of Ipragliflozin On Oxidative Stress and Endoplasmicmentioning

confidence: 99%

Protective Effects of Ipragliflozin, a Sodium-glucose Cotransporter 2 Inhibitor, on a Non-alcoholic Steatohepatitis Mouse Model

et al. 2019

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Background The options for the treatment of nonalcoholic steatohepatitis (NASH) are limited. We examined the effects of ipragliflozin, a sodium-glucose cotransporter 2 inhibitor, on the fatty liver Shionogi (FLS)-ob/ ob mice, a non-alcoholic steatohepatitis mouse model. Methods FLS-ob/ob male mice were treated with vehicle (n = 10) and ipragliflozin (n = 8). Serum metabolic markers, histopathology of the liver, hepatic cholesterol and triglyceride levels and hepatic mRNA levels related to fibrosis, lipid metabolism and endoplasmic reticulum (ER) stress were compared between the two groups. Results The body weight and hepatic cholesterol and triglyceride levels were significantly decreased in the ipragliflozin group compared with the control group. Hepatic steatosis and fibrosis were significantly ameliorated by the treatment with ipragliflozin. Hepatic infiltration of macrophage, expression levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) and hepatic mRNA levels of ER stress markers were not significantly modulated by the treatment with ipragliflozin. Conclusion Ipragliflozin can be a therapeutic option for patients with NASH. The precise mechanisms of action need to be clarified in future studies.

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Section: Effects Of Ipragliflozin On Oxidative Stress and Endoplasmicmentioning

confidence: 99%

Protective Effects of Ipragliflozin, a Sodium-glucose Cotransporter 2 Inhibitor, on a Non-alcoholic Steatohepatitis Mouse Model

et al. 2019

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“…Oxidative stress along with the disruption of phospholipid membranes were also reflected by a higher level of 8‐isoprostane in PC patients. In addition, we observed a possible damage to genetic information, which was indicated by a slight increase of 8‐hydroxyguanosine and 8‐hydroxydeoxyguanosine in PC patients. Although the differences within the leukotriene C/D/E 4 cascade were negligible, the activation of the patient immune system was expressed by the considerably increased level of leukotriene B 4 , the primary inflammation marker …”

Section: Resultsmentioning

confidence: 85%

Chiroptical spectroscopy and metabolomics for blood‐based sensing of pancreatic cancer

et al. 2018

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To enable the early diagnosis of pancreatic cancer, the search for and definition of reliable biomarkers remain a subject of great interest, with the specificity and sensitivity of the currently used biomarkers being below the required values. We tested a novel diagnostic approach for pancreatic cancer based on the specific molecular signature of blood plasma components. To acquire more detailed structural information, structure-sensitive chiroptical methods (electronic circular dichroism and Raman optical activity) were supplemented by conventional Raman and infrared spectroscopies. The obtained spectra were subsequently processed by linear discriminant analysis yielding high values of specificity and sensitivity. In addition, to monitor not only large biomolecules as potential biomarkers but also those of low molecular weight, we conducted an analysis of blood plasma samples by using metabolomics. The achieved results suggest a panel of promising biomarkers for a reliable detection of pancreatic cancer.

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“…26 The 8-OHdG/10 6 dG levels were studied in several pathological conditions such as cancer types, diabetes mellitus, arteriosclerosis, neurodegenerative disorders of the brain, and autoimmune diseases. 27 This is the first study to investigate the oxidative DNA damage in children with UDT. The 8-OHdG/10 6 dG level of the UDT group was significantly higher than those of the healthy control group.…”

Section: Discussionmentioning

confidence: 99%

Oxidative DNA Damage and NOX4 Levels in Children with Undescended Testes

2019

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Background Undescended testis (UDT) is a common urological disorder. Patients with UDT have a risk of malignancy and infertility. The development of these conditions may be due to oxidative stress mediated by reactive oxygen species. The aim of this study was to investigate the relationship between these parameters by detecting oxidative DNA damage (8-hydroxy 2 deoxyguanosine/106 deoxyguanosine), ischemia-modified albumin (IMA), malondialdehyde (MDA), and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) levels in children with UDT and healthy control group. Materials and Methods The blood samples were obtained from 30 patients with UDT and 40 healthy male subjects. The levels of oxidative DNA damage were detected by high-pressure liquid chromatography method. We used commercially available kits that use enzyme-linked immunosorbent assay method to measure IMA, MDA, and NOX4 levels. Results The levels of MDA, IMA, NOX4, and oxidative DNA damage in children with UDT were statistically significantly higher than control group. In addition, we found that the levels of NOX4, IMA, and oxidative DNA damage after 12 months of age was significantly higher than before 12 months of age. Conclusion We identified increased lipid peroxidation, oxidative DNA damage, IMA, and NOX4 levels in children with UDT. Delay in the treatment of UDT may cause oxidative damage. That is why, according to us the antioxidant treatment may be beneficial in children with UDT.

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Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Cited by 54 publications

References 45 publications

Protective Effects of Ipragliflozin, a Sodium-glucose Cotransporter 2 Inhibitor, on a Non-alcoholic Steatohepatitis Mouse Model

Protective Effects of Ipragliflozin, a Sodium-glucose Cotransporter 2 Inhibitor, on a Non-alcoholic Steatohepatitis Mouse Model

Chiroptical spectroscopy and metabolomics for blood‐based sensing of pancreatic cancer

Oxidative DNA Damage and NOX4 Levels in Children with Undescended Testes

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