A high-density linkage map of Theobroma cacao L.

Risterucci, Ange-Marie; Grivet, Laurent; N'Goran, Jeanne A.K.; Pieretti, Isabelle; Flament, Marie-Henriette; Lanaud, Claire

doi:10.1007/s001220051566

Cited by 212 publications

(192 citation statements)

References 40 publications

Supporting

Mentioning

170

Contrasting

Unclassified

Order By: Relevance

“…The primers were pre-developed (Lanaud et al 1999, Risterucci et al 2000 and used to amplify the samples individually and according to a previously described protocol (Santos et al 2007). The amplification products were separated by gel electrophoresis in denaturing polyacrylamide at 5% in an ABI 377 automatic sequencer (Bertolde et al 2009).…”

Section: Methodsmentioning

confidence: 99%

Genetic molecular diversity, production and resistance to witches' broom in cacao clones

Lima¹,

Pereira²,

Pires³

et al. 2013

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic molecular diversity, production and resistance to witches' broom in cacao clones

Lima¹,

Pereira²,

Pires³

et al. 2013

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…For microsatellite analysis, DNA isolation was performed as described in Risterucci et al (2000); after total resuspension, a QIAGEN genomic-tip was used to purify the DNA according to the manufacturer's instructions.…”

Section: Dna Extractionmentioning

confidence: 99%

Cacao domestication I: the origin of the cacao cultivated by the Mayas

et al. 2002

View full text Add to dashboard Cite

Criollo cacao (Theobroma cacao ssp. cacao) was cultivated by the Mayas over 1500 years ago. It has been suggested that Criollo cacao originated in Central America and that it evolved independently from the cacao populations in the Amazon basin. Cacao populations from the Amazon basin are included in the second morphogeographic group: Forastero, and assigned to T. cacao ssp. sphaerocarpum. To gain further insight into the origin and genetic basis of Criollo cacao from Central America, RFLP and microsatellite analyses were performed on a sample that avoided mixing pure Criollo individuals with individuals classified as Criollo but which might have been introgressed with Forastero genes. We distinguished these two types of individuals as Ancient and Modern Criollo. In contrast to previous studies, Ancient Criollo individuals formerly classified as 'wild', were found to

show abstract

“…In the mapping analysis, we did not observe linkage of the three genomic microsatellite loci with the lethal gene 'Luteus-Pa'. These markers map in distinct linkage groups: mTcCIR 15 in group 1, mTcCIR25 in group 6 and mTcCIR 45 in group 8 (Risterucci et al, 2000;Pugh et al, 2004). This demonstrates that 'Luteus-Pa' is not in these genomic regions.…”

Section: Resultsmentioning

confidence: 93%

“…When electrophoresis was carried out on a sequencing gel, with the materials from the genitors, only five of the 35 primers (Lanaud et al, 1999;Risterucci et al, 2000) (mTcCIR 6, mTcCIR 8, mTcCIR 12, mTcCIR 13, and mTcCIR 60) were monomorphic for both genotypes (Pa 30 and Pa 169); 20 were polymorphic. The remaining 10 primers (mTcCIR 1, mTcCIR 7, mTcCIR 11, mTcCIR 17, mTcCIR 22, mTcCIR 28, mTcCIR 33, mTcCIR 42, mTcCIR44, and mTcCIR 57) produced non-informative bands for this cross.…”

Section: Resultsmentioning

confidence: 99%

Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa

Rehem

Almeida²,

Corrêa³

et al. 2010

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The lethal gene 'Luteus-Pa' is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of 'Luteus-Pa' and investigate possible lethal mechanisms. DNA samples of genitors were amplified with 87 SSR and 64 RAPD primers. The SSR primers amplified 65 RAPD primers, giving 179 polymorphic bands. After screening with SSR and RAPD markers, we selected 20 SSR primers, two SSR primers with ESTs and 22 RAPD primers that were polymorphic for genitors Pa 30 and Pa 169. Only two of the 22 RAPD primers and three of the 20 SSR primers were informative and polymorphic in the analysis of the bulk samples of progenies. Among these, primer RAPD E11 produced a band linked to the lethal gene (38.5 cM); none of the SSRs were associated with 'Luteus-Pa'.

show abstract

A high-density linkage map of Theobroma cacao L.

Cited by 212 publications

References 40 publications

Genetic molecular diversity, production and resistance to witches' broom in cacao clones

Genetic molecular diversity, production and resistance to witches' broom in cacao clones

Cacao domestication I: the origin of the cacao cultivated by the Mayas

Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa

Contact Info

Product

Resources

About