A high-affinity Zn2+ uptake system controls growth and biosynthesis of an extracellular, branched β-1,3-β-1,6-glucan inSclerotium rolfsii ATCC 15205

Pilz, Frank; Auling, Georg; Stephan, Do ̈rthe; Rau, Udo; Wagner, Fritz

doi:10.1016/0147-5975(91)90020-e

Cited by 31 publications

(7 citation statements)

References 25 publications

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“…In the first reports, scleroglucan-producing strain conservation was performed by monthly transfers either on PDA or PDY slants (Christias and Lockwood, 1973; Griffith and Compere, 1978; Punja et al, 1982; Pilz et al, 1991). While this is a quite common methodology, an alternatively described technique consists in the preservation of mycelium in sterile distilled water (also known as Castellani’s method; American Type Culture Collection, 1982).…”

Section: Reviewing the Knowledge And Advances On Scleroglucan Productionmentioning

confidence: 99%

Microbial production of scleroglucan and downstream processing

2015

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Synthetic petroleum-based polymers and natural plant polymers have the disadvantage of restricted sources, in addition to the non-biodegradability of the former ones. In contrast, eco-sustainable microbial polysaccharides, of low-cost and standardized production, represent an alternative to address this situation. With a strong global market, they attracted worldwide attention because of their novel and unique physico-chemical properties as well as varied industrial applications, and many of them are promptly becoming economically competitive. Scleroglucan, a β-1,3-β-1,6-glucan secreted by Sclerotium fungi, exhibits high potential for commercialization and may show different branching frequency, side-chain length, and/or molecular weight depending on the producing strain or culture conditions. Water-solubility, viscosifying ability and wide stability over temperature, pH and salinity make scleroglucan useful for different biotechnological (enhanced oil recovery, food additives, drug delivery, cosmetic and pharmaceutical products, biocompatible materials, etc.), and biomedical (immunoceutical, antitumor, etc.) applications. It can be copiously produced at bioreactor scale under standardized conditions, where a high exopolysaccharide concentration normally governs the process optimization. Operative and nutritional conditions, as well as the incidence of scleroglucan downstream processing will be discussed in this chapter. The relevance of using standardized inocula from selected strains and experiences concerning the intricate scleroglucan scaling-up will be also herein outlined.

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Section: Reviewing the Knowledge And Advances On Scleroglucan Productionmentioning

confidence: 99%

Microbial production of scleroglucan and downstream processing

2015

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“…The metal ions are essential coenzymes for enzyme catalytic action, especially enzymes that utilize ATP and synthesize DNA and RNA [30][31][32]. To examine the effects of macro-elements and trace elements on mycelial growth and EPS production, macro-elements and trace elements at a concentration of 1.0 g/L and 0.5 g/L, respectively were added to the media.…”

Section: Effects Of Trace Elementsmentioning

confidence: 99%

Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture

Kwon

Lee

Shin

et al. 2009

Biotechnol Bioproc E

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Both crude exo-biopolymers and mycelial biomass, produced by liquid culture of `çêÇóÅÉéë species, are believed to possess several potential health benefits. As a result of its known biological activities, `çêÇóÅÉéë=ãáäáí~êáë has been extensively characterized in regards to potential medicinal applications. However, optimized liquid culture conditions for enhanced polysaccharide productivity have yet to be developed, which is a necessary step for industrial applications. Therefore, in this study, the liquid culture conditions were optimized for maximal production of mycelial biomass and exo-polysaccharide (EPS) by `K=ãáäáí~êáë. The effects of medium composition, environmental factors, and C/N ratio were investigated. Among these variables 80 g, glucose; 10 g, yeast extract; 0.5 g, MgSO 4 •7H 2 O; and 0.5 g, KH 2 PO 4 in 1 L distilled water were found to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, agitation, and aeration were determined to be 24 o C, uncontrolled pH, 200 rpm, and 1.5 vvm, respectively. Under these optimal conditions, mycelial growth in shake flask cultures and 5 L jar bioreactors was 29.43 and 40.60 g/L, respectively, and polysaccharide production in shake flask cultures and 5 L jar bioreactors was 2.53 and 6.74 g/L, respectively. © KSBB hÉóïçêÇëW=Cordyceps=militaris, ÉñçJéçäóë~ÅÅÜ~êáÇÉI=ãóÅÉäá~ä=ÖêçïíÜI=Å~êÄçåJíçJåáíêçÖÉå=ê~íáç= = = = =

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“…By virtue of its viscous characteristics it is also valuable for other industries (Halleck, 1967). According to the literature the fungus is usually preserved by monthly transfers either on Potato Dextrose Agar (PDA) or on yeast extract supplemented medium (PDY) (Anon., 1982) (Christias & Lockwood, 1973;Griffith & Compere, 1978;Pilz et a/., 1991;Punja et al, 1982). The properties of fungi in culture may be unstable, therefore conditions of preservation and storage should be selected to minimize the risk of such changes (Smith, 1988).…”

Section: Introductionmentioning

confidence: 99%