A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy

Abstract

Rhodamine spirolactams with adjacent amino groups work as acid-resistant and photoswitchable fluorophores in single-molecule localization super-resolution imaging.

Imaging spatiotemporal evolution of molecules and active sites in zeolite catalyst during methanol-to-olefins reaction

“…The super-resolution imaging was carried out using a Nikon N-SIM super-resolution microscopy system with a motorized inverted microscopy ECLIPSE Ti2-E, a ×100/NA 1.49 oil immersion TIRF objective lens (CFI HP) and ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.) 20 , 21 . The wavelengths of illumination and emission detection of SIM used in this work are 405 (detection at 435–485 nm), 488 (detection at 500–545 nm), 561 (detection at 570–640 nm), and 640 nm (detection at 663–738 nm), respectively, which can cover the characteristic area of excitation and emission wavelengths of B n + , N n + , PH n + , and PYR n + .…”

“…Recent work shows that the collective molecular transport could be tracked by use of interference microscopy (IFM) and IRM 5 , 19 in catalysts with crystal size larger than 20 μm 5 , 9 . The confocal fluorescence microscopy (CFM), which shows extraordinary potential in life science 20 , 21 , was demonstrated capable of capturing mobile trajectories of individual conjugated macromolecule in meso- and macro-pore of fluid catalytic cracking (FCC) catalyst pellets 10 , and spatiotemporal evolution of carbonaceous species in model zeolite crystal (larger than 40 μm) in methanol-to-olefins (MTO) and FCC reactions 18 , 22 , 23 . Spatiotemporal-resolved evolution of small molecules (e.g., alkene and alkane) and probe molecules-free measurements of acid sites in catalysts of a few micrometers, which are normally encountered in industrial catalytic processes, remain big challenges 2 , 24 .…”

In vivo real-time tracking of tumor-specific biocatalysis in cascade nanotheranostics enables synergistic cancer treatment

“…[10][11][12] Most of the reported GOD-based biocatalytic strategies have mainly focused on the entrapment of GOD with tumor-specic nanocarriers for prolonged blood circulation duration, increased stability, and improved tumor-targeting ability. [13][14][15][16][17][18][19][20][21] Despite their signicant advances, there remains a lack of desirable GOD-based design strategy to predict in vivo behaviors and regulate the therapeutic efficiency of GOD in clinical applications.…”

Visualizing semipermeability of the cell membrane using a pH-responsive ratiometric AIEgen

“…15 Thanks to the enthusiastic endeavors made by scientists, a lot of uorescent bioprobes have been developed for various applications. [16][17][18][19] Drugs with inherent uorescence make real-time in situ tracking of drug molecules in vivo or in vitro possible, which is of critical importance in pharmaceutical research. However, so far, uorescent drugs as probes for monitoring ion trapping have scarcely been reported in spite of their signicance in studying drug delivery and drug distribution in the body.…”