A green approach for synthesizing silver nanoparticles, and their antibacterial and cytotoxic activities

He, Yangqing; Li, Xing; Zheng, Yuanchuan; Wang, Zhiyan; Ma, Zhen; Yang, Qian; Yao, Binghua; Zhao, Yiming; Zhang, Hao

doi:10.1039/c7nj04224h

Cited by 59 publications

(33 citation statements)

References 39 publications

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“…Furthermore, it has been investigated that nanoparticles appear to attach on to the surface of bacterial cell wall and rupture it, leading to membrane protein denaturation and eventual cell death. Likewise, surface defect in symmetry of NPs is responsible for bacterial inhibition and causes injury to cell (He et al, , ; Ravikumar, Gokulakrishnan, & Boomi, ). Besides, different functional groups attached from RV leaf extract result in capped Cr 2 O 3 NPs which play an important role in bacterial inhibition.…”

Section: Resultsmentioning

confidence: 99%

Facile green synthesis approach for the production of chromium oxide nanoparticles and their different in vitro biological activities

Iqbal

Abbasi

Munir

et al. 2020

Microscopy Res & Technique

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Green synthesis of nanoparticles using plants has become a promising substitute for the conventional chemical synthesis methods. In the present study, our aim was to synthesize chromium oxide nanoparticles (Cr 2 O 3 NPs) through a facile, low-cost, ecofriendly route using leaf extract of Rhamnus virgata (RV). The formation of Cr 2 O 3 NPs was confirmed and characterized by spectroscopic profile of UV-Vis, EDX, FTIR, and XRD analyses. The UV-visible spectroscopy has confirmed the formation of Cr 2 O 3 NPs by the change of color owing to surface plasmon resonance. The bioactive functional groups present in the leaf extract of RV involved in reduction and stabilization of Cr 2 O 3 NPs were determined by FTIR analysis. Based on XRD analysis, crystalline nature of Cr 2 O 3 NPs was determined. The morphological shape and elemental composition of Cr 2 O 3 NPs were investigated using SEM and EDX analyses, respectively. With growing applications of Cr 2 O 3 NPs in biological perspectives, Cr 2 O 3 NPs were evaluated for diverse biopotentials. Cr 2 O 3 NPs were further investigated for its cytotoxicity potentials against HepG2 and HUH-7 cancer cell lines (IC 50 : 39.66 and 45.87 μg/ml), respectively. Cytotoxicity potential of Cr 2 O 3 NPs was confirmed against promastigotes (IC 50 : 33.24 μg/ml) and amastigotes (IC 50 : 44.31 μg/ml) using Leishmania tropica (KMH 23 ). The Cr 2 O 3 NPs were further evaluated for antioxidants, biostatic, alpha-amylase, and protein kinase inhibition properties. Biocompatibility assay was investigated against human macrophages which confirmed the nontoxic nature of Cr 2 O 3 NPs. Overall, the synthesized Cr 2 O 3 NPs are biocompatible and nontoxic and proved to possess significant biopotentials. In future, different in vivo studies are needed to fully investigate the cytotoxicity and mechanism of action associated with these Cr 2 O 3 NPs. K E Y W O R D S anticancer, antileishmanial, antimicrobial, biocompatibility, Cr 2 O 3 NPs, protein kinase

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Section: Resultsmentioning

confidence: 99%

Facile green synthesis approach for the production of chromium oxide nanoparticles and their different in vitro biological activities

Iqbal

Abbasi

Munir

et al. 2020

Microscopy Res & Technique

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“…The biosynthesis of AC-AgNPs was initially determined by observing the change in the reaction mixture from a transparent to a reddish-brown color. The gradual color change in the reaction mixture indicated the formation of AC-AgNPs (Fig 1B) [41, 42]. The previously published articles report that AC contains various beneficial phytochemicals, which have anti-cancer, antioxidative and antibacterial potential [9, 17, 18].…”

Section: Discussionmentioning

confidence: 99%

“…It is expected that the peaks at (3293.12, 1630.26, 1049.58, and 678.17 cm −1 ) in AC extract (Fig 2A) shifted to higher wavenumbers 3295.01, 1634.03, 1059.01, and 683.83 cm −1 respectively in case of AC-AgNPs (Fig 2B). The alterations in the stretching bonds of the AC-AgNPs could be attributed to the reduction, capping, and stabilization process during AgNPs synthesis [41, 42]. In AC-AgNPs the peaks at 3295.01 cm − 1 specifies the existence of strong broad vibrations of O–H stretch, H–bonded bond which belongs to the alcohols and phenols functional group [47, 48].…”

Section: Discussionmentioning

confidence: 99%

Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of silver nanoparticles synthesized using the outer peel extract of Ananas comosus (L.)

et al. 2019

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Currently, green nanotechnology-based approaches using waste materials from food have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the outer peel extract of the fruit Ananas comosus (AC), which is a food waste material. Characterization was done using UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV–visible spectroscopy (at 485 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in AC outer peel extract accountable for the reduction of Ag + ion and the stabilization of AC-AgNPs was investigated through FT-IR. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AC-AgNPs were evaluated for their antioxidative, antidiabetic, and cytotoxic potential against HepG 2 cells along with their antibacterial potential. The results showed that AC-AgNPs are extremely effective with high antidiabetic potential at a very low concentration as well as it exhibited higher cytotoxic activity against the HepG 2 cancer cells in a dose-dependent manner. It also exhibited potential antioxidant activity and moderate antibacterial activity against the four tested foodborne pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AC-AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. Further, it has applications in wound dressing or in treating bacterial related diseases.

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“…43,63,64 Identical values were found in prior studies. 43,65 The XRD data also affirmed the crystalline type of the bio-synthesized Ib1-AgNPs and Ib2-AgNPs. Besides, some unknown peaks were noticed (Figure 4), which suggest that the crystallization of the bioorganic phase could have happened on the peripheral of the biosynthesized Ib1-AgNPs and Ib2-AgNPs, as mentioned in an earlier article.…”

Section: Discussionmentioning

confidence: 59%

<p>Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of <em>Ipomoea batatas</em> (L.) Lam</p>

Das

Patra

Basavegowda

et al. 2019

IJN

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Background: Ipomoea batatas (L.) Lam.(Ib) has high content of various beneficial nutrients which helps in improving and maintaining human health. It is well known as a functional food and also a valuable source of unique natural products. It contains various phenolic and flavonoid bioactive compounds. Methods: In this study, using the outer peel of two varieties of Ib : Korean red skin sweet potato and Korean pumpkin sweet potato, silver nanoparticles (AgNPs) were synthesized (termed Ib1-AgNps and Ib2-AgNps), respectively. Characterization of Ib1-AgNPs and Ib2-AgNPs was carried out through scanning electron microscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive X-ray analysis, X-ray powder diffraction and UV-Vis spectroscopy. Further, the bio-potential of the synthesized AgNPs was investigated by antidiabetic (α-glucosidase assay), antioxidant (free radical scavenging assays), antibacterial (disc diffusion method) and cytotoxicity assays (cell viability against HepG2 cells). Results: FT-IR spectroscopy revealed the contribution of bioactive compounds existing in Ib1 and Ib2 extracts, in the biosynthesis and equilibrium of the AgNPs. Although the Ib2-AgNPs had a higher atomic percentage of Ag in comparison with Ib1-AgNPs, in the antidiabetic assay, the inhibition percentage of α-glucosidase was higher for AgNPs of Ib1 than Ib2, at all three concentrations examined. From the cytotoxicity results, HepG2 cancer cells were more sensitive to the Ib1-AgNPs in comparison to the Ib2-AgNPs-treated HepG2 cells. The antioxidant prospective was higher in Ib2-AgNPs than Ib1-AgNPs. Moreover, the Ib2-AgNPs showed inhibitory action against all five tested pathogenic bacteria, producing an inhibition zone of 8.74-11.52 mm while Ib1-AgNPs had an inhibitory effect on four of them, with an 8.67-11.23 (mm) inhibition zone. Conclusions: Overall, the results concluded that the Ib2-AgNPs exhibited relatively higher functional activity than Ib1-AgNPs, which might be credited to the greater abundance of bioactive compounds existing in Ib2 extract that acted as reducing as well as capping agents in the synthesis of Ib2-AgNPs. Overall, the current study highlights a novel cost-effective and eco-friendly AgNPs synthesis using food waste peels with biocompatibility and could be potentially utilized in biomedical and pharmaceutical industries.

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A green approach for synthesizing silver nanoparticles, and their antibacterial and cytotoxic activities

Abstract: A simple approach using Nelumbo nucifera seeds for synthesizing silver nanoparticles with potential antibacterial and cytotoxic activities was described.

Cited by 59 publications

References 39 publications

Facile green synthesis approach for the production of chromium oxide nanoparticles and their different in vitro biological activities

Facile green synthesis approach for the production of chromium oxide nanoparticles and their different in vitro biological activities

Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of silver nanoparticles synthesized using the outer peel extract of Ananas comosus (L.)

<p>Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of <em>Ipomoea batatas</em> (L.) Lam</p>

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