A glucose oxidase-coupled DNAzyme sensor for glucose detection in tears and saliva

Liu, Chengcheng; Sheng, Yongjie; Sun, Yanhong; Feng, Junkui; Wang, Shijin; Zhang, Jin; Xu, Jiafang; Jiang, Dong

doi:10.1016/j.bios.2015.03.070

Cited by 95 publications

(45 citation statements)

References 42 publications

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“…Glucose biosensor is the most common enzymatic biosensor. The enzyme used for the detection of glucose is glucose oxidase (GOx)2324252627. GOx catalyses the oxidation of glucose into gluconolactone and hydrogen peroxide28.…”

mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

304

187

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A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM−1 cm−2 and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

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mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

304

187

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“…Such a design was applied for the human lgG detection, with a detection limit of 2 fg/mL (3×10 -17 M). In another study, DNAzyme was coupled with glucose oxidase (GOx) for sub-mM glucose detection in biological samples 126. This sensor was designed based on the fact that the DNAzyme required both Cu 2+ and H 2 O 2 for activity 127, 128.…”

Section: Sensing Other Analytesmentioning

confidence: 99%

Theranostic DNAzymes

Zhou¹,

Ding²,

Liu³

2017

Theranostics

204

145

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DNAzymes are catalytically active DNA molecules that are obtained via in vitro selection. RNA-cleaving DNAzymes have attracted significant attention for both therapeutic and diagnostic applications due to their excellent programmability, stability, and activity. They can be designed to cleave a specific mRNA to down-regulate gene expression. At the same time, DNAzymes can sense a broad range of analytes. By combining these two functions, theranostic DNAzymes are obtained. This review summarizes the progress of DNAzyme for theranostic applications. First, in vitro selection of DNAzymes is briefly introduced, and some representative DNAzymes related to biological applications are summarized. Then, the applications of DNAzyme for RNA cleaving are reviewed. DNAzymes have been used to cleave RNA for treating various diseases, such as viral infection, cancer, inflammation and atherosclerosis. Several formulations have entered clinical trials. Next, the use of DNAzymes for detecting metal ions, small molecules and nucleic acids related to disease diagnosis is summarized. Finally, the theranostic applications of DNAzyme are reviewed. The challenges to be addressed include poor DNAzyme activity under biological conditions, mRNA accessibility, delivery, and quantification of gene expression. Possible solutions to overcome these challenges are discussed, and future directions of the field are speculated.

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“…The painful finger-stick is the main drawback leading to patient non-compliance and also makes it impossible to achieve real-time information required for some biomedical applications. Therefore, major scientific effort is being made to develop non-invasive glucose monitoring diagnostic tools (Liu et al, 2015; Lodgotra et al, 2016; Rabti et al, 2016). For instance, human perspiration offers valuable information about health status depending on the levels of several analytes, like lactate, ammonium, and glucose (Labroo and Cui, 2013; Mitsubayashi et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

A stretchable and screen-printed electrochemical sensor for glucose determination in human perspiration

Abellán-Llobregat

Jeerapan

Bandodkar

et al. 2017

Biosensors and Bioelectronics

287

155

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Here we present two types of all-printable, highly stretchable, and inexpensive devices based on platinum (Pt)-decorated graphite for glucose determination in physiological fluids. Said devices are: a non-enzymatic sensor and an enzymatic biosensor, the latter showing promising results. Glucose has been quantified by measuring hydrogen peroxide (H2O2) reduction by chronoamperometry at -0.35 V (vs pseudo-Ag/AgCl) using glucose-oxidase immobilized on Pt-decorated graphite. The sensor performs well for the quantification of glucose in phosphate buffer solution (0.25 M PBS, pH 7.0), with a working range between 33 μM and 0.9 mM, high sensitivity and selectivity, and a low limit of detection (LOD). Thus it provides an alternative non-invasive and on-body quantification of glucose levels in human perspiration. This biosensor has been successfully applied on real human perspiration samples and results also show a significant correlation between glucose concentration in perspiration and glucose concentration in blood measured by a commercial glucose meter.

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A glucose oxidase-coupled DNAzyme sensor for glucose detection in tears and saliva

Cited by 95 publications

References 42 publications

Novel amperometric glucose biosensor based on MXene nanocomposite

Novel amperometric glucose biosensor based on MXene nanocomposite

Theranostic DNAzymes

A stretchable and screen-printed electrochemical sensor for glucose determination in human perspiration

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