A Genetic System Involving Superoxide Causes F1 Necrosis in Wheat (T. aestivumL.)

Khanna‐Chopra, Renu; Dalal, Monika; Kumar, Pratap; Laloraya, Malini

doi:10.1006/bbrc.1998.9043

Cited by 23 publications

(11 citation statements)

References 14 publications

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“…ROS production was previously reported to occur in wheat hybrid necrosis (Khanna-Chopra et al, 1998). The DCFstaining patterns were compared in the epidermal cell layers of the 3rd leaves from the top of 2-week-old seedlings, showing that the number of H 2 O 2 -produced cells in both necrosis lines was much larger than that in wildtype plants (Fig.…”

Section: Ros Production In the Necrosis Linesmentioning

confidence: 73%

“…The transcript level of another mitochondrial ribosomal protein gene, Whlp (Mizumoto et al, 2004), was gradually reduced according to the necrotic symptom levels, although no significant changes were found for the other mitochondrial ribosomal protein gene, TaMRPL11 (Handa et al, 2001). Khanna-Chopra et al (1998) previously reported that the gradient of superoxide generation in leaves of the wheat hybrid necrosis was parallel to the progression of necrosis symptom. The expression levels of the wheat antioxidant enzyme genes which encode mitochondrial manganese superoxide dismutase (MnSOD) and chloroplastic Cu/Zn superoxide dismutase (Cu/ZnSOD) do not show any change among examined leaves at the heading stage (Baek and Skinner, 2003).…”

Section: Transcript Accumulation Levels In the Necrosis Linesmentioning

confidence: 91%

“…These two dominant complementary genes Ne1 and Ne2 are located on chromosome arms 5BL and 2BS, respectively (Tsunewaki, 1960;1992;Nishikawa et al, 1974;Chu et al, 2006). Leaves of the necrotic hybrid wheat exhibit high superoxide content (Khanna-Chopra et al, 1998;Dalal and Khanna-Chopra, 1999), and show increased activities of some antioxidant enzymes (Dalal and Khanna-Chopra, 2001). We previously isolated two non-homoeologous genes, Waox1a and Waox1c, encoding the AOX proteins from common wheat (Takumi et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

2007

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Mitochondrial alternative oxidase (AOX) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in plants. AOX is a key enzyme of the alternative respiration pathway. To study the effects of necrotic cell death on the mitochondrial function, production of reactive oxygen species (ROS), respiration capacities and accumulation patterns of mitochondriatargeted protein-encoding gene transcripts were compared between wild-type, lesion-mimic mutant and hybrid necrosis wheat plants. Around cells with the necrosis symptom, ROS accumulated abundantly in the intercellular spaces. The ratio of the alternative pathway to the cytochrome pathway was markedly enhanced in the necrotic leaves. Transcripts of a wheat AOX gene, Waox1a, were more abundant in a novel lesion-mimic mutant of common wheat than in the wildtype plants. An increased level of the Waox1a transcripts was also observed in hybrid plants containing Ne1 and Ne2 genes. These results indicated that an increase of the wheat AOX transcript level resulted in enhancement of respiration capacity of the alternative pathway in the necrotic cells.

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Section: Ros Production In the Necrosis Linesmentioning

confidence: 73%

Section: Transcript Accumulation Levels In the Necrosis Linesmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

2007

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“…It has been suggested that hybrid necrosis is due to genetic incompatibilities that involve an immune response (Bomblies and Weigel, 2007). Wheat plants undergoing hybrid necrosis were found to have increased levels of superoxide (a molecule involved in oxidative burst and hypersensitive response) (Khanna-Chopra et al , 1998). Elevated levels of PR proteins and defence response genes have also been associated with hybrid necrosis (Bomblies et al , 2007).…”

Section: Discussionmentioning

confidence: 99%

Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls

Cookson¹,

Clemente‐Moreno²,

Hévin³

et al. 2014

Journal of Experimental Botany

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“…The involvement of the plant immune system in several independent cases of hybrid necrosis implicates aberrant activation of disease resistance signaling as a general mechanism. The phenotypic similarities of hybrid necrosis cases in other species to those we describe in A. thaliana, including in the expression of defense-related genes [8,9], suggest that A. thaliana is an accessible model for studying the molecular causes of this type of genetic incompatibility [2].…”

Section: Introductionmentioning

confidence: 99%

Autoimmune Response as a Mechanism for a Dobzhansky-Muller-Type Incompatibility Syndrome in Plants

et al. 2007

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Epistatic interactions between genes are a major factor in evolution. Hybrid necrosis is an example of a deleterious phenotype caused by epistatic interactions that is observed in many intra- and interspecific plant hybrids. A large number of hybrid necrosis cases share phenotypic similarities, suggesting a common underlying mechanism across a wide range of plant species. Here, we report that approximately 2% of intraspecific crosses in Arabidopsis thaliana yield F1 progeny that express necrosis when grown under conditions typical of their natural habitats. We show that several independent cases result from epistatic interactions that trigger autoimmune-like responses. In at least one case, an allele of an NB-LRR disease resistance gene homolog is both necessary and sufficient for the induction of hybrid necrosis, when combined with a specific allele at a second locus. The A. thaliana cases provide insights into the molecular causes of hybrid necrosis, and serve as a model for further investigation of intra- and interspecific incompatibilities caused by a simple epistatic interaction. Moreover, our finding that plant immune-system genes are involved in hybrid necrosis suggests that selective pressures related to host–pathogen conflict might cause the evolution of gene flow barriers in plants.

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A Genetic System Involving Superoxide Causes F1 Necrosis in Wheat (T. aestivumL.)

Cited by 23 publications

References 14 publications

Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls

Autoimmune Response as a Mechanism for a Dobzhansky-Muller-Type Incompatibility Syndrome in Plants

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